Skip to main content
SpringerLink
Log in

Ultrastructure of Hepatocytes from Laboratory Mice Fed a Standard Dry Laboratory Animal Diet

  • Published:
Biochemistry (Moscow) Aims and scope Submit manuscript

Abstract

The significant destructive changes in ultrastructure of hepatocytes from laboratory mice kept in different vivariums in Moscow and fed with dry laboratory animal diets acquired from different domestic manufacturers that were not standardized for initial products were demonstrated using electron microscopy. Furthermore, disruption in the ultrastructure of liver parenchymal cells occurred regardless of the animal status (SPF or conventional), conditions of various vivariums, as well as the feed manufacturer. At the same time, studies on ultrastructure of liver hepatocytes from mice kept in the Charles River Laboratory facilities in Germany and fed with the Altromin Spezialfutter laboratory animal diet (GmbH & Co., Germany) that was produced using quality control of ingredients did not reveal destructive changes in the internal ultrastructure of hepatocytes. However, if these mice were later fed with the food produced in local manufactures, changes in the structure of liver cells developed after 2 months. Thus, feeding with dry diet from the domestic producers of an unspecified composition causes significant changes in the ultrastructure of hepatocytes in control animals, reflecting the development of some pathological processes in the body.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.
Fig. 3.
Fig. 4.
Fig. 5.
Fig. 6.
Fig. 7.
Fig. 8.
Fig. 9.

Similar content being viewed by others

Abbreviations

agEPR:

agranular EPR

EPR:

endoplasmic reticulum

grEPR:

granular EPR

IVC:

individually ventilated cage

NAFLD:

nonalcoholic fatty liver disease

SPF status:

specific-pathogen-free animals

VEC:

Complex of the Animal Facilities and the Research Unit Vivarium, Institute of Mitoengineering of Lomonosov Moscow State University

References

  1. Dalton, A. J., Kahler, H., Striebich, M. J., and Lloyd, B. (1950) Finer structure of hepatic, intestinal and renal cells of the mouse as revealed by the electron microscope, J. Natl. Cancer Inst., 11, 439-461.

    CAS  PubMed  Google Scholar 

  2. Fawcett, D. W. (1955) Observations on the cytology and electron microscopy of hepatic cells, J. Natl. Cancer Inst., 15, 1475-1503.

    CAS  PubMed  Google Scholar 

  3. Bruni, C., and Porter, K. R. (1965) The fine structure of the parenchymal cell of the normal rat liver: I. General observations, Am. J. Pathol., 46, 691-755.

    CAS  PubMed  PubMed Central  Google Scholar 

  4. Jones, A. L., and Fawcett, D. W. (1966) Hypertrophy of the agranular endoplasmic reticulum in hamster liver induced by phenobarbital (with a review on the functions of this organelle in liver), J. Histochem. Cytochem., 14, 215-232.

    Article  CAS  Google Scholar 

  5. Remmer, H., and Merker, H. J. (1965) Effect of drugs on the formation of smooth endoplasmic reticulum and drug-metabolizing enzymes, Ann. NY Acad. Sci., 123, 79-97.

    Article  CAS  Google Scholar 

  6. Steiner, J. W., Carruthers, J. S., and Kalifat, S. R. (1962) Observations on the fine structure of rat liver cells in extrahepatic cholestasis, Z. Zellforsch. Mikrosk. Anat., 58, 141-159.

    Article  CAS  Google Scholar 

  7. Wood, R. L. (1965) The fine structure of hepatic cells in chronic ethionine poisoning and during recovery, Am. J. Pathol., 46, 307-330.

    CAS  PubMed  PubMed Central  Google Scholar 

  8. Arhelger, R. B., Broom, J. S., and Boler, R. K. (1965) Ultrastructural hepatic alterations following tannic acid administration to rabbits, Am. J. Pathol., 46, 409-434.

    CAS  PubMed  PubMed Central  Google Scholar 

  9. Wieczorek, A., Stępień, P. M., Zarębska-Michaluk, D., Kryczka, W., Pabjan, P., and Król, T. (2017) Megamitochondria formation in hepatocytes of patient with chronic hepatitis C – a case report, Clin. Exp. Hepatol., 3, 169-175.

    Article  Google Scholar 

  10. Falcón, V., Acosta-Rivero, N., Chinea, G., Gavilondo, J., de la Rosa, M. C., Menéndez, I., Dueñas-Carrera, S., Viña, A., García, W., Gra, B., Noa, M., Reytor, E., Barceló, M. T., Alvarez, F., and Morales-Grillo, J. (2003) Ultrastructural evidences of HCV infection in hepatocytes of chronically HCV-infected patients, Biochem. Biophys. Res. Commun., 305, 1085-1090.

    Article  Google Scholar 

  11. Zhang, X. Q., Xu, C. F., Yu, C. H., Chen, W. X., and Li, Y. M. (2014) Role of endoplasmic reticulum stress in the pathogenesis of nonalcoholic fatty liver disease, World J. Gastroenterol., 20, 1768-1776.

    Article  CAS  Google Scholar 

  12. Cali, A. M., Zern, T. L., Taksali, S. E., de Oliveira, A. M., Dufour, S., Otvos, J. D., and Caprio, S. (2007) Intrahepatic fat accumulation and alterations in lipoprotein composition in obese adolescents: a perfect proatherogenic state, Diabetes Care, 30, 3093-3098.

    Article  CAS  Google Scholar 

  13. Fon Tacer, K., and Rozman, D. (2011) Nonalcoholic fatty liver disease: focus on lipoprotein and lipid deregulation, J. Lipids, 2011, 783976.

    Article  Google Scholar 

  14. Dallak, M. A., Bin-Jaliah, I., Albawardi, A., Haidara, M. A., Sakr, H. F., Eid, R. A., Hassan, W. N., and Al-Ani, B. (2018) Swim exercise training ameliorates hepatocyte ultrastructural alterations in rats fed on a high fat and sugar diet, Ultrastruct. Pathol., 42, 155-161.

    Article  Google Scholar 

  15. Abo El-Khair, S. M., Ghoneim, F. M., Shabaan, D. A., and Elsamanoudy, A. Z. (2020) Molecular and ultrastructure study of endoplasmic reticulum stress in hepatic steatosis: role of hepatocyte nuclear factor 4α and inflammatory mediators, Histochem. Cell Biol., 153, 49-62.

    Article  CAS  Google Scholar 

  16. Silva-Veiga, F. M., Rachid, T. L., de Oliveira, L., Graus-Nunes, F., Mandarim-de-Lacerda, C. A., and Souza-Mello, V. (2018) GW0742 (PPAR-beta agonist) attenuates hepatic endoplasmic reticulum stress by improving hepatic energy metabolism in high-fat diet fed mice, Mol. Cell Endocrinol., 474, 227-237.

    Article  CAS  Google Scholar 

  17. GOST R 50258-92 (1992) Combined Full-Rationed Feeds for Laboratory Animals, Technical Conditions, Standard Izd. (in Russian).

  18. Reynolds, E. S. (1963) The use of lead citrate at high pH as an electron-opaque stain in electron microscopy, J. Cell Biol., 17, 208-212.

    Article  CAS  Google Scholar 

  19. Silva, G. H., Hessel, G., Coelho, K. I., and Escanhoela, C. A. (2011) Steatosis of indeterminate cause in a pediatric group: is it a primary mitochondrial hepatopathy? Sao Paulo Med. J., 129, 217-223.

    Article  Google Scholar 

  20. Aragno, M., Tomasinelli, C. E., Vercellinatto, I., Catalano, M. G., Collino, M., Fantozzi, R., Danni, O., and Boccuzzi, G. (2009) SREBP-1c in nonalcoholic fatty liver disease induced by Western-type high-fat diet plus fructose in rats, Free Radic. Biol. Med., 47, 1067-1074.

    Article  CAS  Google Scholar 

  21. Meli, R., MattaceRaso, G., Irace, C., Simeoli, R., Di Pascale, A., Paciello, O., Pagano, T. B., Calignano, A., Colonna, A., and Santamaria, R. (2013) High fat diet induces liver steatosis and early dysregulation of iron metabolism in rats, PLoS One, 8, e66570.

    Article  CAS  Google Scholar 

  22. Pevzner, M. I. (1958) Principles of Dietotherapy (Acharkyan, A. I., and Marshak, M. S., eds.) Medgiz, Moscow (in Russian).

  23. Sihali-Beloui, O., Aroune, D., Benazouz, F., Hadji, A., El-Aoufi, S., and Marco, S. A. (2019) A hypercaloric diet induces hepatic oxidative stress, infiltration of lymphocytes, and mitochondrial reshuffle in Psammomys obesus, a murine model of insulin resistance, C R Biol., 342, 209-219, doi: https://doi.org/10.1016/j.crvi.2019.04.003 .

    Article  PubMed  Google Scholar 

Download references

Funding

This work was supported by the Russian Foundation for Basic Research (project no. 19-04-00578) and performed with support of TSITIS, Russia (project no. AAAA-A19-119012490166-2).

Author information

Authors and Affiliations

  1. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119991, Moscow, Russia

    V. B. Vays, I. M. Vangeli, O. A. Averina & L. E. Bakeeva

  2. Faculty of Biology, Lomonosov Moscow State University, 119234, Moscow, Russia

    M. L. Lovat

Authors
  1. V. B. Vays
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. I. M. Vangeli
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. O. A. Averina
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. L. Lovat
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. L. E. Bakeeva
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to L. E. Bakeeva.

Ethics declarations

The authors declare no conflicts of interests. All applicable international, national and/or institutional principles for the care and use of animals were followed.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Vays, V.B., Vangeli, I.M., Averina, O.A. et al. Ultrastructure of Hepatocytes from Laboratory Mice Fed a Standard Dry Laboratory Animal Diet. Biochemistry Moscow 85, 1082–1092 (2020). https://doi.org/10.1134/S0006297920090084

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S0006297920090084

Keywords

Search

Navigation