Abstract
An ultrasound-assisted deep eutectic solvent-based liquid-phase microextraction (UA-DES-LPME) was established, for the first time, for the extraction of carbamazepine (CBZ) from human plasma samples. The extract was determined by HPLC-ultraviolet detection (HPLC–UV). A deep eutectic solvent (DES) was readily synthesized by mixing choline chloride and phenol (ChCl:Ph) in the mole ratio of 1:2 in ambient temperature and used without any further purification. In the present method, THF was used as an emulsifier agent. Some parameters, including the DES components mole ratio, kind of emulsifier agent, and salt addition effect, were optimized by a one-at-a-time approach. Central composite design (CCD) was used to optimize the rest of the effective parameters, including DES volume, pH, ultrasonication time, and THF volume. The optimum conditions were found to be: 11.78 pH, 314 µL DES volume, 523 µL THF volume, and 9.0 min ultrasonication time. The performance of the method was evaluated under optimum conditions, and the method exhibited good linearity over a concentration range of 10–1500 ng mL−1, low limit of detection and quantitation, good precision, and high extraction recovery. Finally, the applicability of the method was assessed by analysis of the spiked plasma samples, and the results have shown high relative recoveries with good precision.
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References
https://www.drugbank.ca/drugs/DB00564. April 11, 2020
Chen C, Wang Y, Ding S, Hong C, Wang Z (2019) A novel sensitive and selective electrochemical sensor based on integration of molecularly imprinted with hollow silver nanospheres for determination of carbamazepine. Microchem J 147:191–197. https://doi.org/10.1016/j.microc.2019.03.024
Brunton L, Chabner B, Knollmann B (2018) Goodman & Gilman’s The Pharmacological Basis of THERAPEUTICS. In: Brunton L (ed) Goodman and Gilman’s The Pharmacological Basis of THERAPEUTICS. McGraw-Hill, Newyork
Rezaee M, Assadi Y, Milani Hosseini M-R, Aghaee E, Ahmadi F, Berijani S (2006) Determination of organic compounds in water using dispersive liquid–liquid microextraction. J Chromatogr A 1116(1):1–9. https://doi.org/10.1016/j.chroma.2006.03.007
Behbahani M, Najafi F, Bagheri S, Bojdi MK, Salarian M, Bagheri A (2013) Application of surfactant assisted dispersive liquid–liquid microextraction as an efficient sample treatment technique for preconcentration and trace detection of zonisamide and carbamazepine in urine and plasma samples. J Chromatogr A 1308:25–31. https://doi.org/10.1016/j.chroma.2013.07.088
Parvin S, Aghamohammadi M, Fallahi E, Kalhor H (2020) Simultaneous determination of lamotrigine and carbamazepine in plasma using ultrasound-assisted emulsification Microextraction-high performance liquid chromatography. J Anal Chem 75(5):622–628. https://doi.org/10.1134/S1061934820050160
Rezaee M, Mashayekhi HA (2012) Solid-phase extraction combined with dispersive liquid-liquid microextraction as an efficient and simple method for the determination of carbamazepine in biological samples. Anal Methods-UK 4(9):2887–2892. https://doi.org/10.1039/c2ay25460c
Abbott AP, Boothby D, Capper G, Davies DL, Rasheed RK (2004) Deep eutectic solvents formed between choline chloride and carboxylic acids: versatile alternatives to ionic liquids. J Am Chem Soc 126(29):9142–9147. https://doi.org/10.1021/ja048266j
Pena-Pereira F, Namieśnik J (2014) Ionic liquids and deep eutectic mixtures: sustainable solvents for extraction processes. Chemsuschem 7(7):1784–1800. https://doi.org/10.1002/cssc.201301192
Shishov A, Bulatov A, Locatelli M, Carradori S, Andruch V (2017) Application of deep eutectic solvents in analytical chemistry. A Rev Microchem J 135:33–38. https://doi.org/10.1016/j.microc.2017.07.015
Heidari H, Ghanbari-Rad S, Habibi E (2020) Optimization deep eutectic solvent-based ultrasound-assisted liquid-liquid microextraction by using the desirability function approach for extraction and preconcentration of organophosphorus pesticides from fruit juice samples. J Food Compos Anal 87:103389. https://doi.org/10.1016/j.jfca.2019.103389
Shishov AY, Chislov MV, Nechaeva DV, Moskvin LN, Bulatov AV (2018) A new approach for microextraction of non-steroidal anti-inflammatory drugs from human urine samples based on in-situ deep eutectic mixture formation. J Mol Liq 272:738–745. https://doi.org/10.1016/j.molliq.2018.10.006
Shishov A, Volodina N, Nechaeva D, Gagarinova S, Bulatov A (2019) An automated homogeneous liquid-liquid microextraction based on deep eutectic solvent for the HPLC-UV determination of caffeine in beverages. Microchem J 144:469–473. https://doi.org/10.1016/j.microc.2018.10.014
Deng W, Yu L, Li X, Chen J, Wang X, Deng Z, Xiao Y (2019) Hexafluoroisopropanol-based hydrophobic deep eutectic solvents for dispersive liquid-liquid microextraction of pyrethroids in tea beverages and fruit juices. Food Chem 274:891–899. https://doi.org/10.1016/j.foodchem.2018.09.048
Safavi A, Ahmadi R, Ramezani AM (2018) Vortex-assisted liquid-liquid microextraction based on hydrophobic deep eutectic solvent for determination of malondialdehyde and formaldehyde by HPLC-UV approach. Microchem J 143:166–174. https://doi.org/10.1016/j.microc.2018.07.036
Jouyban A, Farajzadeh MA, Afshar Mogaddam MR (2018) A lighter-than-water deep eutectic-solvent-based dispersive liquid-phase microextraction method in a U-shaped homemade device. New J Chem 42(12):10100–10110. https://doi.org/10.1039/C8NJ00597D
Asiabi H, Yamini Y, Shamsayei M, Mehraban JA (2018) A nanocomposite prepared from a polypyrrole deep eutectic solvent and coated onto the inner surface of a steel capillary for electrochemically controlled microextraction of acidic drugs such as losartan. Microchim Act 185(3):169. https://doi.org/10.1007/s00604-018-2684-y
Kanberoglu GS, Yilmaz E, Soylak M (2018) Usage of deep eutectic solvents for the digestion and ultrasound-assisted liquid phase microextraction of copper in liver samples. J Iran Chem Soc 15(10):2307–2314. https://doi.org/10.1007/s13738-018-1419-7
Kumps A (1984) Simultaneous HPLC determination of Oxcarbazepine, carbamazepine and their metabolites in serum. J Liq Chromatogr 7(6):1235–1241. https://doi.org/10.1080/01483918408074040
Khezeli T, Daneshfar A, Sahraei R (2015) Emulsification liquid–liquid microextraction based on deep eutectic solvent: An extraction method for the determination of benzene, toluene, ethylbenzene and seven polycyclic aromatic hydrocarbons from water samples. J Chromatogr A 1425:25–33. https://doi.org/10.1016/j.chroma.2015.11.007
Psillakis E, Kalogerakis N (2002) Developments in single-drop microextraction. TrAC Trends Anal Chem 21(1):54–64. https://doi.org/10.1016/S0165-9936(01)00126-1
Hashemi M, Jahanshahi N, Habibi A (2012) Application of ultrasound-assisted emulsification microextraction for determination of benzene, toluene, ethylbenzene and o-xylene in water samples by gas chromatography. Desalination 288:93–97. https://doi.org/10.1016/j.desal.2011.12.017
Jones OAH, Voulvoulis N, Lester JN (2002) Aquatic environmental assessment of the top 25 English prescription pharmaceuticals. Water Res 36(20):5013–5022. https://doi.org/10.1016/S0043-1354(02)00227-0
Heidari H, Limouei-Khosrowshahi B (2019) Magnetic solid phase extraction with carbon-coated Fe3O4 nanoparticles coupled to HPLC-UV for the simultaneous determination of losartan, carvedilol, and amlodipine besylate in plasma samples. J Chromatogr B 1114–1115:24–30. https://doi.org/10.1016/j.jchromb.2019.03.025
Heidari H, Razmi H, Jouyban A (2014) Desirability function approach for the optimization of an in-syringe ultrasound-assisted emulsification-microextraction method for the simultaneous determination of amlodipine and nifedipine in plasma samples. J Sep Sci 37(12):1467–1474. https://doi.org/10.1002/jssc.201400030
ICH Harmonised Tripartite Guideline. Validation of Analytical Procedures: Text and Methodology Q2 (R1), International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use, 1994
Franceschi L, Furlanut M (2005) A simple method to monitor plasma concentrations of oxcarbazepine, carbamazepine, their main metabolites and lamotrigine in epileptic patients. Pharmacol Res 51(4):297–302. https://doi.org/10.1016/j.phrs.2004.09.008
Wang R, Cui Y, Hu F, Liu W, Du Q, Zhang Y, Zha J, Huang T, Fizir M, He H (2019) Selective recognition and enrichment of carbamazepine in biological samples by magnetic imprinted polymer based on reversible addition-fragmentation chain transfer polymerization. J Chromatogr A 1591:62–70. https://doi.org/10.1016/j.chroma.2019.01.057
Shokry E, Villanelli F, Malvagia S, Rosati A, Forni G, Funghini S, Ombrone D, Della Bona M, Guerrini R, la Marca G (2015) Therapeutic drug monitoring of carbamazepine and its metabolite in children from dried blood spots using liquid chromatography and tandem mass spectrometry. J Pharm Biomed Anal 109:164–170. https://doi.org/10.1016/j.jpba.2015.02.045
Wu S, Xu W, Subhani Q, Yang B, Chen D, Zhu Y, Li L (2012) Ion chromatography combined with online electrochemical derivatization and fluorescence detection for the determination of carbamazepine in human plasma. Talanta 101:541–545. https://doi.org/10.1016/j.talanta.2012.09.039
Zhang J, Liu D, Meng X, Shi Y, Wang R, Xiao D, He H (2017) Solid phase extraction based on porous magnetic graphene oxide/β-cyclodextrine composite coupled with high performance liquid chromatography for determination of antiepileptic drugs in plasma samples. J Chromatogr A 1524:49–56. https://doi.org/10.1016/j.chroma.2017.09.074
Fortuna A, Sousa J, Alves G, Falcão A, Soares-da-Silva P (2010) Development and validation of an HPLC-UV method for the simultaneous quantification of carbamazepine, oxcarbazepine, eslicarbazepine acetate and their main metabolites in human plasma. Anal Bioanal Chem 397(4):1605–1615. https://doi.org/10.1007/s00216-010-3673-0
Queiroz RHC, Bertucci C, Malfará WR, Dreossi SAC, Chaves AR, Valério DAR, Queiroz MEC (2008) Quantification of carbamazepine, carbamazepine-10,11-epoxide, phenytoin and phenobarbital in plasma samples by stir bar-sorptive extraction and liquid chromatography. J Pharm Biomed Anal 48(2):428–434. https://doi.org/10.1016/j.jpba.2008.03.020
Kim K-B, Seo K-A, Kim S-E, Bae SK, Kim D-H, Shin J-G (2011) Simple and accurate quantitative analysis of ten antiepileptic drugs in human plasma by liquid chromatography/tandem mass spectrometry. J Pharm Biomed Anal 56(4):771–777. https://doi.org/10.1016/j.jpba.2011.07.019
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Heidari, H., Yari, B. Multivariate Optimization of an Ultrasound-Assisted Deep Eutectic Solvent-Based Liquid-Phase Microextraction Method for HPLC–UV Analysis of Carbamazepine in Plasma. Chromatographia 83, 1467–1475 (2020). https://doi.org/10.1007/s10337-020-03966-0
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DOI: https://doi.org/10.1007/s10337-020-03966-0