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Enhancement of Galactose Uptake from Kappaphycus alvarezii Hydrolysate Using Saccharomyces cerevisiae Through Overexpression of Leloir Pathway Genes

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Abstract

A total 42.68 g/L monosaccharide with 0.10 g/L HMF was obtained from 10% (w/v) Kappaphycus alvarezii with thermal acid hydrolysis using 350 mM HNO3 at 121 °C for 60 min and enzymatic saccharification with a 1:1 mixture of Viscozyme L and Celluclast 1.5 L for 72 h. To enhance the galactose utilization rate, fermentation was performed with overexpression of GAL1 (galactokinase), GAL7 (galactose-1-phosphate uridyltransferase), GAL10 (UDP-glucose-4-epimerase), and PGM2 (phosphoglucomutase 2) in Saccharomyces cerevisiae CEN.PK2 using CCW12 as a strong promoter. Among the strains, the overexpression of PGM2 showed twofold high galactose utilization rate (URgal) and produced ethanol 1.4-fold more than that of the control. Transcriptional analysis revealed the increase of PGM2 transcription level leading to enhance glucose-6-phosphate and fructose-6-phosphate and plays a key role in ensuring a higher glycolytic flux in the PGM2 strain. This finding shows particular importance in biofuel production from seaweed because galactose is one of the major monosaccharides in seaweeds such as K. alvarezii.

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Funding

This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2019R1F1A1041288).

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Author notes

  1. In Yung Sunwoo and Pailin Sukwong contributed equally to this work.

Authors and Affiliations

  1. Division of Marine, Fisheries, and Life Science, Pukyong National University, Busan, 48513, South Korea

    In Yung Sunwoo, Pailin Sukwong, Yu Rim Park, Gwi-Teak Jeong & Sung-Koo Kim

  2. Department of Chemistry, Umeå University, 90187, Umeå, Sweden

    In Yung Sunwoo

  3. School of Food Science and Biotechnology, Kyungpook National University, Daegu, 41566, South Korea

    Deok Yeol Jeong & Soo Rin Kim

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  1. In Yung Sunwoo
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  2. Pailin Sukwong
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Sunwoo, I.Y., Sukwong, P., Park, Y.R. et al. Enhancement of Galactose Uptake from Kappaphycus alvarezii Hydrolysate Using Saccharomyces cerevisiae Through Overexpression of Leloir Pathway Genes. Appl Biochem Biotechnol 193, 335–348 (2021). https://doi.org/10.1007/s12010-020-03422-7

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  • DOI: https://doi.org/10.1007/s12010-020-03422-7

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