Skip to main content
SpringerLink
Log in

The Pattern of Glycogen Recovery in Muscles and Liver of Fish with Different Swimming Capabilities after being Caught in a Bottom Trawl

  • ORIGINAL PAPERS
  • Published:
Russian Journal of Marine Biology Aims and scope Submit manuscript

Abstract

The dynamics of glycogen recovery in the muscles and liver of the European flounder Plathichthys flesus (Linnaeus, 1758) and the European sprat Sprattus sprattus (Linnaeus, 1758) after exposure to trawl fishing has been studied. As the results show, the stress-inducing effect of fishing does not alter the glycogen content of the organs in the flounder, which leads a sedentary life, whereas a sharp decrease in glycogen is observed in the white and red muscles and the liver of the sprat, which is an actively swimming species. After capture, the dynamics of glycogen recovery in the organs of flounder has an undulatory pattern. The glycogen is completely restored within 12 h and is accompanied by a 100% survival rate of the flounder. The assumption is made that the resynthesis of glycogen in the organs of flounder occurs through the activation of carbohydrate metabolism in the liver. In sprat that had 3 h of “rest” after being caught and then placed in a 100‑liter tank to restore glycogen in tissues, a pronounced (4.5-fold) increase in the level of the polysaccharide was recorded from the white muscles, with a simultaneously low glycogen level in the liver. The glycogen compensation in the sprat possibly occurred through the reverse involvement of lactate in the polysaccharide resynthesis in the white muscles. The following additional 3-h period of rest was characterized by a significant (1.7-fold) decrease in the glycogen level in the white muscles compared to that in the previous rest period. No glycogen recovery in the sprat liver was observed throughout the entire rest period. Apparently, this organ is not involved in the polysaccharide recovery processes. The low survival rate of sprat (30%) is explained by the long exposure to stress caused by both capture and captivity in the tank, to which this “excitable” species is particularly sensitive.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1.
Fig. 2.

Similar content being viewed by others

REFERENCES

  1. Kukhta, V.K., Morozkina, T.E., Oletskii, Z.I., and Taganovich, A.D., Biologicheskaya khimiya: uchebnik (Biological Chemistry: A Textbook), Minsk: Asar, 2008.

  2. Minyuk, G.S., Shul’man, G.E., Shchepkin, V.Ya., and Yuneva, T.V., Chernomorskii shprot (svyaz’ dinamiki lipidov s biologiyei i promyslom) (Black Sea Sprat (The Relationship of Lipid Dynamics with Biology and Fisheries)), Sevastopol: EKOSI-Gidrofizika, 1997.

  3. Morozova, A.L., Astakhova, L.P., and Silkina, E.N., Carbohydrate metabolism in swimming fish, in Elementy fiziologii i biokhimii obshchego i aktivnogo obmena u ryb (Elements of Physiology and Biochemistry of General and Active Metabolism in Fish), Kiev: Naukova Dumka, 1978, ch. 7, pp. 122–143.

  4. Panin, L.E., Mokrushnikov, P.V., Knyazev, R.A., et al., Stress hormones and coronary syndrome X (experimental studies), Ateroskleroz, 2012, vol. 8, no. 2, pp. 5–13.

    Google Scholar 

  5. Plisetskaya, E.M., On the hormonal regulation of carbohydrate metabolism in lower vertebrates (cyclostomes and fishes), Extended Abstract of Doctoral (Biol.) Dissertation, Leningrad, 1972.

  6. Plisetskaya, E.M., Gormonal’naya regulyatsiya uglevodnogo obmena u nizshikh pozvonochnykh (Hormonal Regulation of Carbohydrate Metabolism in Lower Vertebrates), Leningrad: Nauka, 1975.

  7. Svetovidov, A.N., Ryby Chernogo morya (Fishes of the Black Sea), Moscow: Nauka, 1964.

  8. Silkina, E.N., Patterns of carbohydrate metabolism in skeletal muscles and liver of fish with different natural mobilities, Cand. Sci. (Biol.) Dissertation, Leningrad, 1991.

  9. Hochachka, P.W. and Somero, G.N., Strategies of Biochemical Adaptation, Philadelphia, Pa.: Saunders, 1973.

    Google Scholar 

  10. Shul’man, G.E., Fiziologo-biokhimicheskiye osobennosti godovykh tsiklov ryb (Physiological and Biochemical Characteristics of Annual Cycles in Fish), Moscow: Pishchevaya Promyshlennost’, 1972.

  11. Shul’man, G.E., Shchepkin, V.Ya., Yakovleva, K.K., and Khotkevich, T.V., Lipids and their use in swimming fish, in Elementy fiziologii i biokhimii obshchego i aktivnogo obmena u ryb (Elements of Physiology and Biochemistry of General and Active Metabolism in Fish), Kiev: Naukova Dumka, 1978, pp. 100–121.

  12. Emeretli, I.V. and Rusinova, O.S., Activity of enzymes of the main carbohydrate oxidation pathways in fish tissues, Gidrobiol. Zh., 2001, vol. 37, no. 1, pp. 79–81.

    CAS  Google Scholar 

  13. Black, E.C., Connor, A.R., Lam, K.-C., and Chiu, W.-G., Changes in glycogen, pyruvate and lactate in rainbow trout (Salmo gairdneri) during and following muscular activity, J. Fish. Res. Board Can., 1962, vol. 19, pp. 409–436.

    Article  CAS  Google Scholar 

  14. Frolow, J. and Milligan, C.L., Hormonal regulation of glycogen metabolism in white muscle slices from rainbow trout (Oncorhynchus mykiss Walbaum), Am. J. Physiol.: Regul., Integr. Comp. Physiol., 2004, vol. 287, pp. R1344–R1353.

    CAS  Google Scholar 

  15. Omlin, T. and Weber, J.-M., Exhausting exercise and tissue-specific expression of monocarboxylate transporters in rainbow trout, Am. J. Physiol.: Regul., Integr. Comp. Physiol., 2013, vol. 304, pp. R1036–R1043.

    CAS  Google Scholar 

  16. Pagnotta, A. and Milligan, C.L., The role of blood glucose in the restoration of muscle glycogen during recovery from exhaustive exercise in rainbow trout (Oncorhynchus mykiss) and winter flounder (Pseudopleuronectes americanus), J. Exp. Biol., 1991, vol. 161, pp. 489–508.

    CAS  PubMed  Google Scholar 

  17. Philp, A., Macdonald, A.L., and Watt, P.W., Lactate – a signal coordinating cell and systemic function, J. Exp. Biol., 2005, vol. 208, pp. 4561–4575.

    Article  CAS  Google Scholar 

  18. Seifter, S., Dayton, S., Novic, B., and Muntwyler, E., The estimation of glycogen with the anthrone reagent, Arch. Biochem. Biophys., 1950, vol. 25, no. 1, pp. 191–200.

    CAS  Google Scholar 

  19. West, T.G., Schulte, P.M., and Hochachka, P.W., Implications of hyperglycemia for post-exercise resynthesis of glycogen in trout skeletal muscle, J. Exp. Biol., 1994, vol. 189, pp. 69–84.

    CAS  PubMed  Google Scholar 

  20. Wang, Y., Wright, P.M., Heigenhauser, G.J., and Wood, C.M., Lactate transport by rainbow trout white muscle: kinetic characteristics and sensitivity to inhibitors, Am. J. Physiol.: Regul., Integr. Comp. Physiol., 1997, vol. 272, pp. R1577–R1587.

    CAS  Google Scholar 

  21. Weber, J.-M., Choi, K., Gonzalez, A., and Omlin, T., Metabolic fuel kinetics in fish: swimming, hypoxia and muscle membranes, J. Exp. Biol., 2016, vol. 219, pp. 250–258.

    Article  Google Scholar 

Download references

Funding

The study was conducted in the framework of the State Assignment, topic no. AAAA-A19-119012490045-0 Study of fundamental physical, physiologo-biochemical, reproductive, population, and behavioral characteristics of marine organisms.

Author information

Authors and Affiliations

  1. Vyazemsky Karadag Scientific Station–Nature Reserve, Russian Academy of Sciences, 298100, Feodosia, Russia

    E. N. Silkina, Yu. A. Silkin & M. Yu. Silkin

Authors
  1. E. N. Silkina
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yu. A. Silkin
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Yu. Silkin
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to E. N. Silkina.

Ethics declarations

Conflict of interests. The authors declare that they have no conflict of interest.

Statement on the welfare of animals. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

Additional information

Translated by E. Shvetsov

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Silkina, E.N., Silkin, Y.A. & Silkin, M.Y. The Pattern of Glycogen Recovery in Muscles and Liver of Fish with Different Swimming Capabilities after being Caught in a Bottom Trawl. Russ J Mar Biol 46, 270–276 (2020). https://doi.org/10.1134/S1063074020040070

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1063074020040070

Keywords:

Search

Navigation