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The gut bacterial flora associated with brown planthopper is affected by host rice varieties

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Abstract

Gut microbiota plays vital roles in the development, evolution and environmental adaptation of the host insects. The brown planthopper (BPH) is one of the most destructive pests of rice, but little is known about its gut microbiota. In this study, we investigated the gut bacterial communities in two BPH populations feeding on susceptible and resistant rice varieties by high-throughput amplicon sequencing. Our results revealed that the gut bacterial communities in BPH were species diverse. A total of 29 phyla and 367 genera were captured, with Proteobacteria and Acinetobacter being the most prominent phylum and genus, respectively. Comparative analysis showed that significant differences in the profile of gut bacterial communities existed between the two BPH populations. The species richness detected in the population feeding on the resistant rice variety was significantly higher than that in the population rearing on the susceptible rice variety. Although the most dominant gut bacteria at all taxonomic levels showed no significant differences between the two BPH populations, the relative abundances of two subdominant phyla (Firmicutes and Bacteroidetes) and two subdominant classes (Bacteroidia and Clostridia) were significantly different. FAPROTAX analysis further indicated that host rice varieties might induce changes of the gut bacterial flora in BPH, as significant differences in five metabolism-related functional categories (fermentation, methylotrophy, xylanolysis, nitrate reduction and ureolysis) were detected between the two BPH populations. Our results are informative for studies which focused on the interactions between BPH and its symbiotic microbes and could also provide the basis of future BPH biological management.

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Acknowledgements

This work was financially supported by the Natural Science Foundation of Zhejiang Province (Grant No. LR19C140001), the National Natural Science Foundation of China (Grant no. 31972347; 31601698) and the Key Research and Development Program of Zhejiang Province (Grant No. 2019C02015).

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  1. Zheng-Liang Wang and Hai-bo Pan these two authors contributed equally to this paper.

Authors and Affiliations

  1. Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, College of Life Sciences, China Jiliang University, Hangzhou, 310018, Zhejiang, People’s Republic of China

    Zheng-Liang Wang, Hai-bo Pan, Wei Wu, Mu-Yu Li & Xiao-Ping Yu

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203_2020_2013_MOESM1_ESM.rar

Supplementary file1 (RAR 283 kb) Table S1 Shared and unique bacterial OTUs in the gut of two BPH populations rearing on a susceptible rice variety (TN1) and a resistance rice variety (ASD7), respectively.Table S2 Function classification of bacterial community in the gut of BPH rearing on a susceptible rice variety (TN1) and a resistance rice variety (ASD7) by FAPROTAX.Fig. S1. Rarefaction curves of bacterial 16S rRNA sequences in BPH rearing on a susceptible rice variety (TN1) and a resistance rice variety (ASD7).

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Wang, ZL., Pan, Hb., Wu, W. et al. The gut bacterial flora associated with brown planthopper is affected by host rice varieties. Arch Microbiol 203, 325–333 (2021). https://doi.org/10.1007/s00203-020-02013-8

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