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Gene Analysis of Genetically Modified Soybean Lectin Based on Fluorescence Quantitative PCR

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Abstract

Considering that the genetically modified soybean lectin gene is affected by the gene type, to improve the stability of the genetically modified soybean lectin gene, a method based on fluorescence quantitative PCR to analyze the genetic characteristics of the genetically modified soybean lectin was proposed. The common soybean varieties, Wangshuibai and Huangdou No. 3 were selected as materials for tissue-specific expression analysis. Under the background conditions of analyzing the genetically modified soybean lectin genes, fluorescent quantitative PCR was applied to the analysis of genetic characteristics. The characteristics of the genetically modified soybean lectin gene were analyzed in terms of location characteristics and expression characteristics. The results showed that the soybean lectin gene has a complex functional mechanism and may participate in a variety of stress-related regulatory or signal transduction pathways in different ways; Lectin2.1 transcripts are expressed in abundance in glume and lemma in seedling tips, Lectin2.2 was mainly expressed in the roots, and a small amount was expressed in leaves and lemma; Lectin2.1 and Lectin2.2 are highly similar in nucleic acid and amino acid composition, and have similar subcellular localization characteristics.

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Funding

Key Projects of Jilin Province Science and Technology Development Plan (No. 20180201051NY).

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Authors and Affiliations

  1. College of Food Science and Engineering, Jilin Agricultural University, Changchun, 130118, China

    Zhaohui Wang, Hongyu Jia & Hansong Yu

  2. Department of Basic Courses, NCO Institute of Army Academy of Armored Forces, Changchun, 130117, China

    Tong Wang

  3. School of Life Sciences, Changchun University of Science and Technology, Changchun, 130600, China

    Bin Yang

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  1. Zhaohui Wang
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  2. Hongyu Jia
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  3. Hansong Yu
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  4. Tong Wang
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  5. Bin Yang
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Contributions

Software, ZW and HJ; supervision, BY; writing, review and editing, TW and HY; Research, ZW and HJ; All authors have read and agreed to the published version of the manuscript.

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Correspondence to Bin Yang.

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Wang, Z., Jia, H., Yu, H. et al. Gene Analysis of Genetically Modified Soybean Lectin Based on Fluorescence Quantitative PCR. Biochem Genet 59, 185–201 (2021). https://doi.org/10.1007/s10528-020-09998-3

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  • DOI: https://doi.org/10.1007/s10528-020-09998-3

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