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Characterization and functional analysis of Krtap11-1 during hair follicle development in Angora rabbits (Oryctolagus cuniculus)

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Abstract

Background

Keratin-associated protein (KAP), the structural protein molecule of hair fibers, plays a key role in determining the physical properties of hair. Studies of Krtap11-1 have focused only on its localization. Functional studies of Krtap11-1 in hair follicle development have so far not been reported.

Objective

This study aimed to provide evidence for the role of Krtap11-1 in skin and hair development.

Methods

Full-length cloning and analysis of Krtap11-1 were conducted to ascertain its function. Overexpression vectors and interference sequences were constructed and transfected into RAB-9 cells. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to investigate the hair follicle developmental stage of Krtap11-1, the expression of different tissues, and the effects on other hair follicle development-related genes.

Results

The full length of cloned Krtap11-1 was 947 bp. Krtap11-1 was confirmed to be a hydrophilic protein localized mostly in mitochondria. The greatest mRNA expression was observed in skin. Using a follicle synchronization model, it was found that Krtap11-1 mRNA expression levels first increased then decreased over the passage of time, principally during hair follicle catagen and telogen. Following the overexpression of Krtap11-1, mRNA expression levels of the WNT-2, KRT17, BMP-2, and TGF-β-1 genes increased, and LEF-1 decreased (P < 0.05), the converse after the corresponding use of si-RNA interference.

Conclusions

Krtap11-1 exerts a promoting effect. The results provide novel insight into the relationship between hair follicle development and Krtap11-1 gene expression.

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Acknowledgements

This work was funded by Modern Agricultural Industrial System Special Funding (CARS-43-A-1) and the national key research and development program of China (2018YFD0502203). Thanks to reviewers and editors for their helpful comments on this article.

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Authors and Affiliations

Authors

Contributions

SL was responsible for the collection and analysis of results and wrote the manuscript. BZ, YC, SH, NY and JL are responsible for sample collection and experimental guidance. TZ and ZB carried out of the experiments. SL and XW designed the study and finalized the manuscript. All authors read and approved.

Corresponding author

Correspondence to Xinsheng Wu.

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Conflicts of interest

The authors declare that they have no conflicts of interest.

Human and animal rights

All animal procedures were approved by the Animal Care and Use Committee of Yangzhou University, and performed in accordance with the Regulations for the Administration of Affairs Concerning Experimental Animals (China, 1988).

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Liang, S., Bao, Z., Zhao, B. et al. Characterization and functional analysis of Krtap11-1 during hair follicle development in Angora rabbits (Oryctolagus cuniculus). Genes Genom 42, 1281–1290 (2020). https://doi.org/10.1007/s13258-020-00995-9

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  • DOI: https://doi.org/10.1007/s13258-020-00995-9

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