Abstract
Intracellular signaling is a critical determinant of the normal growth and development of plants. Signaling peptides, also known as peptide hormones, along with classical phytohormones, are the significant players of plant intracellular signaling. C-terminally encoded peptide (CEP), a 15-amino acid post-translationally peptide identified in Arabidopsis, plays a pivotal role in lateral root formation, nodulation, and act as long-distance root to shoot signaling molecule in N-starvation conditions. Expression of CEP gene members in Arabidopsis is perturbed by nitrogen starvation; however, not much is known regarding their role in other abiotic stress conditions. To gain a comprehensive insight into CEP biology, we identified CEP genes across diverse plant genera (Glycine max, Sorghum bicolor, Brassica rapa, Zea mays, and Oryza sativa) using bioinformatics tools. In silico promoter analysis revealed that CEP gene promoters show an abundance of abiotic stress-responsive elements suggesting a possible role of CEPs in abiotic stress signaling. Spatial and temporal expression patterns of CEP via RNA seq and microarray revealed that various CEP genes are transcriptionally regulated in response to abiotic stresses. Validation of rice CEP genes expression by qRT-PCR showed that OsCEP1, OsCEP8, OsCEP9, and OsCEP10 were highly upregulated in response to different abiotic stress conditions. Our findings suggest these CEP genes might be important mediators of the abiotic stress response and warrant further overexpression/knockout studies to delineate their precise role in abiotic stress response.
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Abbreviations
- CEP:
-
C-terminally encoded peptide
- AA:
-
Amino acid
- CAREs:
-
Cis-acting regulatory elements
- qRT-PCR:
-
Quantitative real-time polymerase chain reaction
- ABA:
-
Abscisic acid
- ACC:
-
1-Aminocyclopropane 1-carboxylic acid
- JA:
-
Jasmonic acid
- SA:
-
Salicylic acid
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Funding was provided by Science and Engineering Research Board (Grant No. YSS/2015/00401) to SK.
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The idea, concept, design of experiments and manuscript preparation were done by AM. SK helped in developing the concept and critically reviewed the manuscript. SA, AS and KS carried out the bioinformatics analysis. Wet lab experiments were performed by SA. SK, JS and MJ helped in performing the experiments and preparation of manuscript.
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Aggarwal, S., Kumar, A., Jain, M. et al. C-terminally encoded peptides (CEPs) are potential mediators of abiotic stress response in plants. Physiol Mol Biol Plants 26, 2019–2033 (2020). https://doi.org/10.1007/s12298-020-00881-4
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DOI: https://doi.org/10.1007/s12298-020-00881-4