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Mash1-expressing cells may be relevant to type III cells and a subset of PLCβ2-positive cell differentiation in adult mouse taste buds

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Abstract

Mammalian taste bud cells have a limited lifespan and differentiate into type I, II, and III cells from basal cells (type IV cells) (postmitotic precursor cells). However, little is known regarding the cell lineage within taste buds. In this study, we investigated the cell fate of Mash1-positive precursor cells utilizing the Cre-loxP system to explore the differentiation of taste bud cells. We found that Mash1-expressing cells in Ascl1CreERT2::CAG-floxed tdTomato mice differentiated into taste bud cells that expressed aromatic L-amino acid decarboxylase (AADC) and carbonic anhydrase IV (CA4) (type III cell markers), but did not differentiate into most of gustducin (type II cell marker)-positive cells. Additionally, we found that Mash1-expressing cells could differentiate into phospholipase C β2 (PLCβ2)-positive cells, which have a shorter lifespan compared with AADC- and CA4-positive cells. These results suggest that Mash1-positive precursor cells could differentiate into type III cells, but not into most of type II cells, in the taste buds.

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Acknowledgments

The authors would like to thank Enago (www.enago.jp) for the English language review.

Funding

The work was supported by the Japan Society for the Promotion Grant-in-Aid for Scientific Research number 18 K09525 to Y.S.

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Authors and Affiliations

  1. Division of Orofacial Functions and Orthodontics, Kyushu Dental University, 2-6-1 Manazuru, Kokurakita-ku, Kitakyushu, 803-8580, Japan

    Chia-Chien Hsu, Kaori K. Gunjigake, Kayoko N. Kuroishi & Tatsuo Kawamoto

  2. Division of Anatomy, Department of Health Improvement, Kyushu Dental University, 2-6-1 Manazuru, Kokurakita-ku, Kitakyushu, 803-8580, Japan

    Chia-Chien Hsu, Yuji Seta, Kae Matsuyama, Shinji Kataoka, Mitsushiro Nakatomi & Takashi Toyono

Authors
  1. Chia-Chien Hsu
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  2. Yuji Seta
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  3. Kae Matsuyama
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  4. Shinji Kataoka
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  5. Mitsushiro Nakatomi
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  6. Takashi Toyono
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  7. Kaori K. Gunjigake
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  8. Kayoko N. Kuroishi
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  9. Tatsuo Kawamoto
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Correspondence to Yuji Seta.

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The authors state that there is no conflict of interest.

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All of the animals used in this study were maintained and handled according to the protocols approved by Kyushu Dental University Animal Care (Certification No: 17–010).

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Hsu, CC., Seta, Y., Matsuyama, K. et al. Mash1-expressing cells may be relevant to type III cells and a subset of PLCβ2-positive cell differentiation in adult mouse taste buds. Cell Tissue Res 383, 667–675 (2021). https://doi.org/10.1007/s00441-020-03283-w

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  • DOI: https://doi.org/10.1007/s00441-020-03283-w

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