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Comparative bioefficacy of Bacillus and Pseudomonas chitinase against Helopeltis theivora in tea (Camellia sinensis (L.) O.Kuntze

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Abstract

Tea (Camellia sinensis (L.) O.Kuntze) is an industry-oriented economical crop in India. Among the sap sucking pests, tea mosquito bug (Helopeltis theivora) is one of the most serious pests causing heavy crop loss in tea plantation. Continuous use of chemical pesticides causes environmental pollution and health hazards besides developing pesticide residues in tea powder. The control of pests by bacterial metabolite is an alternative that may contribute to reduce or eliminate the chemical pesticide use. The use of chitinase as a biological control is an emerging field of research. In the present study, Chitinase (~ 25 kDa) was purified from Bacillus cereus C-13 strain using gel-filtration chromatography and further characterized for its optimum pH, temperature and substrate specificity. Bioefficacy of chitinase from B. cereus C-13 was compared with our previously reported Pseudomonas fluorescens MP-13 chitinase against H. theivora. Result concluded that, 100% and 78% mortality was observed by using P. fluorescens MP-13 chitinase and B. cereus C-13 chitinase, respectively. In future, bacterial chitinase can be utilized in eco-friendly pest management strategies.

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Acknowledgments

We gratefully acknowledge the Director, UPASI Tea Research Institute, Valparai, TamilNadu, India for positive encouragements and facilities. First author sincerely acknowledge Dr. K.N. Chandrashekara for his valuable support and suggestions during the study.

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Authors and Affiliations

  1. Department of Plant Physiology and Biotechnology, UPASI Tea Research Institute, Valparai, Tamilnadu, 642 127, India

    M. Suganthi & S. Arvinth

  2. Department of Biotechnology, Vels Institute of Science, Technology and Advanced Studies (VISTAS), Pallavaram, Chennai, Tamilnadu, 600 117, India

    M. Suganthi

  3. Department of Genetic Engineering, School of Bioengineering, SRM Institute of Science and Technology, Potheri, Kattankulathur, Chengalpattu District, Tamilnadu, 603 203, India

    P. Senthilkumar

  4. Department of Botany, PSG College of Arts and Science, Coimbatore, Tamilnadu, 641 004, India

    S. Arvinth

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  1. M. Suganthi
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Correspondence to P. Senthilkumar.

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Suganthi, M., Arvinth, S. & Senthilkumar, P. Comparative bioefficacy of Bacillus and Pseudomonas chitinase against Helopeltis theivora in tea (Camellia sinensis (L.) O.Kuntze. Physiol Mol Biol Plants 26, 2053–2060 (2020). https://doi.org/10.1007/s12298-020-00875-2

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