Skip to main content
SpringerLink
Log in

A Study of Protein Fractional Composition during Incubation of Diphyllobothrium dendriticum (Cestoda) Plerocercoids in a Medium Containing Blood Serum of the Host, the Baikal Omul Coregonus migratorius (Coregonidae)

  • ZOOLOGY
  • Published:
Biology Bulletin Aims and scope Submit manuscript

Abstract

The change in the composition of the protein fraction of Diphyllobothrium dendriticum (Cestoda) plerocercoids during incubation in a medium containing blood serum of their host—the Baikal omul Coregonus migratorius (Coregonidae)—was investigated. The change in the composition of the protein fraction of the incubation medium also was studied. On the one hand, the appearance of new high-molecular fractions with a molecular weight (MW) of 193 kDa after 3, 12, and 24 h of incubation and 88 kDa after 24 h was detected, which was associated at plerocercoids with the appearance of a new 189 kDa fraction after 12 h and the disappearance of the 80 kDa fraction. On the other hand, a decrease or the disappearance of two low-molecular fractions 57 and 42 kDa in the incubation medium after 3, 12, and 24 h of incubation were observed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.
Fig. 3.
Fig. 4.

Similar content being viewed by others

REFERENCES

  1. Andreeva, A.M., Structural and functional organization of proteins of the blood and some other extracellular fluids of fish, Extended Abstract of Doctoral (Biol.) Dissertation, Moscow: MGU, 2008.

  2. Berezantsev, Yu.A., Suppression of the inflammatory cellular reaction by helminth larvae and the specificity of their encapsulation in host tissues, Dokl. Akad. Nauk SSSR, 1975, vol. 220, no. 1, pp. 227–229.

    PubMed  Google Scholar 

  3. Berezantsev, Yu.A., Borshchukov, D.V., Oksov, I.V., and Chesnokova, M.V., Encapsulation of larvae of parasitic nematodes and cestodes in vertebral tissues as a form of the relationship between the parasite and the host, in Parazitologicheskii sbornik ZIN AN SSSR (Collection of Parasitological Articles of the Zoological Institute of the Academy of Sciences of the USSR), Leningrad: Nauka, 1989, vol. 36, pp. 131–160.

  4. Biserova, N.M. and Kutyrev, I.A., Localization of prostaglandin E2, γ-aminobutyric acid, and other potential immunomodulators in the plerocercoid Diphyllobothrium dendriticum (Cestoda), Biol. Bull. (Moscow), 2014, vol. 41, no. 3, pp. 242–250.

    Article  CAS  Google Scholar 

  5. Biserova, N.M., Kutyrev, I.A., and Malakhov, V.V., The tapeworm Diphyllobothrium dendriticum (Cestoda) produces prostaglandin E2, a regulator of host immunity, Dokl. Biol. Sci., 2011, vol. 441, pp. 367–369.

    Article  CAS  PubMed  Google Scholar 

  6. Biserova, N.M., Kutyrev, I.A., and Jensen, K., GABA in the nervous system of the cestodes Diphyllobothrium dendriticum (Diphyllobothriidea) and Caryophyllaeus laticeps (Caryophyllidea), with comparative analysis of muscle innervation, J. Parasitol., 2014, vol. 100, no. 4, pp. 411–421.

    Article  CAS  PubMed  Google Scholar 

  7. Buck, A.H., Coakley, G., Simbari, F., McSorley, H.J., Quintana, J.F., Le Bihan, T., Kumar, S., Abreu-Goodger, C., Lear, M., Harcus, Y., Ceroni, A., Babayan, S.A., Blaxter, M., Ivens, A., and Maizels, R.M., Exosomes secreted by nematode parasites transfer small RNAs to mammalian cells and modulate innate immunity, Nat. Commun., 2014, vol. 25, no. 5, pp. 54–88.

    Google Scholar 

  8. Bulgheresi, S., Schabussova, I., Chen, T., Mullin, N.P., Maizels, R.M., and Ott, J.A., A new C-type lectin similar to the human immunoreceptor DC-SIGN mediates symbiont acquisition by a marine nematode, Appl. Environ. Microbiol., 2006, vol. 72, pp. 2950–2956.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Chiumiento, L. and Bruschi, F., Enzymatic antioxidant systems in helminth parasites, Parasitol. Res., 2009, vol. 105, pp. 593–603.

    Article  PubMed  Google Scholar 

  10. Cho, Y., Structural and functional characterization of a secreted hookworm macrophage migration inhibitory factor (MIF) that interacts with the human MIF receptor CD74, J. Biol. Chem., 2007, vol. 282, no. 32, pp. 23447–23456.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Coakley, G., Maizels, R.M., and Buck, A.H., Exosomes and other extracellular vesicles: the new communicators in parasite infections, Trends Parasitol., 2015, vol. 31, pp. 477–489.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Coakley, G., Buck, A.H., and Maizels, R.M., Host parasite communications—messages from helminths for the immune system: parasite communication and cell–cell interactions, Mol. Biochem. Parasitol., 2016, vol. 208, pp. 33–40.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Davydov, V.G. and Mikryakov, V.R., Adaptive structures of the integument of some cestodes associated with the protection of parasites from the effects of the host organism, in Immunologicheskie i biokhimicheskie aspekty vzaimootnoshenii gel’minta i khozyaina (Immunological and Biochemical Aspects of the Relationship of Helminth and Host), Moscow: Nauka, 1988, pp. 88–100.

  14. Finlay, C.M., Walsh, K.P., and Mills, K.H., Induction of regulatory cells by helminth parasites: exploitation for the treatment of inflammatory diseases, Immunol. Rev., 2014, vol. 259, no. 1, pp. 206–230.

    Article  CAS  PubMed  Google Scholar 

  15. Gregory, W.F. and Maizels, R.M., Cystatins from filarial parasites: evolution, adaptation and function in the host-parasite relationship, Int. J. Biochem. Cell. Biol., 2008, vol. 40, pp. 1389–1398.

    Article  CAS  PubMed  Google Scholar 

  16. Harnett, W. and Harnett, M.M., Therapeutic immunomodulators from nematode parasites, Expert. Rev. Mol. Med., 2008, vol. 10, pp. 7–18.

    Article  Google Scholar 

  17. Hewitson, J.P., Grainger, J.R., and Maizels, R.M., Helminth immunoregulation: the role of parasite secreted proteins in modulating host immunity, Mol. Biochem. Parasitol., 2009, vol. 167, pp. 1–11.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Informatsionnyi sbornik statisticheskikh i analiticheskikh materialov (Information Collection of Statistical and Analytical Materials), section 3.2: Zabolevaemost’ protozoozami i gel’mintozami naseleniya Rossiiskoi Federatsii v 1996–2000 godakh (The Incidence of Protozoan and Helminth Infections in the Population of the Russian Federation in 1996–2000), Moscow: Federal’nyi tsentr Gossanepidnadzora Minzdrava Rossii, 2001.

  19. Informatsionnyi sbornik statisticheskikh i analiticheskikh materialov (Information Collection of Statistical and Analytical Materials), section 3.2: Zabolevaemost’ protozoozami i gel’mintozami naseleniya Rossiiskoi Federatsii v 2008-2009 gg. (The Incidence of Protozoan and Helminth Infections in the Population of the Russian Federation in 2008–2009), Moscow: Federal’nyi tsentr Gossanepidnadzora Minzdrava Rossii, 2010.

  20. Informatsionnyi sbornik statisticheskikh i analiticheskikh materialov (Information Collection of Statistical and Analytical Materials), section 3.2: Zabolevaemost’ protozoozami i gel’mintozami naseleniya Rossiiskoi Federatsii v2012–2013 gg. (The Incidence of Protozoan and Helminth Infections in the Population of the Russian Federation in 2012–2013), Moscow: Federal’nyi tsentr Gossanepidnadzora Minzdrava Rossii, 2014.

  21. Izvekova, G.I. and Korneva, Zh.V., Localization of the processes of symbiotic digestion on the digestive and transport structures of fishes and cestodes, Usp. Sovrem. Biol., 2007, vol. 127, no. 5, pp. 502–513.

    Google Scholar 

  22. Jenkins, S.J., Hewitson, J.P., Jenkins, G.R., and Mountford, A.P., Modulation of the host’s immune response by Schistosome larvae, Parasitol. Immunol., 2005, vol. 27, pp. 385–393.

    Article  CAS  Google Scholar 

  23. Knopf, K., Naser, K., van der Heijden, M.H.T., and Taraschewski, H., Humoral immune response of European eel Anguilla anguilla experimentally infected with Anguillicola crassus,Dis. Aquat. Org., 2000, vol. 42, pp. 61–69.

    Article  CAS  PubMed  Google Scholar 

  24. Krylov, Yu.M., Malenev, A.L., Sokolov, E.E., and Krasnoshchekov, G.P., Secretory proteins of plerocercoids of the cestode Digramma interrupta and their enzymatic activity, Parazitologiya, 1991, vol. 25, no. 3, pp. 278–280.

    Google Scholar 

  25. Kuklina, M.M. and Kuklin, V.V., Diphyllobothrium dendriticum (Cestoda: Diphyllobothriidae) in the intestinal tract of the herring gull Larus argentatus: localization and trophic parameters, Biol. Bull. (Moscow), 2016, vol. 42, no. 4, pp. 329–334.

    Article  Google Scholar 

  26. Kuperman, B.I., Funktsional’naya morfologiya nizshikh tsestod: ontogeneticheskii i evolyutsionnyi aspekty (Functional Morphology of Lower Cestodes: Ontogenetic and Evolutionary Aspects), Leningrad: Nauka, 1988.

  27. Kutyrev, I.A., Morphofunctional and immunological aspects of the relationships in the parasite–host system: cestodes–fish, Extended Abstract of Doctoral (Biol.) Dissertation, Moscow: Inst. Probl. Ekol. Evol., Ross. Akad. Nauk, 2017.

  28. Kutyrev, I.A., Franke, F., Büscher, J., Kurtz, J., and Scharsack, J.P., In vitro effects of prostaglandin E2 on leucocytes from sticklebacks (Gasterosteus aculeatus) infected and not infected with the cestode Schistocephalus solidus,Fish Shellfish Immunol., 2014, vol. 41, pp. 473–481.

    Article  CAS  PubMed  Google Scholar 

  29. Kutyrev, I.A., Biserova, N.M., Olennikov, D.N., Korneva, J.V., and Mazur, O.E., Prostaglandins E2 and D2—regulators of host immunity in the model parasite diphyllobothrium dendriticum: an immunocytochemical and biochemical study, Mol. Biochem. Parasitol., 2017, vol. 212, pp. 33–45.

    Article  CAS  PubMed  Google Scholar 

  30. L’vova, M.N., Duzhak, T.G., Tsentalovich, Yu.P., Katokhin, A.V., and Mordvinov, V.A, The secretome of the hepatic trematode Opisthorchis felineus marita, Parazitologiya, 2014, vol. 48, no. 3, pp. 169–184.

    Google Scholar 

  31. Li, J., Shao, J., Xiang, L., and Wen, Y., Cloning, characterization and expression analysis of pufferfish interleukin-4 cDNA: the first evidence of Th2-type cytokine in fish, Mol. Immunol., 2007, vol. 44, pp. 2078–2086.

    Article  CAS  PubMed  Google Scholar 

  32. Lightowlers, M.W. and Rickard, M.D., Excretory-secretory products of helminth parasites: effects on host immune responses, Parasitology, 1988, vol. 96, suppl. pp. S123–S166.

    Article  PubMed  Google Scholar 

  33. Maizels, R.M., Balic, A., Gomez-Escobar, N., Nair, M., Taylor, M.D., and Allen, J.E., Helminth parasites—masters of regulation, Immun. Rev., 2004, vol. 201, pp. 89–116.

    Article  CAS  PubMed  Google Scholar 

  34. Marcilla, A., Trelis, M., Cortés, A., Sotillo, J., Cantalapiedra, F., Minguez, M.T., Valero, M.L., Sánchez del Pino, M.M., Muñoz-Antoli, C., Toledo, R., and Bernal, D., Extracellular vesicles from parasitic helminths contain specific excretory/secretory proteins and are internalized in intestinal host cells, PLoS One, 2012, vol. 7, no. 9, pp. 459–474.

    Article  Google Scholar 

  35. Mazur, O.E. and Tolochko, L.V., Cytomorphological and biochemical characteristics of the whitefish, Baikal omul Coregonus migratorius, infected by plerocercoids of Diphyllobothrium dendriticum (Cestoda: Pseudophyllidae) Biol. Bull. (Moscow), 2015, vol. 42, no. 2, pp. 117–123.

    Article  CAS  Google Scholar 

  36. McSorley, H.J. and Maizels, R.M., Helminth infections and host immune regulation, Clin. Microbiol. Rev., 2012, vol. 25, no. 4, pp. 585–608.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  37. Pleass, R.J., Kusel, J.R., and Woof, J.M., Cleavage of human IgE mediated by Schistosoma mansoni,Int. Arch. Allergy Immunol., 2000, vol. 121, pp. 194–204.

    Article  CAS  PubMed  Google Scholar 

  38. Poulin, R. and Morand, S., The diversity of parasites, Quart. Rev. Biol., 2000, vol. 75, pp. 277–293.

    Article  CAS  PubMed  Google Scholar 

  39. Pronin, N.M., Pronina, S.V., and Kutyrev, I.A., The structure of the Baikal natural focus of diphyllobothriasis and the relationships of Diphyllobothrium dendriticum with definitive hosts, Izv. Irkut. Gos. Univ., Ser. Biol. Ekol., 2009, no. 1, pp. 53–56.

  40. Serdyukov, A.M., Difillobotriidy Zapadnoi Sibiri (Difillobotriids of Western Siberia), Novosibirsk: Nauka, 1979.

  41. Victor, B., Kanobana, K., Gabriël, S., Polman, K., Deckers, N., Dorny, P., Deelder, A.M., and Palmblad, M., Proteomic analysis of Taenia solium metacestode excretion–secretion proteins, Proteomics, 2012, vol. 11, pp. 1860–1869.

    Article  Google Scholar 

  42. Wang, T., Husain, M., Hong, S., and Holland, J.W., Differential expression, modulation and bioactivity of distinct fish IL-12 isoforms: implication towards the evolution of Th1-like immune responses, Eur. J. Immunol., 2014, vol. 44, pp. 1541–1551.

    Article  CAS  PubMed  Google Scholar 

  43. Wang, T., Johansson, P., Abos, B., Holt, A., Tafalla, C., Jiang, Y., Wang, A., Xu, Q., Qi, Z., Huang, W., Costa, M.M., Diaz-Rosales, P., Holland, J.W., and Secombes, C.J., First in-depth analysis of the novel Th2-type cytokines in salmonid fish reveals distinct patterns of expression and modulation but overlapping bioactivities, Oncotarget, 2016, vol. 10, pp. 10917–10946.

    Article  Google Scholar 

Download references

ACKNOWLEDGMENTS

The authors are grateful to A.V. Bazov (Baikal Branch of the State Fishery Center) for assistance in collecting material. Sample preparation, electrophoresis, and visualization of proteins were performed on the basis of the Proteomic Analysis Center of the Scientific Research Institute of Biomedical Physics and Technology.

Funding

This study was carried under a topic of a state assignment (state registration number AAAA-A17-117011810039-4) and was supported by the Russian Foundation for Basic Research (project no. 19-04-00666).

Author information

Authors and Affiliations

  1. Institute of General and Experimental Biology, Siberian Branch, Russian Academy of Sciences, 670047, Ulan-Ude, Russia

    I. A. Kutyrev & O. E. Mazur

  2. Institute of Molecular Biology and Biophysics, Federal Research Center of Fundamental and Translational Medicine, 630117, Novosibirsk, Russia

    O. B. Goreva

  3. Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, 630090, Novosibirsk, Russia

    V. A. Mordvinov

Authors
  1. I. A. Kutyrev
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. O. B. Goreva
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. O. E. Mazur
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. V. A. Mordvinov
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to I. A. Kutyrev.

Ethics declarations

Conflict of interest. The authors declare that they have no conflict of interest.

Statement on the welfare of animals. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kutyrev, I.A., Goreva, O.B., Mazur, O.E. et al. A Study of Protein Fractional Composition during Incubation of Diphyllobothrium dendriticum (Cestoda) Plerocercoids in a Medium Containing Blood Serum of the Host, the Baikal Omul Coregonus migratorius (Coregonidae). Biol Bull Russ Acad Sci 47, 490–496 (2020). https://doi.org/10.1134/S1062359020040081

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1062359020040081

Search

Navigation