Research articlesPreparation of magnetic microgels based on dextran for stimuli-responsive release of doxorubicin
Graphical abstract
Magnetic dextran microgels incorporated with Fe3O4 nanoparticles were prepared and a pH/magnetic field dual sensitive drug release behavior was demonstrated.
Section snippets
Introductions
Hydrogel nanocomposites, which are composed of polymeric hydrogel and organic nanoparticles embedded in the hydrogel matrix, have recently attracted interests by their unique inorganic/organic hybrid structure, improved mechanical properties and accelerated response as remote controlled biomaterials [1], [2]. Nanoparticles that can generate heat under specific stimuli, including the carbon nanotubes, gold and magnetic nanoparticles are widely used for the preparations of hydrogel
Materials
Dextran T40 (Mw: 40 000 Da), dextran T70 (Mw: 70 000 Da), ethylenediamine (AR, 98%), sodium periodate (NaIO4, AR, 99.5%), doxorubicin hydrochloride (DOX·HCl, 98%) and cyclohexane (AR, 99.5%) were purchased from Aladdin CO. Ltd. (Shanghai, China) and used as received. Iron(III) chloride hexahydrate (FeCl3·6H2O, reagent grade, 98%), iron(II) chloride tetrahydrate (FeCl2·4H2O, reagent grade, 98%), Span 80 (Sorbitan monooleate), Tween 80 and Brij L4 were purchased from Sigma Aldrich Chemical Co.
Characteristics of magnetic Fe3O4 nanoparticles
Magnetic Fe3O4 nanocrystal (also known as magnetite) is amongst the most widely used iron oxide nanoparticles for bio-applications, due to their excellent biocompatibility, biodegradability, and superparamagnetic properties as the size changed dramatically to smaller than 20 nm. In order to prepare magnetic hydrogels doped with Fe3O4 NPs via the physically blending method, water-soluble Fe3O4 NPs were synthesized via aqueous co-precipitation of Fe2+/Fe3+ salt using dextran as polymeric
Conclusions
A series of magnetic dextran microgels with various MNPs loading were conveniently prepared by physically blending of Fe3O4 NPs into dextran microgels formed via Schiff base reactions between aldehyded dextran and diamine in W/O inverse microemulsion. All the resulted magnetic microgels showed superparamagnetic behaviors and their magnetic properties were strongly affected by the size and content of Fe3O4 NPs. In vitro release behavior study demonstrated a magnetic field/pH dual sensitive DOX
CRediT authorship contribution statement
Lihua He: Methodology, Formal analysis, Investigation, Writing - original draft. Rong Zheng: Investigation. Jie Min: Investigation. Fulin Lu: Investigation. Changqiang Wu: Methodology. Yunfei Zhi: Investigation. Shaoyun Shan: Writing - review & editing. Hongying Su: Conceptualization, Validation, Supervision, Writing - review & editing, Project administration.
Declaration of Competing Interest
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Acknowledgements
This work was financially supported by the National Natural Science Foundation of China [51963013, 51503090].
References (29)
- et al.
Magnetic hydrogel nanocomposites for remote controlled pulsatile drug release
J. Control. Release
(2008) - et al.
Synthesis and characterization of magnetic dextran nanogel doped with iron oxide nanoparticles as magnetic resonance imaging probe
Int. J. Biol. Macromol.
(2019) - et al.
Recent advances in tough and self-healing nanocomposite hydrogels for shape morphing and soft actuators
Eur. Polym. J.
(2020) - et al.
Environment sensitive hydrogels for drug delivery applications
Eur. Polym. J.
(2019) - et al.
Preparation and characterization of thermosensitive organic–inorganic hybrid microgels with functional Fe3O4 nanoparticles as crosslinker
Polymer
(2011) - et al.
Insight on the periodate oxidation of dextran and its structural vicissitudes
Polymer
(2011) - et al.
Synthesis, characterization and MRI application of dextran-coated Fe3O4 magnetic nanoparticles
Biochem. Eng. J.
(2008) - et al.
Synthesis and surface engineering of iron oxide nanoparticles for biomedical applications
Biomaterials
(2005) - et al.
Synthesis and characterization of Schiff base contained dextran microgels in water-in-oil inverse microemulsion
Carbohydr. Polym.
(2016) - et al.
A novel magnetic MgFe2O4–MgTiO3 perovskite nanocomposite: rapid photo-degradation of toxic dyes under visible irradiation
Compos. Part B: Eng.
(2019)
Preparation of a new magnetic and photo-catalyst CoFe2O4–SrTiO3 perovskite nanocomposite for photo-degradation of toxic dyes under short time visible irradiation
Compos. Part B: Eng.
Facile preparation of pH/reduction dual-stimuli responsive dextran nanogel as environment-sensitive carrier of doxorubicin
Polymer
Nano-ferrosponges for controlled drug release
J. Control. Release
Dual and multi-stimuli responsive polymeric nanoparticles for programmed site-specific drug delivery
Biomaterials
Cited by (21)
Past, present and future of biomedical applications of dextran-based hydrogels: A review
2023, International Journal of Biological MacromoleculesMagnetic-responsive polysaccharide hydrogels as smart biomaterials: Synthesis, properties, and biomedical applications
2022, Carbohydrate PolymersCitation Excerpt :However, they are often functionalised to design smart polysaccharide hydrogels with improved properties and stimuli-responsiveness (Deng et al., 2018; Vashist et al., 2018). Smart polysaccharide hydrogels are biomaterials developed for responding to external stimuli such as pH, temperature, light, and magnetic field, that have received great attention in various biological fields (H. Ding, Li, et al., 2021; L. He et al., 2021; M. He et al., 2014; Hussain et al., 2019; Nisar et al., 2020; Qu et al., 2017). Among these stimuli, magnetic-responsiveness hydrogels have been widely studied, due to their ability to be controlled by an external magnetic field (EMF).
Construction of targeted delivery system for curcumin loaded on magnetic α-Fe<inf>2</inf>O<inf>3</inf>/Fe<inf>3</inf>O<inf>4</inf> heterogeneous nanotubes and its apoptosis mechanism on MCF-7 cell
2022, Biomaterials AdvancesCitation Excerpt :Compared with that in Fig. 5(b), the orange-red fluorescence in Fig. 5(c) completely disappeared, which indicated that the coating of liposomes increased the biocompatibility of nanomaterials, and of course, the toxicity of the bare materials themselves was also very low. Compared with groups of free curcumin (Fig. 5(d)) and α-Fe2O3/Fe3O4-CUR@LIP without MF (Fig. 5(e)), the globular nuclear chromatin and the strongest orange-red fluorescence in Fig. 5(f) indicated a large amount of cell death, revealing that the nanocarrier reinforced the killing influence on curcumin under action of MF [67,68]. The cytotoxicity efficacy of α-Fe2O3/Fe3O4-CUR@LIP group under magnetic field was stronger than that without magnetic field, which implied that magnetic field accelerated the internalization of cells and drug release.
Strategies for improving the safety and RNAi efficacy of noncovalent peptide/siRNA nanocomplexes
2022, Advances in Colloid and Interface ScienceCitation Excerpt :In tumor-targeted delivery systems, stimuli-responses and/or active targeting effects often happen in conjunction with passive targeting to successfully accumulate anti-cancer therapies to targeted sites and cells. In response to the microenvironmental differentiations, e.g., pH [192], redox [193], enzyme [194], and hypoxia [195], between normal and tumor tissues and in vitro stimuli like temperature [196], ultrasound [197], light [198], and magnetic fields [199], stimuli-responsive delivery systems could enrich nanoparticles and release their cargos at tumor tissues and cells, leading to the enhanced therapeutic index of medications [190,200]. The pH gradient between normal (pH 7.4) and tumor tissues (around pH 6.5) was caused by excess secretion of lactic acid and rapid growth of tumor cells.
Dextran-based micro and nanobiomaterials for drug delivery and biomedical applications
2022, Micro- and Nanoengineered Gum-Based Biomaterials for Drug Delivery and Biomedical ApplicationsEngineering nanocomposite hydrogels using dynamic bonds
2021, Acta BiomaterialiaCitation Excerpt :On the other hand, surface-functionalized NPs can behave as gelators to chemically crosslink the polymeric network of hydrogels (Scheme 1c) [46]. Nevertheless, some favorable features of biomaterials (e.g., self-healing, stimulus-responsiveness, shear-thinning and viscoelasticity) are still missing in NC hydrogels fabricated using covalent crosslinking [47–49]. NC hydrogels fabricated using dynamic bonds have been demonstrated to exhibit unique characteristics and dynamic features compared to traditional NC hydrogels.