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Evaluation of Both SPAD Reading and SPAD Index on Estimating the Plant Nitrogen Status of Winter Wheat

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Abstract

Rapid non-destructive estimation of the plant nitrogen (N) status is needed for the precise management of N in small-scale farms. Our objectives were to describe the responses of the soil–plant analyses development (SPAD) measurements to the plant N status of winter wheat, and to evaluate whether the SPAD index is more precise than SPAD readings in estimating the plant N status. During 2009–2011, a field experiment with winter wheat was conducted in the North China Plain. There were eight N supply levels ranging from 0 to 420 kg N ha−1 to create gradients of leaf greenness. The SPAD readings were measured on the topmost fully expanded leaf; the SPAD index was expressed relative to the SPAD readings of sufficiently fertilized plants. Combing over years, significant quadratic responses of SPAD reading to the plant N concentration (PNC) occurred at each/similar Zadoks growth stage (ZGS) but differed largely from ZGS 39/43 to ZGS 58/59, the r2 varied between 0.88 and 0.97; the SPAD readings exhibited a high logarithmic correlation to plant N accumulation (PNA, r2 = 0.94) irrespective of growth stages. Compared with SPAD readings, the SPAD index showed the same precision in response to PNC and PNA at each growth stage in each year, but presented less precision for the combined datasets across years, indicating that the SPAD index is not a more preferable tool in estimating plant N status. In addition, both the SPAD readings and SPAD index demonstrated poor to moderate responses to basal stem nitrate content (BSNC) with large differences among ZGS 39–59 and between the 2 years, indicating that the SPAD measurements could not be effectively used to estimate the BSNC.

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References

  • Ahmed, S. U. (2011). Effects of soil water deficit on leaf nitrogen, chlorophylls and SPAD chlorphyll meter reading on growth stages of soybean. Bangladesh Journal of Botany,40(2), 171–175.

    Article  Google Scholar 

  • Arunyanark, A., Jogloy, S., Akkasaeng, C., Vorasoot, N., Kesmala, T., Nageswara Rao, R. C., et al. (2008). Chlorophyll stability is an indicator of drought tolerance in peanut. Journal of Agronomy and Crop Science,194(2), 113–125. https://doi.org/10.1111/j.1439-037X.2008.00299.x.

    Article  CAS  Google Scholar 

  • Barraclough, P. B., & Kyte, J. (2001). Effect of water stress on chlorophyll meter readings in winter wheat. In W. J. Horst, M. K. Schenk, A. Bürkert, N. Claassen, H. Flessa, W. B. Frommer, et al. (Eds.), Plant nutrition: food security and sustainability of agro-ecosystems through basic and applied research (pp. 722–723). Dordrecht: Springer.

    Chapter  Google Scholar 

  • Barutcular, C., Yıldırım, M., Koç, M., Akıncı, C., Toptaş, I., Albayrak, O., et al. (2016). Evaluation of SPAD chlorophyll in spring wheat genotypes under different environments. Fresenius Environmental Bulletin,25(4), 1258–1266.

    CAS  Google Scholar 

  • Byju, G., & Anand, M. H. (2009). Leaf color chart and chlorophyll-meter-based leaf nitrogen estimation and their threshold values for real-time nitrogen management in Cassava. Communications in Soil Science and Plant Analysis,40(17–18), 2816–2832. https://doi.org/10.1080/00103620903173830.

    Article  CAS  Google Scholar 

  • Chen, P., Haboudane, D., Tremblay, N., Wang, J., Vigneault, P., & Li, B. (2010). New spectral indicator assessing the efficiency of crop nitrogen treatment in corn and wheat. Remote Sensing of Environment,114(9), 1987–1997. https://doi.org/10.1016/j.rse.2010.04.006.

    Article  Google Scholar 

  • Debaeke, P., Rouet, P., & Justes, E. (2006). Relationship between the normalized SPAD index and the nitrogen nutrition index: application to durum wheat. Journal of Plant Nutrition,29(1), 75–92. https://doi.org/10.1080/01904160500416471.

    Article  CAS  Google Scholar 

  • Dunn, B. L., Singh, H., & Goad, C. (2018). Relationship between chlorophyll meter readings and nitrogen in poinsettia leaves. Journal of Plant Nutrition,41(12), 1–10.

    Article  Google Scholar 

  • Feng, W., Zhu, Y., Yao, X., Tian, Y., Zhuang, S., & Cao, W. (2008). Monitoring plant nitrogen accumulation dynamics with hyperspectral remote sensing in wheat. Scientia Agricultura Sinica,41(7), 1937–1946.

    CAS  Google Scholar 

  • Hawkins, J. A., Sawyer, J. E., Barker, D. W., & Lundvall, J. P. (2007). Using relative chlorophyll meter values to determine nitrogen application rates for corn. Agronomy Journal,99(4), 1034–1040. https://doi.org/10.2134/agronj2006.0309.

    Article  CAS  Google Scholar 

  • Horneck, D. A., & Miller, R. O. (1998). Determination of total nitrogen in plant tissue. Handbook of reference methods for plant analysis,2, 75–83.

    Google Scholar 

  • Jarrell, W., & Beverly, R. (1981). The dilution effect in plant nutrition studies. Advances in Agronomy,34(1), 197–224.

    Article  CAS  Google Scholar 

  • Jia, L., Yu, Z., Li, F., Gnyp, M., Koppe, W., Bareth, G., et al. (2012). Nitrogen status estimation of winter wheat by using an IKONOS satellite image in the North China Plain. In D. Li & Y. Chen (Eds.), Computer and computing technologies in agriculture V (pp. 174–184). Berlin: Springer.

    Chapter  Google Scholar 

  • Kasahara, M., Kagawa, T., Oikawa, K., Suetsugu, N., Miyao, M., & Wada, M. (2002). Chloroplast avoidance movement reduces photodamage in plants. Nature,420(6917), 829–832.

    Article  CAS  Google Scholar 

  • Li, F., Gnyp, M. L., Jia, L., Miao, Y., Yu, Z., Koppe, W., et al. (2008). Estimating N status of winter wheat using a handheld spectrometer in the North China Plain. Field Crops Research,106(1), 77–85. https://doi.org/10.1016/j.fcr.2007.11.001.

    Article  Google Scholar 

  • Li, Z., Liu, H., & Zhang, F. (2003). Research of nitrogen nutrition status for winter wheat based on chlorophyll meter. Plant Nutrition and Fertilizer Science,9(4), 401–405.

    Google Scholar 

  • Li, D., Wang, X., Zheng, H., Zhou, K., Yao, X., Tian, Y., et al. (2018). Estimation of area- and mass-based leaf nitrogen contents of wheat and rice crops from water-removed spectra using continuous wavelet analysis. Plant Methods,14(1), 76.

    Article  Google Scholar 

  • Li, Y. X., Xu, D. F., Xie, X. J., Shen, S. H., & Pan, W. B. (2009). Spatial distribution of winter wheat leaf SPAD value and its relationship with nitrogen nutrition status. Chinese Journal of Agrometeorology,30(2), 164–168.

    CAS  Google Scholar 

  • Li, J. W., Yang, J. P., Li, D. S., Fei, P. P., Guo, T. T., Ge, C. S., et al. (2011). Chlorophyll meter’s estimate of weight-based nitrogen concentration in rice leaf is influenced by leaf thickness. Plant Production Science,14(2), 177–183.

    Article  Google Scholar 

  • Lopez-Bellido, R. J., Shepherd, C. E., & Barraclough, P. B. (2004). Predicting post-anthesis N requirements of bread wheat with a Minolta SPAD meter. European Journal of Agronomy,20(3), 313–320. https://doi.org/10.1016/s1161-0301(03)00025-x.

    Article  Google Scholar 

  • Marenco, R. A., Antezana-Vera, S. A., & Nascimento, H. C. S. (2009). Relationship between specific leaf area, leaf thickness, leaf water content and SPAD-502 readings in six Amazonian tree species. Photosynthetica,47(2), 184–190. https://doi.org/10.1007/s11099-009-0031-6.

    Article  Google Scholar 

  • Monostori, I., Árendás, T., Hoffman, B., Galiba, G., Gierczik, K., Szira, F., et al. (2016). Relationship between SPAD value and grain yield can be affected by cultivar, environment and soil nitrogen content in wheat. Euphytica,211(1), 103–112. https://doi.org/10.1007/s10681-016-1741-z.

    Article  CAS  Google Scholar 

  • Munoz-Huerta, R. F., Guevara-Gonzalez, R. G., Contreras-Medina, L. M., Torres-Pacheco, I., Prado-Olivarez, J., & Ocampo-Velazquez, R. V. (2013). A review of methods for sensing the nitrogen status in plants: advantages, disadvantages and recent advances. Sensors (Basel),13(8), 10823–10843. https://doi.org/10.3390/s130810823.

    Article  CAS  Google Scholar 

  • Oppelt, N. (2002). Monitoring of plant chlorophyll and nitrogen status using the airborne imaging spectrometer AVIS. Dissertation, LMU München.

  • Peltonen, J., Virtanen, A., & Haggrèn, E. (1995). Using a chlorophyll meter to optimize nitrogen fertilizer application for intensively-managed small-grain cereals. Journal of Agronomy and Crop Science,174(5), 309–318. https://doi.org/10.1111/j.1439-037X.1995.tb01118.x.

    Article  CAS  Google Scholar 

  • Prost, L., & Jeuffroy, M. H. (2007). Replacing the nitrogen nutrition index by the chlorophyll meter to assess wheat N status. Agronomy for Sustainable Development,27(4), 321–330. https://doi.org/10.1051/agro:2007032.

    Article  Google Scholar 

  • Ravier, C., Quemada, M., & Jeuffroy, M.-H. (2017). Use of a chlorophyll meter to assess nitrogen nutrition index during the growth cycle in winter wheat. Field Crops Research,214, 73–82. https://doi.org/10.1016/j.fcr.2017.08.023.

    Article  Google Scholar 

  • Schächtl, J., Huber, G., Maidl, F.-X., Sticksel, E., Schulz, J., & Haschberger, P. (2005). Laser-induced chlorophyll fluorescence measurements for detecting the nitrogen status of wheat (Triticum aestivum L.) canopies. Precision Agriculture,6(2), 143–156. https://doi.org/10.1007/s11119-004-1031-y.

    Article  Google Scholar 

  • Schlemmer, M., Gitelson, A., Schepers, J., Ferguson, R., Peng, Y., Shanahan, J., et al. (2013). Remote estimation of nitrogen and chlorophyll contents in maize at leaf and canopy levels. International Journal of Applied Earth Observation and Geoinformation,25, 47–54. https://doi.org/10.1016/j.jag.2013.04.003.

    Article  Google Scholar 

  • Spaner, D., Todd, A. G., Navabi, A., McKenzie, D. B., & Goonewardene, L. A. (2005). Can leaf chlorophyll measures at differing growth stages be used as an indicator of winter wheat and spring barley nitrogen requirements in eastern Canada? Journal of Agronomy and Crop Science,191(5), 393–399. https://doi.org/10.1111/j.1439-037X.2005.00175.x.

    Article  Google Scholar 

  • Tian, Y. C., Zhu, Y., Cao, W. X., Fan, X. M., & Liu, X. J. (2003). Monitoring protein and starch accumulation in wheat grains with leaf SPAD and canopy spectral reflectance. Agricultural Sciences in China,2(11), 1205–1211.

    Google Scholar 

  • Uchino, H., Watanabe, T., Ramu, K., Sahrawat, K. L., Marimuthu, S., Wani, S. P., et al. (2013). Calibrating chlorophyll meter (SPAD-502) reading by specific leaf area for estimating leaf nitrogen concentration in sweet sorghum. Journal of Plant Nutrition,36(10), 1640–1646. https://doi.org/10.1080/01904167.2013.799190.

    Article  CAS  Google Scholar 

  • Varvel, G. E., Schepers, J. S., & Francis, D. D. (1997). Ability for in-season correction of nitrogen deficiency in corn using chlorophyll meters. Soil Science Society of America Journal,61(4), 1233–1239.

    Article  CAS  Google Scholar 

  • Yu, W., Miao, Y., Feng, G., Yue, S., & Liu, B. (2012). Evaluating different methods of using chlorophyll meter for diagnosing nitrogen status of summer maize. In 2012 First International Conference on Agro-Geoinformatics, (pp. 1–4): IEEE.

  • Yue, X. L., Hu, Y. C., Zhang, H. Z., & Schmidhalter, U. (2015). Green Window approach for improving nitrogen management by farmers in small-scale wheat fields. The Journal of Agricultural Science,153(03), 446–454. https://doi.org/10.1017/s0021859614000203.

    Article  Google Scholar 

  • Yue, X., Hu, Y., Zhang, H., & Schmidhalter, U. (2019). Optimizing the nitrogen management strategy for winter wheat in the North China Plain using rapid soil and plant nitrogen measurements. Communications in Soil Science and Plant Analysis,50(11), 1310–1320. https://doi.org/10.1080/00103624.2019.1604738.

    Article  CAS  Google Scholar 

  • Zadoks, J. C., Chang, T. T., & Konzak, C. F. (1974). A decimal code for the growth stages of cereals. Weed Research,14(6), 415–421.

    Article  Google Scholar 

  • Zhang, Y., Chen, X. M., Shao, F. C., Wang, D. S., & He, Z. H. (2012). Analysis on quality stability of steamed bread and noodle by wheat cultivar Zhongmai 175. Journal of Triticeae Crops,32(3), 440–447.

    Google Scholar 

  • Zhang, F. S., Wang, J. Q., Zhang, W. F., Cui, Z. L., Ma, W. Q., Chen, X. P., et al. (2008). Nutrient use efficiencies of major cereal crops in China and measures for improvement. Acta Pedologica Sinica,45(5), 915–924.

    Google Scholar 

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Acknowledgements

This work was supported by the German Federal Ministry of Education and Research (BMBF, Project Number: FKZ 0330800A).

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Authors and Affiliations

  1. National Engineering Laboratory for Improving Fertility of Arable Soils, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Zhongguancun South Road 12, Haidian District, Beijing, 100081, People’s Republic of China

    Xianlu Yue & Huaizhi Zhang

  2. Department of Plant Sciences, Chair of Plant Nutrition, Technische Universität München, Emil-Ramann-Str. 2, 85354, Freising, Germany

    Xianlu Yue, Yuncai Hu & Urs Schmidhalter

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  1. Xianlu Yue
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Correspondence to Xianlu Yue or Urs Schmidhalter.

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Yue, X., Hu, Y., Zhang, H. et al. Evaluation of Both SPAD Reading and SPAD Index on Estimating the Plant Nitrogen Status of Winter Wheat. Int. J. Plant Prod. 14, 67–75 (2020). https://doi.org/10.1007/s42106-019-00068-2

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