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Comparison of Anaerobic Methane Oxidation in Different Sediment Habitats of Dianchi Lake

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Abstract

Anaerobic methane oxidation is a key process for methane reduction and nitrogen removal in eutrophic lakes. It is very important to know the distribution patterns of anaerobic methane oxidation activity and the related microbes in the typical plateau eutrophic lakes. Here, we aimed to characterize the anaerobic methane oxidation activity and to reveal the correlations to biological/non-biological factors along eutrophic gradients in Dianchi Lake. The anaerobic methane oxidation activity was analyzed by anaerobic incubation and gas chromatography. Candidatus Methylomirabilis was analyzed by Illumina Miseq 16S ribosomal RNA gene sequencing. The methane oxidation activities ranged from 1.71 to 5.07 μg/g day−1 in Dianchi Lake. These activities differed significantly among the four zones (p = 0.000), with more activity in the high eutrophication zone (Caohai). The relative abundance of Candidatus Methylomirabilis ranged from 84.86 to 98.13%, with no significant changes evident in the four research zones in Dianchi Lake. The anaerobic methane oxidation activity was significantly positively related to TN, NO3−-N, TC, and Candidatus Methylomirabilis abundance, and significantly negatively related to TP and pH. Thus, our study showed anaerobic methane oxidation occurred in Dianchi sediment and the activity of anaerobic methane oxidation was associated with the level of eutrophication.

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Funding

This study was financially supported by the National Natural Science Foundation of China (31700411 and 41663008), the Programs of Science and Technology Department Foundation of Yunnan Province (2018FD007, 2016FD014, and 2018 BC001), the Undergraduate Training Programs for Innovation and Entrepreneurship of Yunnan Province (201710673047), and the Yunnan Key Laboratory for Plateau Mountain Ecology and Restoration of Degraded Environments (2018DG005).

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Authors and Affiliations

  1. Institute for Ecological Research and Pollution Control of Plateau Lakes, School of Ecology and Environmental Science, Yunnan University, Kunming, 650091, China

    Wei Li, Yue Chen, Mingmo Luo, Chunyan Li, Yonggui Zhao & Bin Hu

  2. Yunnan Key Laboratory for Plateau Mountain Ecology and Restoration of Degraded Environments, Yunnan University, Kunming, 650091, China

    Wei Li, Yue Chen, Mingmo Luo, Yonggui Zhao & Bin Hu

  3. Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization & Ecological Restoration Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China

    Huai Chen

  4. Research Institute of Resource Insects, Chinese Academy of Forestry, Kunming, 650224, China

    Defeng Feng

Authors
  1. Wei Li
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  2. Yue Chen
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  3. Mingmo Luo
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  4. Chunyan Li
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  5. Yonggui Zhao
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  6. Huai Chen
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  7. Defeng Feng
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  8. Bin Hu
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Correspondence to Defeng Feng or Bin Hu.

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Li, W., Chen, Y., Luo, M. et al. Comparison of Anaerobic Methane Oxidation in Different Sediment Habitats of Dianchi Lake. Water Air Soil Pollut 231, 491 (2020). https://doi.org/10.1007/s11270-020-04868-5

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  • DOI: https://doi.org/10.1007/s11270-020-04868-5

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