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Leaf anatomy and histochemistry of secretory structures of Zanthoxylum caribaeum Lam. (Rutaceae)

  • Structural Botany - Original Article
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Abstract

Rutaceae species, especially of the genus Zanthoxylum L., are known for possessing a well-diversified chemical composition with high potential for pharmacological activity. A variety of Zanthoxylum species are distributed throughout Brazil. This study aimed to characterize the leaf anatomy and identify the histochemical composition of the secretory material from the secretory structures of Z. caribaeum leaves. Leaves of Z. caribaeum were collected and processed for anatomical, histochemical and micromorphological characterization following standard methodologies. The leaves have an unstratified epidermis and glandular trichomes in epidermal depressions on the abaxial surface. Extrafloral nectaries were also observed on the abaxial surface. The leaf is hypostomatic and the stomata are anomocytic. The mesophyll is dorsiventral and has secretory cavities. The mesophyll cavities produce a secretion of lipidic nature and the cavities on the leaf margin produce a secretion of mixed nature, with neutral lipids and essences. In summary, our findings show that the leaves of Z. caribaeum have secretory structures like glandular trichomes that can function as extrafloral nectaries and cavities. The characterization of the leaf anatomy of Z. caribaeum can provide relevant data for future taxonomic studies of the genus.

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Acknowledgements

The authors thank CAPES for the scholarship, Dr. Milton Groppo Junior for identifying the species, and the staff at the Laboratório de Anatomia e Histoquímica Vegetal and the Núcleo de Microscopia e Microanálise of Universidade Federal do Recôncavo da Bahia for the support during this study.

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Authors and Affiliations

  1. Universidade Estadual de Feira de Santana, Feira de Santana, Bahia, 44036-900, Brazil

    Carine Raisa Barbosa de Andrade, Hugo Neves Brandão & Clayton Queiroz Alves

  2. Universidade Federal do Recôncavo da Bahia, Cruz das Almas, Bahia, 44380-000, Brazil

    Fabiano Machado Martins & Larisse de Freitas-Silva

Authors
  1. Carine Raisa Barbosa de Andrade
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  2. Fabiano Machado Martins
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  3. Hugo Neves Brandão
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  4. Clayton Queiroz Alves
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  5. Larisse de Freitas-Silva
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Contributions

FM, HN and CQ conceived and designed the study. CR conducted experiments and biochemical and metabolite analyses. CR, FM, HN, CQ, LF discussed the results and participated in preparing the manuscript. CR and LF wrote the manuscript. All authors read, improved and approved the manuscript.

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Correspondence to Larisse de Freitas-Silva.

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de Andrade, C.R.B., Martins, F.M., Brandão, H.N. et al. Leaf anatomy and histochemistry of secretory structures of Zanthoxylum caribaeum Lam. (Rutaceae). Braz. J. Bot 43, 961–968 (2020). https://doi.org/10.1007/s40415-020-00658-9

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  • DOI: https://doi.org/10.1007/s40415-020-00658-9

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