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Structural Features of Neuro-Glio-Vascular Ensembles in the Glomeruli of the Rat Olfactory Bulb

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Abstract

The glomeruli of the olfactory bulb of mammals are the primary coding elements of olfactory information. The excitation pattern produced by individual glomeruli in response to olfactory stimulation is stable and specific to certain odors. First of all, this is due to the structure of the neural circuits of the olfactory bulb. Nevertheless, there is reason to believe that auxiliary cells of the nervous system—astrocytes—play a role in the organization of the primary processing of the olfactory signal. It is known that astrocytes not only have a direct effect on synaptic activity and plasticity, but coordinate the joint work of neuronal circuits and the vascular component, forming so-called “neuro-glio-vascular ensembles” as well. In this study, we carried out a morphological study of peripheral processes and gap junctions of astrocytes in order to study the structure of neuro-glio-vascular ensembles at the level of organization of the olfactory bulb glomeruli neuropil. The study showed that the main part of the astrocytic processes inside the glomeruli is located in the area of dendro-dendritic connections of the interneurons and projection neurons of the olfactory bulb, while in the zone of the primary switching of the olfactory signal, the astrocytic processes are practically absent. We also found a pronounced imbalance in the expression of the main astroglial connexins between the different functional poles of the neuro-glio-vascular ensembles of the olfactory bulb and the presence of heterotypic contacts formed by Cx30. The functional significance of the observed features of connexins expression in the olfactory bulb has yet to be studied.

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Funding

This work was supported by a grant from the Russian Foundation for Basic Research, project no. 19-015-00325.

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Authors and Affiliations

  1. Laboratory of Functional Genomics and Morphology, Ivanovsky Academy of Biology and Biotechnology, Southern Federal University, 344090, Rostov-on-Don, Russia

    E. Yu. Kirichenko, A. K. Logvinov & S. Yu. Filippova

  2. Scientific and Research Technological Center for Neurotechnology, Southern Federal University, 344090, Rostov-on-Don, Russia

    R. A. Arefiev, V. G. Semynina & L.V. Lysenko

Authors
  1. E. Yu. Kirichenko
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  2. A. K. Logvinov
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  3. S. Yu. Filippova
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  4. R. A. Arefiev
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  5. V. G. Semynina
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  6. L.V. Lysenko
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Corresponding author

Correspondence to S. Yu. Filippova.

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Conflict of interests. The authors declare that they have no conflict of interest.

Statement on the welfare of animals. Animal keeping and experimental studies were carried out in accordance with the protocol approved by the Bioethics Commission of Southern Federal University on April 18, 2012.

Additional information

Abbreviations: OB—olfactory bulb, NGVE—euro-glio-vascular ensemble, GJ—gap junction; GFAP—glial fibrillar acidic protein.

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Kirichenko, E.Y., Logvinov, A.K., Filippova, S.Y. et al. Structural Features of Neuro-Glio-Vascular Ensembles in the Glomeruli of the Rat Olfactory Bulb. Cell Tiss. Biol. 14, 372–379 (2020). https://doi.org/10.1134/S1990519X20050053

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  • DOI: https://doi.org/10.1134/S1990519X20050053

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