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Immunohistological Detection of Active Satellite Cellsin Rat Dorsal Root Ganglia after Parenteral Administration of Lipopolysaccharide and during Aging

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Bulletin of Experimental Biology and Medicine Aims and scope

Immunohistochemical reaction to glial fibrillar acid protein (GFAP) is widely used for identification of activated satellite cells in sensory ganglia. We used this marker in studies of satellite cells activation in dorsal root ganglia during aging and under conditions of experimental systemic inflammation: in young (4 months) and aged (18-19 months) rats and animals with experimental LPS-induced systemic inflammation. The number of GFAP+ satellite cells increased significantly after parenteral injection of LPS and during aging, which can indicate similarity of mechanisms of reactive glial changes during aging and systemic inflammation.

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References

  1. Kolos EA, Korzhevskii DE. Activation of Microglyocytes in the Anterior Horns of Rat Spinal Cord After Administration of Bacterial Lipopolysaccharide. Bull. Exp. Biol. Med. 2017;163(4):515-518. doi: https://doi.org/10.1007/s10517-017-3841-8

    Article  CAS  PubMed  Google Scholar 

  2. Belzer V, Hanani M. Nitric oxide as a messenger between neurons and satellite glial cells in dorsal root ganglia. Glia. 2019;67(7):1296-1307.

    PubMed  Google Scholar 

  3. Blum E, Procacci P, Conte V, Hanani M. Systemic inflammation alters satellite glial cell function and structure. A possible contribution to pain. Neuroscience. 2014;274:209-217.

    CAS  PubMed  Google Scholar 

  4. Blum E, Procacci P, Conte V, Sartori P, Hanani M. Long term effects of lipopolysaccharide on satellite glial cells in mouse dorsal root ganglia. Exp. Cell Res. 2017;350(1):236-241.

    CAS  PubMed  Google Scholar 

  5. Chung HY, Kim DH, Lee EK, Chung KW, Chung S, Lee B, Seo AY, Chung JH, Jung YS, Im E, Lee J, Kim ND, Choi YJ, Im DS, Yu BP. Redefining chronic inflammation in aging and age-related diseases: proposal of the senoinflammation concept. Aging Dis. 2019;10(2):367-382.

    PubMed  PubMed Central  Google Scholar 

  6. Feldman-Goriachnik R, Belzer V, Hanani M. Systemic inflammation activates satellite glial cells in the mouse nodose ganglion and alters their functions. Glia. 2015;63(11):2121-2132.

    PubMed  Google Scholar 

  7. Franceschi C, Capri M, Monti D, Giunta S, Olivieri F, Sevini F, Panourgia MP, Invidia L, Celani L, Scurti M, Cevenini E, Castellani GC, Salvioli S. Inflammaging and anti-inflammaging: a systemic perspective on aging and longevity emerged from studies in humans. Mech. Ageing Dev. 2007;128(1):92-105.

    CAS  PubMed  Google Scholar 

  8. Hanani M. Satellite glial cells in sensory ganglia: from form to function. Brain Res. Brain Res. Rev. 2005;48(3):457-476.

    CAS  PubMed  Google Scholar 

  9. Hanani M, Blum E, Liu S, Peng L, Liang S. Satellite glial cells in dorsal root ganglia are activated in streptozotocin-treated rodents. J. Cell. Mol. Med. 2014;18(12):2367-2371.

    CAS  PubMed  PubMed Central  Google Scholar 

  10. Hung Y, Suzuki K. The pattern recognition receptors and lipopolysaccharides (LPS)-induced systemic inflammation. Int. J. Res. Studies Med. Health Sci. 2017;2(7):1-7.

    Google Scholar 

  11. Michaud M, Balardy L, Moulis G, Gaudin C, Peyrot C, Vellas B, Cesari M, Nourhashemi F. Proinflammatory cytokines, aging, and age-related diseases. J. Am. Med. Dir. Assoc. 2013;14(12):877-882.

    PubMed  Google Scholar 

  12. Miller KE, Richards BA, Kriebel RM. Glutamine-, glutamine synthetase-, glutamate dehydrogenase- and pyruvate carboxylase-immunoreactivities in the rat dorsal root ganglion and peripheral nerve. Brain Res. 2002;945(2):202-211.

    CAS  PubMed  Google Scholar 

  13. Pannese E. Biology and pathology of perineuronal satellite cells in sensory ganglia. Springer International Publishing, 2018.

  14. Seemann S, Zohles F, Lupp A. Comprehensive comparison of three different animal models for systemic inflammation. J. Biomed. Sci. 2017;24(1):60. doi: https://doi.org/10.1186/s12929-017-0370-8

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Xia S, Zhang X, Zheng S, Khanabdali R, Kalionis B, Wu J, Wan W, Tai X. An update on inflamm-aging: mechanisms, prevention, and treatment. J. Immunol. Res. 2016;2016. ID 8426874. doi: https://doi.org/10.1155/2016/8426874

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Authors and Affiliations

  1. Institute of Experimental Medicine, St. Petersburg, Russia

    E. A. Kolos & D. E. Korzhevskii

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  1. E. A. Kolos
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  2. D. E. Korzhevskii
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Correspondence to E. A. Kolos.

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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 169, No. 5, pp. 596-599, May, 2020

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Kolos, E.A., Korzhevskii, D.E. Immunohistological Detection of Active Satellite Cellsin Rat Dorsal Root Ganglia after Parenteral Administration of Lipopolysaccharide and during Aging. Bull Exp Biol Med 169, 665–668 (2020). https://doi.org/10.1007/s10517-020-04950-2

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  • DOI: https://doi.org/10.1007/s10517-020-04950-2

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