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Expression of Nestin and Glial Fibrillary Acidic Protein in the Marginal Ischemic Zone of the Brain in SHR Rats

  • Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
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Bulletin of Experimental Biology and Medicine Aims and scope

We studied spatial organization and structural characteristics of striatal glial cells in spontaneously hypertensive rats (SHR) in 48 h after 30-min focal ischemia. Immunocytochemical analysis of nestin and glial fibrillar acidic protein (GFAP) revealed 3 types of activated astrocytes: expressing only nestin, only GFAP, or both markers. There were no nestin-immunopositive astrocytes in the striatum of sham-operated rats. In cells expressing nestin and GFAP, localization of these markers did not completely coincide, which can be explained by different functions of these proteins or formation of heterodimers of nestin with other intermediate filament proteins.

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Authors and Affiliations

  1. Department of General and Local Morphology, Laboratory of Functional Morphology of Central and Peripheral Nervous System, Institute of Experimental Medicine, St. Petersburg, Russia

    D. L. Tsyba, O. V. Kirik & D. E. Korzhevskii

  2. Department if Pathophysiology, I. P. Pavlov First St. Petersburg State Medical University, Ministry of Health of the Russian Federation, St. Petersburg, Russia

    M. E. Kolpakova & A. A. Yakovleva

Authors
  1. D. L. Tsyba
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  2. O. V. Kirik
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  3. M. E. Kolpakova
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  4. A. A. Yakovleva
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  5. D. E. Korzhevskii
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Correspondence to D. L. Tsyba.

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Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 2, pp. 118-124, June, 2020

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Tsyba, D.L., Kirik, O.V., Kolpakova, M.E. et al. Expression of Nestin and Glial Fibrillary Acidic Protein in the Marginal Ischemic Zone of the Brain in SHR Rats. Bull Exp Biol Med 169, 576–581 (2020). https://doi.org/10.1007/s10517-020-04932-4

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  • DOI: https://doi.org/10.1007/s10517-020-04932-4

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