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Influence of Prenatal Hypoxia on the Content of Neuron Specific Enolase in the Structures of the Brain and Blood Serum of Rats in Early Ontogeny

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Abstract—We studied the dynamics of changes in the content of neuron specific enolase (NSE, EC 4.2.1.11) in the hippocampus, cortex, cerebellum and blood serum of rats at 5th, 10th, and 30th days of postnatal development. It was shown that during the first month of life the content of NSE increases several-fold in all the structures studied, both after hypoxia and in the animals not exposed to hypoxia. It was established that prenatal hypoxia on the 14th day of embryonic development leads to significant changes in the NSE content in all the brain structures studied. In the blood serum, the content of this enzyme is higher in comparison with the control group on the 5th, 10th, and 30th postnatal days in experimental animals. Thus, the lack of oxygen affects the content of this enzyme in both the brain structures and in blood serum of rats, which makes it possible to judge the survival of neurons under conditions of prenatal hypoxia, and also suggests that this index may be used as a marker of fetal CNS disturbance after hypoxia during prenatal development.

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REFERENCES

  1. Dubrovskaya, N.M., Nalivaeva, N.N., Vasilev, D.S., Bagrova, D.I., and Zhuravin, I.A., Mechanisms of short-term working memory deficit, in Short-Term Memory: New Research, Kalivas, S. and Petralia, G.F, Eds., New York: Nova Science Publishers, Inc., 2012, Chapter 6, pp. 155–173.

  2. Morozova, A.Yu., Arutyunyan, A.V., Milyutina, Yu.P., Morozova, P.Yu., Kozina, L.S., and Zhuravin, I.A., Neurochem. J., 2018, vol. 12, no. 3, pp. 262–269.

    Article  CAS  Google Scholar 

  3. Dubrovskaya, N.M. and Zhuravin, I.A., Zhurn. Vysshei nervnoi deyatel’nosti, 2008, vol. 58, no. 6, pp. 718–727.

  4. Isgro, M.A., Bottori, P., and Scatena, R., Adv. Exp. Med. Biol., 2015, vol. 867, pp. 125–143.

    Article  CAS  Google Scholar 

  5. Hague, A., Polcyn, R., Matcelle, D., and Banik, N.L., Brain. Sci., 2018, vol. 8, pp. 33–45.

    Article  Google Scholar 

  6. Blinov, D.V. and Terent’ev, A.A., Neurochem. J., 2013, vol. 7, no. 3, pp. 159–170.

    Article  CAS  Google Scholar 

  7. Rakhimbaeva, G.S. and Rashidova, N.S., Mezhd. Nevrologicheskii Zhurn., 2011, vol. 2, pp. 123–128.

  8. Blennow, K., Wallin, A., and Ekamn, R., J. Neurol.Transm., 1994, vol. 8, pp. 27–30.

    Google Scholar 

  9. Zhukova, I.A., Alifirova, V.M., and Zhukova, N.G., Byulleten’ Sibirskoi Meditsiny, 2011, no. 2, pp. 15–21.

  10. Kilkenny, C., Browne, W.J., Cuthill, I.C., Emerson, M., and Altman, D.G., PLoS Biol., 2010, vol. 8, no. 6.

  11. Bradford, M.M., Anal. Biochem., 1976, vol. 72, pp. 248–254.

  12. Vasil’ev, D.S., Tumanova, N.L., and Zhuravin, I.A., Zhurn. Evolyuts. Biokh. Fiziol, 2008, vol. 44, no. 3, pp. 258–266.

  13. Burke, R.E. and Baimbridge, K.G., J. Neuroscience, 1993, vol. 56, no. 2, pp. 305–315.

    Article  CAS  Google Scholar 

  14. Rees, S. and Indel, T., Early Hum. Dev., 2005, vol. 81, no. 9, pp. 753–761.

    Article  Google Scholar 

  15. Vasilev, D.S., Dubrovskaya, N.M., Tumanova, N.L., and Zhuravin, I.A., Front. Neurosci., 2016, vol. 10, Article 126. https://doi.org/10.3389/fnins.2016.00126

  16. Zhuravin, I.A., Tumanova, N.L., and Vasil’ev, D.S., Dokl. Ross. Akad. Nauk, 2009, vol. 425, pp. 123–125.

    Google Scholar 

  17. Zhuravin, I.A., Dubrovskaya, N.M., and Tumanova, N.L., Ros. Fiziol. Zhurn. im. I.M. Sechenova, 2003, vol. 89, pp. 522–532.

    CAS  Google Scholar 

  18. Reznikov, K.Yu., Proliferatsiya kletok mozga pozvonochnykh v usloviyakh normal’nogo razvitiya mozga i pri ego travme (Proliferation of Brain Cells of Vertebrates under Conditions of Normal Development and after Brain Trauma), Moscow: Nauka, 1981.

  19. Miller, M.W., J. Comp. Neurol., 1989, vol. 287, pp. 326–338.

    Article  CAS  Google Scholar 

  20. Eshchenko, N.D., Putilina, F.E., and Galkina, O.V., in Biokhimiya razvivayushchegosya mozga. Izbrannye razdely (Biochemistry of Developing Brain. Selected Chapters), St. Petersburg: Izd-vo SPbU, 2013.

  21. Kaushanskaya, E.Ya., Extended Abstract of Cand. Sci. (Med.) Dissertation, 1993.

  22. Chekhonin, V.P., Dmitrieva, T.B., and Zhirkov, Yu.A., Immunokhimicheskii analiz neirospetsificheskikh anti-genov (Immunochemical Analysis of Neuron-Specific Antigens), Moscow, 2000.

  23. Marangos, P.J., Schmechel, D.E., Parma, A.M., and Goodwin, F.K., Brain Res., 1980, vol. 3, pp. 185–193.

    Article  Google Scholar 

  24. Schaf, D.V., Tort, A.B., Fricke, D., Schestatsky, P., Portela, L.V., Souza, D.O., and Rieder, C.R., Parkinsonism Relat. Disord., 2005, vol. 11, pp. 39–43.

    Article  Google Scholar 

  25. Chaves, M.L., Cammozzato, A.L., Ferreira, E.D., Piazenski, I., Kochhann, R., Dalligna, O., Mazzini, G.S., Souza, D.O., and Portela, L.V., J. Neuroinflam., 2010, vol. 7, pp. 6–18.

    Article  Google Scholar 

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Funding

The work was performed as a part of the state tasks AAAA-A18-118012290373-7 and AAAA-A19-1190212901-1

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Authors and Affiliations

  1. Ott Institute of Obstetrics, Gynecology, and Reproductology, Mendeleevskaya liniya 3, 199034, St. Petersburg, Russia

    A. Yu. Morozova, A. V. Arutjunyan & Yu. P. Milyutina

  2. Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St. Petersburg, Russia

    I. A. Zhuravin

  3. St. Petersburg State University, St. Petersburg, Russia

    P. Yu. Morozova

  4. St. Petersburg Institute of Bioregulation and Gerontology, St. Petersburg, Russia

    A. V. Arutjunyan & L. S. Kozina

Authors
  1. A. Yu. Morozova
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  2. A. V. Arutjunyan
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  3. Yu. P. Milyutina
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  4. P. Yu. Morozova
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  5. L. S. Kozina
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  6. I. A. Zhuravin
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Correspondence to A. V. Arutjunyan.

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Conflict of interest. The authors declared no conflicts of interest.

Ethical approval. All procedures for the care and use of animals were performed in accordance with the requirements of the Ethics Committees of the Ott Research Institute of Obstetrics, Gynecology and Reproductology and the Sechenov Institute of Evolutionary Physiology and Biochemistry, European Communities Council Directive 1986 (86/609 / EEC) and “Guide for the Care and Use of Laboratory Animals.”

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Morozova, A.Y., Arutjunyan, A.V., Milyutina, Y.P. et al. Influence of Prenatal Hypoxia on the Content of Neuron Specific Enolase in the Structures of the Brain and Blood Serum of Rats in Early Ontogeny. Neurochem. J. 14, 290–294 (2020). https://doi.org/10.1134/S1819712420030083

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