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Polymorphism and Phylogeny of the Vacuolar Invertase Inhibitor Gene INH2 Homologs in Solanaceae Species

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Abstract

INH2 homologous genes encoding vacuolar invertase inhibitor were identified in 37 Solanaceae species, and the INH2 polymorphism was evaluated. A significant level of interspecific polymorphism of the INH2 coding sequence was found within the Petota and Lycopersicon sections, which corresponds to the high mutation rate during the evolution of the INH2 genes and presumably associated with the modern plant diversity. Structural analysis revealed the presence of conserved motifs and amino acid residues, non-transmembrane signal peptide and the PMEI domain in the INH2 proteins. The possibility of applying the INH2 sequence to assess the inter-genera and inter-section Solanaceae phylogeny was shown.

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ACKNOWLEDGMENTS

The authors are grateful to S.R. Mayorov for providing plant material, as well as E.O. Shmelkova for significant technical assistance in conducting the study.

Funding

This work was supported by the Russian Foundation for Basic Research (grant no. 18-016-00108), the Ministry of Science and Higher Education of the Russian Federation, and the Federal Targeted Program for Agricultural Development of the Russian Federation for 2017–2025 (subprogram Development of Selection and Seed Production of Potatoes in the Russian Federation).

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Authors and Affiliations

  1. Institute of Bioengineering, Research Center of Biotechnology, Russian Academy of Sciences, 119071, Moscow, Russia

    M. A. Slugina, E. Z. Kochieva & A. V. Shchennikova

  2. Moscow State University, 119991, Moscow, Russia

    E. Z. Kochieva

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  1. M. A. Slugina
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  2. E. Z. Kochieva
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  3. A. V. Shchennikova
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Correspondence to M. A. Slugina.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Slugina, M.A., Kochieva, E.Z. & Shchennikova, A.V. Polymorphism and Phylogeny of the Vacuolar Invertase Inhibitor Gene INH2 Homologs in Solanaceae Species. Biol Bull Russ Acad Sci 47, 474–481 (2020). https://doi.org/10.1134/S1062359020040111

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