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First Molecular Characterization and Seasonality of Larvae of Trichostrongylid Nematodes in Arrested Development in the Abomasum of Iranian Naturally Infected Sheep

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Abstract

Purpose

The aim of this study was to determine molecularly the species of larvae of trichostrongylid nematodes in arrested development in the abomasum of sheep from Iran and their seasonality.

Methods

A total of 240 sheep abomasa were randomly collected at different seasons from Ilam abattoir in 2017–2018. The adult nematodes were isolated and identified. The mucosa of the abomasum was removed and digested using a pepsin solution. DNA from different parts of the abomasum was extracted using a SinaPureTM kit. Polymerase chain reaction (PCR) amplification was performed; the ITS2-rRNA gene was amplified using specific primers. Amplified PCR products were purified and sequenced by Bioneer Corporation (South Korea). The obtained ITS2 sequences were aligned using BLAST analysis and submitted to GenBank.

Results

Morphologically, five species of adult nematodes of four genera including Haemonchus contortus, Marshallagia marshalli, Marshallagia occidentalis, Parabronema skrjabini, and Teladorsagia circumcincta were identified. Marshallagia marshalli (65.4%, 120.2 ± 28.5) had the highest prevalence during autumn. Molecularly, arrested development of larvae of M. marshalli and T. circumcincta were demonstrated in mucosa of the abomasum in autumn and early winter using PCR of the ITS-2 region of rRNA.

Conclusion

The results evidenced the first molecular characterization of the larvae of trichostrongylid nematodes from abomasum of sheep from Iran.

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Acknowledgements

The authors would like to thank the technical assistance of the members at Parasitology Division of Urmia and Ilam Faculties of Veterinary Medicine, Iran.

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Authors and Affiliations

  1. Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Nazlu Campus, POBox: 57153-1177, Urmia, Iran

    Maryam Nazarbeigy & Mohammad Yakhchali

  2. Department of Microbiology, Faculty of Veterinary Sciences, Ilam University, Ilam, Iran

    Fazel Pourahmad

Authors
  1. Maryam Nazarbeigy
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  2. Mohammad Yakhchali
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  3. Fazel Pourahmad
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Correspondence to Mohammad Yakhchali.

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Nazarbeigy, M., Yakhchali, M. & Pourahmad, F. First Molecular Characterization and Seasonality of Larvae of Trichostrongylid Nematodes in Arrested Development in the Abomasum of Iranian Naturally Infected Sheep. Acta Parasit. 66, 193–198 (2021). https://doi.org/10.1007/s11686-020-00274-3

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