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A novel aminopeptidase with potential debittering properties in casein and soybean protein hydrolysates

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Abstract

A new aminopeptidase (An-APa) was identified and biochemically characterized from Aspergillus niger CICIM F0215. It had maximal activity at 40 °C and pH 7.0 and exhibited a broad substrate specificity both on hydrophilic and hydrophobic amino acid residues at N-terminals. With An-APa hydrolysis for 1 h, the casein-pepsin and soybean protein isolates (SPI)-pepsin hydrolysates released both hydrophilic and hydrophobic amino acids and the hydrophobic amino acids having Q values (degree of hydrophobicity) greater than 1500 cal/mol were remarkably released. Leu, Ile, Phe, Tyr, Trp, Pro, Val and Lys in the casein hydrolysate after treatment with An-APa increased 18.61, 0.84, 11.35, 13.18, 3.34, 6.30, 7.46, and 8.19 mg/100 mL, respectively, and 19.72, 1.47, 18.37, 11.72, 4.61, 4.10, 8.13, and 5.85 mg/100 mL, respectively, in the SPI hydrolysate. Both accounted for 65.0% and 64.4% of total released free amino acids from casein and SPI hydrolysates, respectively. This indicated that An-APa could be potentially applicable in debittering protein hydrolysates.

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Acknowledgements

This work was kindly supported by the Intergovernmental International Scientific and Technological Innovation Cooperation program, MOST, China (Grant No.: 2018YFE0100400), the Raising Program of Innovation Team for Tianjin Universities, Tianjin, China (Grant No.: TD13-5009), and the Tianjin Key R&D Projects Supported by Science and Technology, TMSTB, Tianjin, China (Grant No.: 19YFZCSN00560).

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Authors and Affiliations

  1. College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China

    Peng Song & Lei Cheng

  2. Department of Biological Chemical Engineering, College of Chemical Engineering and Materials Science, Tianjin University of Science and Technology, Tianjin, 300457, China

    Peng Song, Lei Cheng, Kangming Tian, Meng Zhang, Dandan Niu & Zheng-Xiang Wang

  3. Department of Biotechnology and Food Technology, Faculty of Applied Sciences, Durban University of Technology, P. O. Box 1334, Durban, 4001, South Africa

    Suren Singh

  4. Department of Microbiology, University of Stellenbosch, Private Bag X1, Matieland, 7602, South Africa

    Bernard Prior

  5. Agricultural Research Council, Biotechnology Platform, Private Bag X5, Onderstepoort, 0110, South Africa

    Nokuthula Peace Mchunu

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  1. Peng Song
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Song, P., Cheng, L., Tian, K. et al. A novel aminopeptidase with potential debittering properties in casein and soybean protein hydrolysates. Food Sci Biotechnol 29, 1491–1499 (2020). https://doi.org/10.1007/s10068-020-00813-8

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