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Activities of Succinate Dehydrogenase and Lactate Dehydrogenase in Blood Lymphocytes in Yakut Ground Squirrels Spermophilus undulatus During Hibernation and in the Active State

  • BIOPHYSICS AND BIOCHEMISTRY
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We studied energy metabolism in blood lymphocytes of Yakut ground squirrels Spermophilus undulatus in active state and during hibernation. Activities of succinate dehydrogenase (SDH) and lactate dehydrogenase (LDH), marker enzymes of mitochondrial respiration and glycolysis, were measured by a cytobiochemical method based on quantitative assessment of a product of NBT reduction to diformazan in blood lymphocytes immobilized on glass. To measure SDH and LDH activities, cytobiochemical staining of immobilized cells was performed with succinate, lactate, and NAD. In the state of hibernation, SDH activity decreased by 3 times and LDH activity decreased by 10 times or more. These results suggest that the decrease in metabolic activity in lymphocytes of ground squirrels during hypothermia is associated with inhibition of glycolysis, rather than with mitochondrial energy supply.

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Authors and Affiliations

  1. Institute of Theoretical and Experimental Biophysics, Russian Academy of Science, Moscow, Russia

    N. V. Khunderyakova

  2. Institute of Cell Biophysics, Russian Academy of Science — Separate Division of Federal Research Center Pushchino Scientific Center for Biological Research, Russian Academy of Sciences, Pushchino, Russia

    N. M. Zakharova

Authors
  1. N. V. Khunderyakova
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  2. N. M. Zakharova
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Correspondence to N. V. Khunderyakova.

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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 169, No. 4, pp. 426-430, April, 2020

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Khunderyakova, N.V., Zakharova, N.M. Activities of Succinate Dehydrogenase and Lactate Dehydrogenase in Blood Lymphocytes in Yakut Ground Squirrels Spermophilus undulatus During Hibernation and in the Active State. Bull Exp Biol Med 169, 445–449 (2020). https://doi.org/10.1007/s10517-020-04906-6

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  • DOI: https://doi.org/10.1007/s10517-020-04906-6

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