Abstract
Immobilization of the Geotrichum candidum (CCT 1205) cell with functionalized silica creates promising biocatalysts for production of ɛ-caprolactone. The results obtained by immobilization of the whole cell on SiO2-NH2 and SiO2-SH supports indicate that the presence of reactive functional groups on the support may promote effective chemical bonds with the cell walls resulting the decreased dehydrogenases enzyme activity (5% yield in less than 2h) and consequently, increased Baeyer-Villiger monooxygenases enzyme activity with redacting of 25% of time reaction when is used SiO2-NH2 as support and 50% through use of SiO2-SH as support relative to free cells when cyclohexanone is used as a substrate. The catalysts SiO2–NH2–Geotrichum candidum and SiO2–SH–Geotrichum candidum were recycling and reused in the ɛ-caprolactone synthesis from cyclohexanone, and the biocatalysts promoted a quantitative conversion up to the eighth reaction cycle.
Key points
• Immobilized microorganism is more efficient than free cell in the caprolactone synthesis.
• The reaction times for amino and thiol groups in support were 3 h and 2 h, respectively.
• These catalysts showed higher ɛ-caprolactone conversion at higher concentrations.
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Acknowledgements
We would like to acknowledge the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) , Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Centro de Tecnologias Estratégicas do Nordeste (CETENE) for their financial support.
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This work was supported by the CNPQ (No. 304403/2017-2).
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Juliana A. Vale conceived and designed research and the other authors conducted experiments.
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Silva, A.L.P., da Silva Caridade, T.N., Magalhães, R.R. et al. Biocatalytic production of Ɛ-caprolactone using Geotrichum candidum cells immobilized on functionalized silica. Appl Microbiol Biotechnol 104, 8887–8895 (2020). https://doi.org/10.1007/s00253-020-10875-7
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DOI: https://doi.org/10.1007/s00253-020-10875-7