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Actinoplanes solisilvae sp. nov., Isolated from Birch Forest Soil

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Abstract

A novel actinomycete, designated strain LAM7112T, was isolated from soil sample collected from a birch forest in Xinjiang Uygur Autonomous Region, China. The new isolate was found to be able to grow at 20–45 °C (optimum: 35 °C), pH 5.0–10.0 (optimum: 7.0) and in the presence of 0–10.0% (optimum: 3.0%) (w/v) NaCl. The isolate formed very scantily irregular sporangia containing motile spores on the substrate mycelium. Phylogenetic analysis of 16S rRNA gene sequences indicated that the new isolate was closely related to members of the family Micromonosporaceae, with highest similarites to Actinoplanes ferrugineus X-14695T (97.4%), Micromonospora zamorensis DSM 45600T (97.3%) and Micromonospora aurantiaca ATCC 27029T (97.3%). In the phylogenetic trees, strain LAM7112T formed a stable phylogenetic subclade within the genus Actinoplanes. The genomic DNA G + C content was 70.0 mol%. The major fatty acids (> 10%) were determined to be iso-C16:0, anteiso-C15:0 and anteiso-C17:0. The predominant menaquinones were identified as MK-9 (H2), MK-9 (H4) and MK-9 (H6). The major polar lipids were found to be diphosphatidylglycerol, phosphatidylinositol and phosphatidylethanolamine. The diagnostic amino acid of the cell wall peptidoglycan was determined to be meso-diaminopimelic acid. The diagnostic sugars in cell hydrolysates were determined to be glucose and ribose. On the basis of its phenotypic, phylogenetic and chemotaxonomic characteristics, strain LAM7112T (= CGMCC 4.7580T = JCM 32512T) is proposed to represent the type strain of a novel species of the genus Actinoplanes, for which the name Actinoplanes solisilvae is proposed.

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Author notes

  1. Qingyun Ma and Qi Zhang have contributed equally to this study and share first authorship.

Authors and Affiliations

  1. Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    Qingyun Ma, Qi Zhang, Xu Jiang, Delong Kong, Xiaoyan Han, Yiqing Zhou & Zhiyong Ruan

  2. College of Life Science, Xinjiang Normal University, Ürümqi, 830046, Xinjiang, China

    Qi Zhang & Wei Zhang

  3. College of Resources and Environment, Tibet Agricultural and Animal Husbandry University, Linzhi, 860000, Tibet, China

    Huiying Xue & Zhiyong Ruan

  4. Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100050, China

    Yuqin Zhang

  5. Xinjiang Urumqi Maternal and Child Care Hospital, Ürümqi, 830001, Xinjiang, China

    Qi Zhang

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Correspondence to Wei Zhang or Zhiyong Ruan.

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The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain LAM7112T is MF784352. The GenBank accession number for the draft genome sequence of strain LAM7112T is RJAC00000000.

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Ma, Q., Zhang, Q., Jiang, X. et al. Actinoplanes solisilvae sp. nov., Isolated from Birch Forest Soil. Curr Microbiol 77, 3799–3806 (2020). https://doi.org/10.1007/s00284-020-02192-z

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  • DOI: https://doi.org/10.1007/s00284-020-02192-z

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