Cascade signal amplification for sensitive detection of exosomes by integrating tyramide and surface-initiated enzymatic polymerization

Zhipeng Huang; Qiuyuan Lin; Bin Yang; Xin Ye; Hui Chen; Wenhao Weng; Jilie Kong

doi:10.1039/D0CC04881J

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Cascade signal amplification for sensitive detection of exosomes by integrating tyramide and surface-initiated enzymatic polymerization†

Zhipeng Huang,

^a Qiuyuan Lin,^a Bin Yang,^a Xin Ye,

^a Hui Chen,

*^a Wenhao Weng*^b and Jilie Kong*^a

Author affiliations

* Corresponding authors

^a Department of Chemistry, Fudan University, Shanghai 200438, China
E-mail: chenhui@fudan.edu.cn, wengwenhao2010@163.com, jlkong@fudan.edu.cn

^b Department of Clinical Laboratory, Yangpu Hospital, Tongji University School of Medicine, Shanghai, China

Abstract

A novel cascade signal amplification based on tyramide signal amplification (TSA) and surface-initiated enzymatic polymerization (SIEP) was first reported for the sensitive and template-free detection of colorectal cancer (CRC) exosomes. This assay exhibited 20.9-fold signal amplification with a low detection limit of 12.8 particles per μL. Furthermore, accurate and reproducible results were obtained for detecting exosomes in serum samples, suggesting its potential application in exosomes analysis and clinical diagnostics.

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Article information

DOI: https://doi.org/10.1039/D0CC04881J
Article type: Communication
Submitted: 16 Jul 2020
Accepted: 15 Sep 2020
First published: 16 Sep 2020

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Chem. Commun., 2020,56, 12793-12796

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Cascade signal amplification for sensitive detection of exosomes by integrating tyramide and surface-initiated enzymatic polymerization

Z. Huang, Q. Lin, B. Yang, X. Ye, H. Chen, W. Weng and J. Kong, Chem. Commun., 2020, 56, 12793 DOI: 10.1039/D0CC04881J

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Chemical Communications

Cascade signal amplification for sensitive detection of exosomes by integrating tyramide and surface-initiated enzymatic polymerization†

Abstract

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Cascade signal amplification for sensitive detection of exosomes by integrating tyramide and surface-initiated enzymatic polymerization

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