Abstract
In this research, ‘Pedro’ walnut variety (maternal parent) was open pollinated. Offspring genotypes were compared with their maternal parent in terms of morphological and molecular features. Leafing and leaf fall dates were measured as two phenological traits. Genetic differences were identified by molecular fingerprinting using twelve Inter Simple Repetitive Sequence Polymorphism (ISSR) primers. In some progenies, leafing and leaf fall occurred later than the maternal parent (‘Pedro’). Progenies were grouped according to their similarities with the maternal parent regarding leafing date. Specifically, 14.44% of the progenies were ‘Very close’ to the parent, 16.66% were ‘Close’, 30.00% ‘Far’ and 38.89% ‘Very far’. Regarding the leaf fall date, 7.78% of the progenies were ‘Very close’ to the parent, 28.88% were ‘Close’, 36.66% ‘Far’ and 26.67% ‘Very far’. A total of 43 bands were obtained in molecular analyses. These bands were largely polymorphic (95.34%). The average number of the alleles was 3.90, while the polymorphism information content varied between 0.14 and 0.91 among the genotypes. Genetic distances were tested according to the UPGMA (Unweighted Pair Group Method with Arithmetic Average) and by The Structure Analysis. The UPGMA dendrogram divided the genotypes into two main clusters and the similarity coefficient of the genotypes varied between 0.61 and 0.99. This study revealed that ‘Pedro’ can be used as an excellent parental variety in hybridization programs which prioritize late-leafing in walnut breeding. Furthermore, ISSR primers can be safely used for fingerprinting walnuts in general.
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References
Abedi B, Parvaneh T (2016) Study of correlations between horticultural traits and variables affecting kernel percentage of walnut (Juglans regia L.). J Nuts 7:35–44
Ahmed N, Mir JI, Mir RR, Rather NA, Rashid R, Wani SH, Sheikh MA (2012) SSR and RAPD analysis of genetic diversity in walnut (Juglans regia L.) genotypes from Jammu and Kashmir, India. Physiol Mol Biol Plants 18:149–160
Akcan S (2007) Kaman Cevizlerinde apomiksis özelliğinin moleküler yöntemlerle araştırılması. Yüksek Lisans Tezi. Adana Çukurova Üniversitesi Fen Bilimleri Enstitüsü Adana. 93s
Amiri R, Vahdati K, Mohsenipoor S, Mozaffari MR, Leslie C (2010) Correlations between some horticultural traits in walnut. HortScience 45:1690–1694
Anderberg MR (1973) Cluster analysis for applications (No. OAS-TR-73-9). Office of the assistant for study support kirtland AFB n mex
Anonymous (2020) http://taylor0.biology.ucla.edu/structureHarvester/
Arab MM, Marrano A, Abdollahi-Arpanahi R, Leslie CA, Cheng H, Neale DB, Vahdati K (2019a) Combining phenotype, genotype and environment to uncover genetic components underlying water use efficiency in persian walnut. J Exp Bot 71:1107–1127
Arab MM, Marrano A, Abdollahi-Arpanahi R, Leslie CA, Askari H, Neale DB, Vahdati K (2019b) Genome-wide patterns of population structure and association mapping of nut-related traits in persian walnut populations from Iran using the Axiom J regia 700K SNP array. Sci Rep 9:6376
Arzani K, Mansouri-Ardakan H, Vezvaei A, Roozban MR (2008) Morphological variation among persian walnut (Juglans regia) genotypes from central Iran. N Zeal J Crop Hortic Sci 36:159–168
Bardak A (2012) Lif ve hav oluşumuyla ilişkili DNA markörlerinin pamuk (Gossypium hirsutum L.) genomunda haritalanması ve QTL analizi. Doktora Tezi. Kahramanmaraş Sütçü İmam Üniversitesi Fen Bilimleri Enstitüsü. Kahramanmaraş. 141s
Bardak A, Bölek Y (2012) Genetic diversity of diploid and tetraploid cottons determined by SSR and ISSR markers. Turk J Field Crops 17:139–144
Bou Abdallah I, Baatour O, Mechrgui K, Herchi W, Albouchi A, Chalghoum A, Boukhchina S (2016) Essential oil composition of walnut tree (Juglans regia L.) leaves from Tunisia. J Essent Oil Res 28:545–550
Christopoulos MV, Rouskas D, Tsantili E, Bebeli PJ (2010) Germplasm diversity and genetic relationships among walnut (Juglans regia L.) varieties and greek local selections revealed by inter-simple sequence repeat (ISSR) markers. Sci Hortic 125:584–592
Cosmulescu S, Stefanescu D (2018) Morphological variation among Persian walnut (Juglans regia) genotypes within the population and depending on climatic year. Sci Hortic 242:20–24
Cosmulescu S, Trandafir I (2011) Variation of phenols content in walnut (Juglans regia L.). South West J Hortic Biol Environ 2:25–33
Doğan Y (2006) Bazı ceviz (Juglans regia L.) çeşit ve genotypelerinin moleküler markör teknikleri ile karakterizasyonu. Yüksek Lisans Tezi. Çukurova Üniversitesi Fen Bilimleri Enstitüsü. Adana. 83s
Doğan Y, Kafkas S, Sütyemez M, Akça Y, Türemiş N (2014) Assessment and characterization of genetic relationships of walnut (Juglans regia L.) genotypes by three types of molecular markers. Sci Hortic 168:81–87
Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15
Earl DA, VonHoldt BM (2012) STRUCTUR E HARVESTER: a website and program for visualizing STRUCTURE output and implementing the Evanno method. Conserv Genet Resour 4:359–361
Ebrahimi A, Vahdati K (2006) Improved success of persian walnut grafting under environmentally controlled conditions. Int J Fruit Sci 6:3–12
Ebrahimi A, Fatahi MM, Zamani ZA, Vahdati K (2010) An investigation on genetic diversity of 608 Persian walnut accessions for screening of some genotypes of superior traits. Iran J Hortic Sci 40:83–94
Ebrahimi A, Fatahi R, Zamani Z (2011) Analysis of genetic diversity among some persian walnut genotypes (Juglans regia L.) using morphological traits and SSRs markers. Sci Hortic 130:146–151
Ebrahimi A, Khadivi-Khub A, Nosrati Z, Karimi R (2015) Identification of superior walnut (Juglans regia) genotypes with late leafing and high kernel quality in Iran. Sci Hortic 193:195–201
Ercisli S, Sayinci B, Kara M, Yildiz C, Ozturk I (2012) Determination of size and shape features of walnut (Juglans regia L.) cultivars using image processing. Sci Hortic 133:47–55
Erdoğan V, Aygün A (2016) Ceviz (Juglans regia L.) çeşitlerinin RAPD tekniği ile genetik tanımlanması. Bahçe 45:919–924
Eser E, Topçu H, Kefayatı S, Sütyemez M, Islam MR, Kafkas S (2019) Highly polymorphic novel simple sequence repeat markers from class repeats in walnut Juglans regia L. Turk J Agric For 43:174–183
Evanno G, Regnaut S, Goudet J (2005) Detecting the number of clusters of individuals using the software structure: a simulation study. Mol Ecol 14:2611–2620
Ghasemi M, Arzani K, Hassani D (2012) Evaluation and identification of walnut (Juglans regia L.) genotypes in Markazi province of Iran. Crop Breed J 2:119–124
Golein B, Bigonah M, Azadvar M, Golmohammadi M (2012) Analysis of genetic relationship between ‘Bakraee’ (Citrus sp.) and some known Citrus genotypes through SSR and PCR–RFLP markers. Sci Hortic 148:147–153
Hajnajari H, Chashnidel B, Vahdati K, Ebrahimi M, Agheli SO (2012) Evaluation of morphological markers efficiency in screening of cross combinations progenies during apple juvenile phase. HortScience 13:11–30
Hassankhah A, Vahdati K, Rahemi M, Sarikhani Khorami S (2017) Persian walnut phenology: effect of chilling and heat requirements on bud break and flowering date. Int J Hort Sci Tech 4:259–271
Hussain I, Sulatan A, Shinwari ZK, Raza G, Ahmed K (2016) Genetic diversity based on morphological traits in walnut (Juglans regia L.) landraces from Karakoram Region-I. Pak J Bot 48:653–659
Ikhsan AS, Topcu H, Sutyemez M, Kafkas S (2016) Novel 307 polymorphic SSR markers from BAC-end sequences in walnut (Juglans regia L.): effects of motif types and repeat lengths on polymorphism and genetic diversity. Sci Hortic 213:1–4
Ipek M, Arıkan Ş, Pırlak L, Eşitken A (2019) Phenological, morphological and molecular characterization of some promising walnut (Juglans regia L.) genotypes in Konya. Erwerbs-Obstbau 61:149–156
IPGRI (1994) Descriptors for walnut (Juglans spp.). International Plant Genetic Resources Institute, Rome, Italy
Karimi R, Ershadi A, Vahdati K, Woeste K (2010) Molecular characterization of persian walnut populations in Iran with microsatellite markers. HortScience 45:1403–1406
Khadivi-Khub A, Ebrahimi A, Sheibani F, Esmaeili A (2015) Phenological and pomological characterization of persian walnut to select promising trees. Euphytica 205:557–567
Laborda PR, Oliveira KM, Garcia AA, Paterniani ME, De Souza AP (2005) Tropical maize germplasm: what can we say about its genetic diversity in the light of moleculer markers. Theor Appl Genet 111:1288–1299
Mahmoodi R, Hassani D, Amiri ME, Jaffaraghaei M (2016) Phenological and pomological characteristics of five promised walnut genotypes in Karaj, Iran. J Nuts 7:1–8
Malvolti ME, Pollegioni P, Bertani A, Mapelli S, Cannata F (2010) Juglans regia provenance research by molecular, morphological and biochemical markers: a case study in Italy. Biorem Biodiv Bioavail 4:84–92
Najafi F, Mardi M, Fakheri B, Pirseyedi SM, Mehdinejad N, Farsi M (2014) Isolation and characterization of novel microsatellite markers in walnut (Juglans regia L.). Am J Plant Sci 5:409–415
Nei M (1972) Genetic distance between populations. Am Nat 106:283–292
Norouzi R, Heidari S, Asgari-Sarcheshmeh MA, Shahi-Garahlar A (2013) Estimation of phenotypical and morphological differentiation among some selected Persian walnut (Juglans regia L.) accessions. Intl J Agron Plant Prod 4:2438–2445
Özcan A, Sütyemez M (2017) Bazı ceviz (Juglans regia L.) çeşitlerinin çimlenme ve çöğür anaçlık gelişme performanslarının belirlenmesi. KSÜ Doğa Bil Derg 20:75–79
Pollegioni P, Bartoli S, Cannata F, Malvolti ME (2003) Genetic differentiation of four Italian walnut (Juglans regia L.) varieties by inter simple sequence repeat (ISSR). J Genetics Breeding 57:231–240
Pollegioni P, Woeste K, Binbagı A, Mugnozza G, Scarascia Malvolti ME (2008) Characteristics of Juglans nigra (L.), Juglans regia (L.) and Juglans × Intermediate (Carr.) SSR Markers: a case study in Italy. Silvae Genetica 58:68–78
Pop IF, Vicol AC, Botu M, Raica PA, Vahdati K, Pamfil D (2013) Relationships of walnut cultivars in a germplasm collection: comparative analysis of phenotypic and molecular data. Sci Hortic 153:124–135
Potter D, Gao F, Aiello G, Leslie C, McGranahan GH (2002) Inter simple sequence repeat markers for fingerprinting and determining genetic relationships of walnut (Juglans regia) varieties. J Am Soc Hort Sci 127:75–81
Pritchard JK, Stephens M, Donnelly P (2000) Inference of population structure using multilocus genotype data. Genetics 155:945–959
Reddy MP, Sarla N, Siddiq A (2002) Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding. Euphytica 128:9–17
Rezaei Z, Khadivi A, ValizadehKaji B, Abbasifar A (2018) The selection of superior walnut (Juglans regia L.) genotypes as revealed by morphological characterization. Euphytica 214:69. https://doi.org/10.1007/s10681-018-2153-z
Ribeiro FADL, Mantovani GA, Poppi RJ, Rosário FFD, Bezerra MCM, Bastos ALM, Melo VLAD (2013) PCA: Uma ferramenta para identificação de traçadores químicos para água de formação e água de injeção associadas à produção de petróleo. Quim Nova 36:1281–1287
Rohlf FJ (1998) NTSYSpc numerical taxonomy and multivariate analysis system version 2.0 user guide
Sadat Hosseini M, Vahdati K, Leslie CA (2019) Germination of Persian walnut somatic embryos and evaluation of their genetic stability by ISSR fingerprinting and flow cytometry. HortScience 54:1576–1580
Sütyemez M (2018) Bitki ıslahı ders notları (Yayınlanmamış). Kahramanmaraş Sütçü İmam Üniversitesi Ziraat Fakültesi Bahçe Bitkileri Bölümü BBB302
Sütyemez M, Kaşka N (2002) Bazı yerli ve yabancı ceviz (Juglans regia L.) çeşitlerinin Kahramanmaraş ekolojisine adaptasyonu. KSÜ Fen ve Mühendislik Dergisi 5:148–158
Topçu H, Ikhsan AS, Sütyemez M, Çoban N, Güney M, Kafkas S (2015) Development of 185 polymorphic simple sequence repeat SSR markers from walnut Juglans regia L. Sci Hortic 194:160–167
Ünal B (2011) Niksar ekolojik koşullarında bazı yerli ve yabancı ceviz çeşitlerinin adaptasyon yeteneklerinin belirlenmesi. Yüksek Lisans Tezi. Gaziosmanpaşa Üniversitesi Fen Bilimleri Enstitüsü. Tokat. 59s
Vahdati K, Pourtaklu SM, Karimi R, Barzehkar R, Amiri R, Mozaffari M, Woeste K (2015) Genetic diversity and gene flow of some persian walnut populations in southeast of Iran revealed by SSR markers. Plant Syst Evol 301:691–699
Vahdati K, Arab MM, Sarikhani Khorami S, Sadat Hosseini M, Leslie CA, Brown PJ (2019) Advances in walnut (Juglans regia L.) breeding strategies, Chapter 13. In: Al-Khayri JM, Jain SM, Johnson DV (eds) Advances in plant breeding strategies, vol 4. Nut and Beverage Crops, Springer, Cham
Woodworth RH (1930) Meiosis of microsporogenesis in the Juglandaceae. Am J Bot 9:863–869
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Özcan, A., Sütyemez, M., Nar, A. et al. Similarities of leafing and leaf fall date of ‘Pedro’ walnut variety with its progenies in breeding programs. Physiol Mol Biol Plants 26, 1945–1959 (2020). https://doi.org/10.1007/s12298-020-00872-5
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DOI: https://doi.org/10.1007/s12298-020-00872-5