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Evaluation of the Effects of Aging on the Aorta Stiffness in Relation with Mineral and Trace Element Levels: an Optimized Method via Custom-Built Stretcher Device

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Abstract

Aortic stiffness represents the major cause of aging and tightly associated with hypertension, atherosclerosis, cardiovascular diseases, and increased mortality. Mechanical characteristics of the aorta play a vital role in the blood flow, circulation, systolic pressure, and aortic stiffness; however, the correlation of trace element and mineral levels with aortic stiffness has not been studied before. Balance in the trace elements and minerals is vital for the biological functions; however, natural aging may alter this balance. Thus, after measuring aortic stiffness of aged and young rat aortas by a custom-built stretcher device, trace element and mineral levels were evaluated via ICP-MS. Also, biomarkers of aging including blood pressure, arterial pressure glucose, insulin levels, and histochemical parameters were investigated as well. Aortic stiffness, blood glucose, plasma insulin, systolic, diastolic, and mean arterial pressure significantly increased by aging in the aorta of aged rats compared with the young ones. Also, Fe, Al, Co, Ni, Zn, Sr, Na, Mg, and K levels increased in the aged aorta samples compared with the young aorta samples of rats. Increased levels of the indicated elements may be correlated with the development and progression of aortic stiffness and vascular complications. Thus, possible mechanisms correlating aortic stiffness with the imbalance in the trace element and mineral levels should be further investigated.

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Acknowledgments

The authors gratefully acknowledge the use of the services and facilities of the Koç University Research Center for Translational Medicine (KUTTAM). The content is solely the responsibility of the authors and does not necessarily represent the official views of the Presidency of Strategy and Budget.

Funding

This study was funded by the Presidency of Turkey, Presidency of Strategy, and Budget.

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Authors and Affiliations

  1. School of Medicine, Department of Medical Biochemistry, Koç University, Sariyer, 34450, Istanbul, Turkey

    Duygu Aydemir & Nuriye Nuray Ulusu

  2. Koç University Research Center for Translational Medicine (KUTTAM), Sariyer, 34450, Istanbul, Turkey

    Duygu Aydemir & Nuriye Nuray Ulusu

  3. Department of Mechanical Engineering, Koç University, Rumelifeneri Yolu, Sariyer, 34450, Istanbul, Turkey

    Naveed Salman, Mehrdad Karimzadehkhouei & B. Erdem Alaca

  4. Surface Science and Technology Center (KUYTAM), Koç University, Rumelifeneri Yolu, Sariyer, 34450, Istanbul, Turkey

    B. Erdem Alaca

  5. Faculty of Medicine, Department of Biophysics, Ankara University, 06100, Ankara, Turkey

    Belma Turan

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  1. Duygu Aydemir
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Correspondence to Nuriye Nuray Ulusu.

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All experimental procedures were performed according to the European Community guidelines on the care and use of laboratory animals as described by Olgar et al. [30]. Our study has been approved by the Ankara University with a reference number of 2016-18-165.

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Aydemir, D., Salman, N., Karimzadehkhouei, M. et al. Evaluation of the Effects of Aging on the Aorta Stiffness in Relation with Mineral and Trace Element Levels: an Optimized Method via Custom-Built Stretcher Device. Biol Trace Elem Res 199, 2644–2652 (2021). https://doi.org/10.1007/s12011-020-02380-9

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