Abstract
To produce high-, medium- and low-molecular-weight hyaluronic acid (HA) at different temperatures using engineered Bacillus subtilis expressing hyaluronidase (HAase) from leech. By overexpressing the HAase gene hya in the HA-producing strain WmB using temperature-sensitive plasmid pKSV7, the engineered strain WmB-PYh produced HA with different molecular weights (8.61 kDa at 32 °C, 0.615 MDa at 42 °C, and 6.19 MDa at 47 °C). In this study, the molecular weight of HA was regulated by using leech HAase expressed from a temperature-sensitive plasmid. We thus obtained different molecular weight HAs by using a single bacterial strain at different culture temperatures.
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This work was supported by Tianjin Science Technology project, China (Grant No. 18YFZCSY00020, 16JCTPJC50100) and National Natural Science Foundation of China (31900050).
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Li, Y., Shi, Z., Shao, Y. et al. Temperature-controlled molecular weight of hyaluronic acid produced by engineered Bacillus subtilis. Biotechnol Lett 43, 271–277 (2021). https://doi.org/10.1007/s10529-020-03001-0
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DOI: https://doi.org/10.1007/s10529-020-03001-0