We identified a core nucleoporin, Nup84, and an inner nuclear membrane protein, Src1, in M. oryzae.
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Nup84-tdTomato and Src1-EGFP localized within the nuclear envelope of M. oryzae.
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Nup84-tdTomato remained associated with the dividing nucleus.
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Nup84-tdTomato accumulated near presumed spindle pole bodies during mitotic nuclear migration.
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Appressorium development involved a mitotic nuclear migration event through the germ tube.
Abstract
The arrangement of the nuclear envelope in the rice blast fungus, Magnaporthe oryzae, was previously undetermined. Here, we identified two conserved components of the nuclear envelope, a core nucleoporin, Nup84, and an inner nuclear membrane protein, Src1. Live-cell super-resolution structured illumination microscopy revealed that Nup84-tdTomato and Src1-EGFP colocalized within the nuclear envelope during interphase and that Nup84-tdTomato remained associated with the dividing nucleus. We also found that appressorium development involved a mitotic nuclear migration event through the germ tube.
