Abstract
This special review is written to commemorate the life and contributions of Dr. Trevor Alleyne Thorpe, who passed away on May 18, 2020, at the age of 83 yr (https://www.sivb.org/InVitroReport/issue-54-2-april-june-2020/in-memoriam-4/). As part of his academic legacy, Trevor had trained an impressive number of laboratory members—15 PhD students, 14 Master’s students, 25 postdoctoral fellows, and many visiting scientists—who collectively identify themselves as proud members of “Team Thorpe.” This executive summary version of his major scientific contributions was prepared by the team.
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References
Aitken J, Horgan KJ, Thorpe TA (1981) Influence of explant selection on the shoot-forming capacity of juvenile tissue of Pinus radiata. Can J For Res 11:112–117
Ashihara H, Stasolla C, Loukanina N, Thorpe TA (2001) Purine metabolism during white spruce somatic embryo development: salvage of adenine, adenosine, and inosine. Plant Sci 160:647–657
Ashihara H, Stasolla C, Yin Y, Loukanina N, Thorpe TA (2005) De novo and salvage biosynthetic pathways of pyridine nucleotides and nicotinic acid conjugates in cultured plant cells. Plant Sci 169:107–114
Beaudoin-Eagan LD, Thorpe TA (1985) Tyrosine and phenylalanine ammonia lyase activities during shoot initiation in tobacco callus cultures. Plant Physiol 78:438–441
Bender L, Joy IV, Thorpe TA (1987) Studies on [14C]-glucose metabolism during shoot bud induction in cultured cotyledon explants of Pinus radiata. Physiol Plant 69:428–434
Brown DCW, Leung DWM, Thorpe TA (1979) Osmotic requirement for shoot formation in tobacco callus. Physiol Plant 46:36–41
Chandler SF, Paek KY, Hachey JE, Thorpe TA (1989) Sodium sulphate tolerance in Beta vulgaris (sugar beet) callus cultures. Bot Gaz 150:247–250
Chandler SF, Ragolsky E, Thorpe TA (1988) Sodium sulphate tolerance and sensitivity in tissue cultures and regenerated plants of Nicotiana tabacum. J Plant Physiol 132:155–159
Chandler SF, Thorpe TA (1986) Variation from plant tissue cultures: biotechnological application to improving salinity tolerance. Biotechnol Adv 4:117–135
Chandler SF, Thorpe TA (1987) Characterization of growth, water relations and proline accumulation in sodium sulphate tolerant callus of Brassica napus L. cv. Westar. Plant Physiol 84:106–111
Dhindsa RS, Plumb-Dhindsa P, Thorpe TA (1981) Leaf senescence: correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase. J Exp Bot 32:93–101
Douglas TJ, Villalobos VM, Thompson MR, Thorpe TA (1982) Lipid and pigment changes during shoot initiation in cultured explants of Pinus radiata. Physiol Plant 55:470–477
Gamborg OL, Murashige T, Thorpe TA, Vasil IK (1976) Plant tissue culture media. In Vitro Cell Dev Biol - Plant 12:473–478
Harry IS, Lu C, Sharma KK, Thorpe TA (1994) Micropropagation of western hemlock (Tsuga heterophylla [Raf.] Sarg.) from embryonic explants. New Forest 8:1–13
Harry IS, Pulido CM, Thorpe TA (1995) Plantlet regeneration from mature embryos of Juniperus cedrus. Tree Physiol 15:727–738
Harry IS, Thompson MR, Lu C, Thorpe TA (1987) In vitro plantlet formation from embryonic explants of eastern white cedar (Thuja occidentalis L.). Tree Physiol 3:273–283
Harry IS, Thompson MR, Thorpe TA (1991) Regeneration of plantlets from mature embryos of western larch. In Vitro Cell Dev Biol - Plant 27:89–98
Harry IS, Thorpe TA (1991) Somatic embryogenesis and plant regeneration from mature zygotic embryos of red spruce. Bot Gaz 152:446–452
Harry IS, Thorpe TA (1994) Regeneration of plantlets through organogenesis from mature embryos of jack pine. Plant Cell Tissue Organ Cult 37:159–164
Huxter TJ, Thorpe TA, Reid DM (1981) Shoot initiation in light- and dark-grown tobacco callus: the role of ethylene. Physiol Plant 53:319–326
Joy RW IV, Vogel HJ, Thorpe TA (1997) Inorganic nitrogen metabolism in embryogenic white spruce cultures: a nitrogen 14/15 NMR study. J Plant Physiol 151:306–315
Kim HR, Patel KR, Thorpe TA (1985) Regeneration of mulberry plantlets through tissue culture. Bot Gaz 146:335–340
Krishnaraj S, Thorpe TA (1996) Salinity stress effects on [14C-1]- and [14C-6]-glucose metabolism of a salt-tolerant and salt-susceptible variety of wheat. Int J Plant Sci 157:110–117
Kulchetscki L, Harry IS, Yeung EC, Thorpe TA (1995) In vitro regeneration of Pacific silver fir (Abies amabilis) plantlets and histological analysis of shoot formation. Tree Physiol 15:727–738
Kumar PP, Bender L, Thorpe TA (1988) Activities of ribulose bisphosphate carboxylase and phosphoenolpyruvate carboxylase and 14C-bicarbonate fixation during in vitro culture of Pinus radiata cotyledons. Plant Physiol 87:675–679
Kumar PP, Lakshmanan P, Thorpe TA (1998) Regulation of morphogenesis in plant tissue culture by ethylene. In Vitro Cell Dev Biol - Plant 34:94–103
Kumar PP, Reid DM, Thorpe TA (1987) The role of ethylene and carbon dioxide in differentiation of shoot buds in excised cotyledons of Pinus radiata in vitro. Physiol Plant 69:244–252
Lambardi M, Harry IS, Menabeni D, Thorpe TA (1995) Organogenesis and somatic embryogenesis in Cupressus sempervirens. Plant Cell Tissue Organ Cult 40:179–182
Lu C, Harry IS, Thompson MR, Thorpe TA (1991) Plantlet regeneration from cultured embryos and seedling parts of red spruce (Picea rubens Sarg.). Bot Gaz 152:42–50
Lu C, Thorpe TA (1987) Somatic embryogenesis and plantlet regeneration in cultured immature embryos of Picea glauca. J Plant Physiol 128:297–302
Muriithi WT, Harry IS, Yeung EC, Thorpe TA (1993) Plantlet regeneration in chir pine (Pinus roxburghii Sarg.): morphogenesis and histology. Forest Ecol Management 57:141–160
Nour KA, Thorpe TA (1994) The effect of the gaseous state on bud induction and shoot multiplication in vitro in eastern white cedar. Physiol Plant 90:163–172
Paek K, Chandler SF, Thorpe TA (1987) Micropropagation of Raphanus sativus var. longipinnatus (Japanese radish) cultivar Gungjung. Plant Cell Tissue Organ Cult 9:159–166
Paek KY, Chandler SF, Thorpe TA (1988) Physiological effects of sodium sulphate and sodium chloride on callus cultures of Brassica campestris (Chinese cabbage). Physiol Plant 72:160–166
Patel KR, Shekhawat NS, Berlyn GP, Thorpe TA (1984) Isolation and culture of protoplasts from cotyledons of Pinus coulteri D. Don. Plant Cell Tissue Organ Cult 3:85–90
Patel KR, Thorpe TA (1984) Histochemical examination of shoot initiation in cultured cotyledon explants of radiata pine. Bot Gaz 145:312–322
Pua EC, Ragolsky E, Thorpe TA (1985) Retention of shoot regeneration capacity of tobacco callus by Na2SO4. Plant Cell Rep 4:225–228
Pua EC, Thorpe TA (1986) Differential response of nonselected and Na2SO4-selected callus cultures of Beta vulgaris L. to salt stress. J Plant Physiol 123:241–248
Pulido CM, Harry IS, Thorpe TA (1994) Effect of various induction treatments on elongation and rooting of adventitious of Canary Island pine (Pinus canariensis). Plant Cell Tissue Organ Cult 39:225–230
Rahman MH, Krishnaraj S, Thorpe TA (1995) Selection for salt tolerance in vitro using microspore-derived embryos of Brassica napus cv. topas, and the characterization of putative tolerant plants. In Vitro Cell Dev Biol - Plant 31:116–121
Rumary C, Patel KR, Thorpe TA (1986) Plantlet formation in black and white spruce. II. Histological analysis of adventitious shoot formation in vitro. Can J Bot 64:997–1002
Stasolla C, Loukanina N, Ashihara H, Yeung EC, Thorpe TA (2001a) Purine and pyrimidine metabolism during the partial drying treatment of white spruce (Picea glauca ) somatic embryos. Physiol Plant 111:93–101
Stasolla C, Loukanina N, Ashihara H, Yeung EC, Thorpe TA (2001b) Ascorbic acid changes the pattern of purine metabolism during germination of white spruce somatic embryos. Tree Physiol 21:359–367
Thompson MR, Thorpe TA (1997) Analysis of protein patterns during shoot initiation in cultured Pinus radiata cotyledons. J Plant Physiol 151:724–734
Thorpe TA, Harry IS, Kumar PP (1991) Application of micropropagation to forestry. In: Debergh PC, Zimmerman RH (eds) Micropropagation. Springer, Dordrecht, pp 311–336. https://doi.org/10.1007/978-94-009-2075-0_21
Thorpe TA, Murashige T (1968) Starch accumulation in shoot-forming tobacco callus cultures. Science 160:421–422
Thorpe TA, Murashige T (1970) Some histochemical changes underlying shoot initiation in tobacco callus cultures. Can J Bot 48:277–285
Thorpe TA, Yeung EC (eds) (2011) Plant embryo culture: methods and protocols. Humana Press, Totowa. https://doi.org/10.1007/978-1-61737-988-8
Villalobos VM, Oliver MJ, Yeung EC, Thorpe TA (1984) Cytokinin-induced switch in development in excised cotyledons of radiata pine cultured in vitro. Physiol Plant 61:483–489
Yeung EC, Aitken J, Biondi S, Thorpe TA (1981) Shoot histogenesis in cotyledon explants of radiata pine. Bot Gaz 142:494–501
Acknowledgments
We thank Dr. Pratibha Ravindran for helping to prepare the illustrations and all the members of Team Thorpe who shared their views on this review.
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Editor: Gregory Phillips
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Kumar, P.P., Chandler, S.F., Harry, I.S. et al. Trevor Alleyne Thorpe: His academic life and scientific legacy. In Vitro Cell.Dev.Biol.-Plant 56, 728–737 (2020). https://doi.org/10.1007/s11627-020-10118-4
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DOI: https://doi.org/10.1007/s11627-020-10118-4