Abstract
Fire-stimulated flowering and fire-stimulated resprouting are associated traits common in plants that evolved in fire-prone habitats, e.g., Vellozia pyrantha, an iconic plant that has economic potential and is endemic to a fully protected area. By combining a natural phenomenon that drives demography and in situ evolution, we tested if fire can be used as a novel technique for plant multiplication through vegetative bud activation in V. pyrantha. The basal parts of microplants cultivated for 60 days (5 to 6 cm tall) were exposed to a flame using a Bunsen burner for five periods controlled by a timer (2, 4, 6, 8, and 10 s) and compared to unburned plants (control). After 30 days, the number of new shoots was counted, and the length of the longest shoot produced per individual was measured. Adventitious shoot development started 7 days after the fire incidence. The number of shoots per individual and length of the longest shoot produced were significantly higher in the 6- to 10-s treatments. Fire was an efficient and cheap way to induce adventitious shoot formation. This new method could also be applied to other resprouter species from fire-prone environments in the world.
Key message
By combining a natural phenomenon that drives evolution with the bud stimulus expected, we found fire as an efficient and cheap way to induce buds.
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Acknowledgements
This study was partially financed by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) – Finance code 001, the Conselho Nacional de Desenvolvimento Científico e Tecnológico – CNPq (Grant Numbers 556820/2011-0, 302986/2016-2), and the Fundação de Amparo à Pesquisa do Estado da Bahia - FAPESB (Grant Number 26/2011). The English revision was financed by Fundação Grupo Boticário de Proteção à Natureza (FGBPN 1084_20162). We thank Rafael Oliveira for the comments and suggestions.
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BPSB, AAC and JRFS designed the study, AAC and BPSB wrote the manuscript, and BPSB, ALB and APPSL conducted the experiments in the laboratory and performed the data analyses.
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Communicated by Amita Bhattacharya.
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dos Santos Borges, B.P., Lima, A.P.P.S., Lima-Brito, A. et al. Fire as a novel technique to stimulate adventitious shoots in the laboratory. Plant Cell Tiss Organ Cult 143, 709–713 (2020). https://doi.org/10.1007/s11240-020-01933-z
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DOI: https://doi.org/10.1007/s11240-020-01933-z