Osteogenic differentiation of human bone marrow-derived mesenchymal stem cells is enhanced by an aragonite scaffold
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Graphical abstract
Keywords
Aragonite-based implant
Coralline scaffold
Osteogenesis
Bone marrow-derived mesenchymal stem cell
Energy dispersive x-ray spectroscopy
Agili-C
Abbreviations
ALP
alkaline phosphatase
AR
Alizarin red
BGLAP
bone gamma-carboxyglutamate
BM-MSC
bone marrow-derived mesenchymal stem cell
CC
control culture
DCP
dicalcium phosphate anhydrous
ECM
extracellular matrix
EDS
energy dispersive X-ray spectroscopy
GAPDH
glyceraldehyde 3-phosphate dehydrogenase
H&E
haematoxylin and eosin
HMDS
hexamethyldisilazane
hMSC
human mesenchymal stem cell
HPRT1
hypoxanthine phosphoribosyl-transferase 1
MSCGM
Mesenchymal Stem Cells Growth Medium
PBS
phosphate buffered saline
PCNA
proliferating cell nuclear antigen
PPIA
peptidyl-prolyl isomerase-A (cyclophilin-A)
RUNX2
runt-related transcription factor 2
SEC
scaffold-exposed culture
SEM
scanning electron microscopy
SPARC
secreted protein acidic rich in cysteine (osteonectin)
SPP1
osteopontin
TGF
transforming growth factor
Cited by (0)
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These two authors equally contributed to the work.
© 2019 The Authors. Published by Elsevier B.V. on behalf of International Society of Differentiation.