Abstract
Cadmium (Cd) is a widespread toxic occupational and environmental pollutant, and its effect on lipid metabolism dysregulation has been observed. In this study, we utilized two-dimensional electrophoresis (2-DE) and mass spectrometry (MS) technologies to explore changes in the blood plasma proteins of mice exposed to Cd. From the 2-DE, 8 protein spots were screened in response to Cd exposure, and the identities of these proteins were revealed by MALDI-TOF MS. Western blotting was applied to analyze the expression of the apolipoproteins in both plasma and liver, which were consistent with Cd-induced dyslipidemia of their composed lipid. Moreover, the Cd-induced apolipoprotein ApoE upregulation was due to inhibition of autopahgic flux in the Cd exposed mice. It was further observed from the mouse liver that Cd reduced the expression of the lipid uptake receptor low-density lipoprotein receptor (LDLR), which might be responsible for the coordinated elevation in blood triglycerides and abnormal apolipoproteins. This study may provide a new insight into the mechanism of Cd-induced dyslipidemia and the risk of cardiovascular diseases.
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Abbreviations
- 2-DE:
-
Two-dimensional electrophoresis
- ABCA1:
-
ATP-binding cassette transporter A1
- AD:
-
Alzheimer’s disease
- Apcs:
-
Amyloid P-component precursor
- ApoA1p:
-
Apolipoprotein A-I precursor
- ApoA2:
-
Apolipoprotein A-II
- ApoA4:
-
Apolipoprotein A-IV
- ApoC3p:
-
Apolipoprotein C-III precursor
- ApoE:
-
Apolipoprotein E
- Cd:
-
Cadmium
- FFA:
-
Free (non-esterified) fatty acids
- HDL-C:
-
High density lipoprotein-cholesterol
- IEF:
-
Isoelectric focusing
- LDL-C:
-
Low density lipoprotein-cholesterol
- LDLR:
-
Low-density lipoprotein receptor
- MTTP:
-
Microsomal triglyceride transfer protein
- MS:
-
Mass spectrometry
- PZP:
-
Pregnancy zone protein
- PL:
-
Phospholipids
- RBP:
-
Retinol-binding protein
- TC:
-
Total cholesterol
- TG:
-
Total triglycerides
- VLDL-C:
-
Very low density lipoprotein-cholesterol
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Acknowledgements
This work was supported by the National Natural Science Foundation of China (31600952 and 31271272) and the Start-Up Research Funding of Jiangsu University for Distinguished Scholars (5501330001). We thank the animal house staff for their care of the animals, and Ms. Ye Pan for technical assistance with the 2-D gel analysis.
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HL, YW, ZR, XJ and SD performed the research; JG and HS designed the research study and contributed essential reagents or tools; HL, YW, ZR, XJ, XZ, SD, YZ, JG and HS analyzed and interpreted the data; HL, YW, ZR, XJ, Frank, SD, JG and HS wrote the manuscript. All authors reviewed the manuscript.
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Liu, H., Wang, Y., Ren, Z. et al. Dietary cadmium exposure causes elevation of blood ApoE with triglyceride level in mice. Biometals 33, 241–254 (2020). https://doi.org/10.1007/s10534-020-00247-z
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DOI: https://doi.org/10.1007/s10534-020-00247-z