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Associative Genetic Diversity of RoTat 1.2 VSG in Different Trypanosoma evansi Isolates

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Abstract

Background

Numerous phylogenetic markers have been tested over a period of time for delineating evolutionary history of haemoflagellate—Trypanosoma evansi.

Purpose

To find out the associative genetic diversity, within the various isolates of T. evansi across the globe, based on RoTat 1.2 VSG gene.

Methods

A total of 5 equine isolates of T. evansi from Northern India were characterized. PCR products were sequenced and sequences were compared with available sequences across India and world. Phylogenetic tree was constructed based on maximum parsimony (MP) method with the tree-bisection-regrafting (TBR) algorithm.

Results

Indian isolates formed multiple clades with two haplotypes. The present isolates showed 99.49–100.00% nucleotide homology within themselves. On broader line, Indian isolates were found to be closer to Egyptian isolates than the African counterparts. Few of the Indian isolates showed marked resemblance with a particular Egyptian isolate than with their Indian counter parts. Another remarkable finding is the close association of equine isolates from India with other equine isolates and their clear divergence from isolates of T. evansi affecting other hosts from India and abroad.

Conclusion

Vast genetic divergence was seen between the isolates suggesting of multiple distinct lineages of T. evansi amongst the Indian livestock. Interestingly, variations in sequences were seen based on the host range of isolates. The findings are very important from molecular evolutionary point of view.

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Acknowledgements

The authors are highly thankful to the Director Research, Dean CoVSc and Vice Chancellor, DUVASU, for the facilities provided. The authors also want to acknowledge the various funding agencies like Indian Council of Agricultural Research (ICAR) and Rashtriya Krishi Vikas Yojana (RKVY) for sanctioning various projects to the University for the procurement of instruments to carry out such work. The authors also acknowledge Incharge, Central Instrumentation Facility (CIF) for permitting the use of instruments required in the study.

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Authors and Affiliations

  1. Department of Parasitology, College of Veterinary Science and Animal Husbandry, U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura, 281001, India

    Ruchi Singh Gaur, Daya Shanker & Vikrant Sudan

  2. College of Biotechnology, College of Veterinary Science and Animal Husbandry, U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura, 281001, India

    Sanjhi Paliwal

  3. Department of Veterinary Clinical Medicine, College of Veterinary Science and Animal Husbandry, U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura, 281001, India

    Shanker Singh

  4. College of Veterinary Science and Animal Husbandry, Dharapura, Dholpur, India

    Ashutosh Jadaun

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  1. Ruchi Singh Gaur
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  2. Daya Shanker
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  3. Vikrant Sudan
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Correspondence to Vikrant Sudan.

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Gaur, R.S., Shanker, D., Sudan, V. et al. Associative Genetic Diversity of RoTat 1.2 VSG in Different Trypanosoma evansi Isolates. Acta Parasit. 66, 199–204 (2021). https://doi.org/10.1007/s11686-020-00273-4

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  • DOI: https://doi.org/10.1007/s11686-020-00273-4

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