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Applications and research advance of genome shuffling for industrial microbial strains improvement

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Abstract

Genome shuffling, an efficient and practical strain improvement technology via recursive protoplasts fusion, can break through the limits of species even genus to accelerate the directed evolution of microbial strains, without requiring the comprehensively cognized genetic background and operable genetic system. Hence this technology has been widely used for many important strains to obtain the desirable industrial phenotypes. In this review, we introduce the procedure of genome shuffling, discuss the new aid strategies of genome shuffling, summarize the applications of genome shuffling for increasing metabolite yield, improving strain tolerance, enhancing substrate utilization, and put forward the outlook to the future development of this technology.

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Acknowledgements

This work was supported by National Key R&D Program of China (2017YFC1600803), National natural science foundation project of China (31401543), Natural science foundation project of Henan province (182300410042), Innovative Research Team in University of Henan Province (19IRTSTHN008), Henan key laboratory of cereal resource transformation and utilization (PL2017005).

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Authors and Affiliations

  1. College of Bioengineering/Collaborative Innovation Center of Grain Storage Security in Henan Province, Henan University of Technology, Zhengzhou, 450001, People’s Republic of China

    Liang Chen, Qiu-Hua Xin, Li-Min Ma, Rui-Fang Li & Ke Bian

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  1. Liang Chen
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  2. Qiu-Hua Xin
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  3. Li-Min Ma
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Correspondence to Liang Chen or Ke Bian.

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Chen, L., Xin, QH., Ma, LM. et al. Applications and research advance of genome shuffling for industrial microbial strains improvement. World J Microbiol Biotechnol 36, 158 (2020). https://doi.org/10.1007/s11274-020-02936-w

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