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Effect of composition and method of preparation of 2-hydroxyethyl methacrylate/gelatin hydrogels on biological in vitro (cell line) and in vivo (zebrafish) properties

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Abstract

We have studied the effect of compositions and methods of preparation on the physico-chemical and biocompatible behavior of the hydrogel matrices. Hydrogel matrices are synthesized by free radical polymerization of 2-hydroxyethyl methacrylate net and with gelatin. Highly porous hydrogel structures were obtained by porogenation, and by cryogenic treatment followed by freeze-drying. All samples were characterized for structural, morphological, absorption, degradation and in vitro (healthy human fibroblast cell line) and in vivo (zebrafish Danio rerio) biocompatible properties. The obtained results show that cryo samples, especially with gelatin show better, favorable absorption, morphological and biocompatible properties in comparison with PHEMA samples, which makes these materials highly attractive for biomedical uses.

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Acknowledgments

This work was financed by the SCOPES program of the Swiss National Science Foundation (SNSF) and the Swiss Agency for Development and Cooperation (SDC) (Grants No IZ73ZO_152327). This work has been supported by the Ministry for Education, Science and Technological Development of the Republic of Serbia (Contracts No. 451-03-68/2020-14/172062 and 451-03-68/2020-14/172026).

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Authors and Affiliations

  1. Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, Belgrade, 11 000, Serbia

    Simonida Lj. Tomić, Marija M. Babić & Jovana S. Vuković

  2. Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, Belgrade, Serbia

    Lidija Djokić, Aleksandar Pavić & Jasmina Nikodinovic-Runić

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  1. Simonida Lj. Tomić
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  2. Marija M. Babić
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  3. Jovana S. Vuković
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  4. Lidija Djokić
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  6. Jasmina Nikodinovic-Runić
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Correspondence to Simonida Lj. Tomić.

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Tomić, S.L., Babić, M.M., Vuković, J.S. et al. Effect of composition and method of preparation of 2-hydroxyethyl methacrylate/gelatin hydrogels on biological in vitro (cell line) and in vivo (zebrafish) properties. J Polym Res 27, 305 (2020). https://doi.org/10.1007/s10965-020-02219-w

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