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Suppression of long non-coding RNA PCAT19 inhibits glioma cell proliferation and invasion, and increases cell apoptosis through regulation of MELK targeted by miR-142-5p

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Abstract

Background

Glioma has the chief type of primary brain tumors worldwide. The glioma may be controlled by regulators including some lncRNAs, miRNAs, and proteins.

Objective

Our study aims to discover the underlying mechanism for lncPCAT19/miR-142-5p/MELK axis in glioma progression.

Methods

The clinical samples were from patients with gliomas in our Hospital. Hematoxylin–eosin staining (H&E) was applied to determine the clinical pathological changes. Real time PCR was performed to measure the levels of lncPCAT19, miR-142-5p, MELK, and expression of other genes. Western blot was conducted to detect the protein level of MELK. RIP assay was performed to analyze the interaction between lncPCAT19 and miR-142-5p, and dual-luciferase reporter assay was used to determine the binding site between lncPCAT19 and miR-142-5p. CCK-8, colony formation assay, flow cytometry, and trans-well assay were carried out to confirm cell proliferation, colony formation, apoptosis, and invasion, respectively.

Results

LncPCAT19 was increased in cancer tissues. Then, lncPCAT19 could interact with and down-regulate miR-142-5p. Knockdown of lncPCAT19 distinctly inhibited tumor growth in vivo. Interfering lncPCAT19/overexpression of miR-142-5p decreased glioma cell proliferation, colony formation and invasion, and promoted cell apoptosis by down-regulating expression of Cyclin B1, CDK2, N-cadherin, Bcl-2, and by up-regulating expression of Bax and E-cadherin. Moreover, overexpression of lncPCAT19 overturned tumor-suppressing role of miR-142-5p in cells. Additionally, lncPCAT19 and miR-142-5p synergistically regulated expression of MELK. In conclusion, lncPCAT19 enhanced glioma development via increasing MELK by performing as a sponge of miR-142-5p.

Conclusions

LncPCAT19 promotes glioma progression by sponging miR-142-5p to upregulate MELK levels. Thus, lncPCAT19/miR-142-5p/MELK signaling would be a potential target for glioma treatment.

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Acknowledgements

We thank our team for technical cooperation.

Funding

This study received no financial supporting from government subjects.

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Authors and Affiliations

  1. Department of Rehabilitation Medicine, Chenzhou No. 1 People’s Hospital, Luo Jia Jin Street, Chenzhou, 423000, Hunan, China

    Yu-hua Xie

  2. Emergency Department, Chenzhou No. 1 People’s Hospital, Luo Jia Jin Street, Chenzhou, 423000, Hunan, China

    Jiao Hu

Authors
  1. Yu-hua Xie
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  2. Jiao Hu
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Contributions

Jiao Hu designed the project. Yu-hua Xie and Jiao Hu performed the animal experiments, and Yu-hua Xie also cultured the cell lines and conducted the cell experiments. Yu-hua Xie analyzed the experimental data. Jiao Hu wrote the manuscript, and he offered discussion and suggestions. Jiao Hu provided the financial support.

Corresponding author

Correspondence to Jiao Hu.

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Conflict of interest

Yu-hua Xie and Jiao Hu declare that they have no conflict of interest.

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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All procedures performed in our studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Informed consent was obtained from all included participants.

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Xie, Yh., Hu, J. Suppression of long non-coding RNA PCAT19 inhibits glioma cell proliferation and invasion, and increases cell apoptosis through regulation of MELK targeted by miR-142-5p. Genes Genom 42, 1299–1310 (2020). https://doi.org/10.1007/s13258-020-01003-w

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  • DOI: https://doi.org/10.1007/s13258-020-01003-w

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