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Mapping QTL for important seed traits in an interspecific F2 population of pigeonpea

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Abstract

Seed traits present important breeding targets for enhancing grain yield and quality in various grain legume crops including pigeonpea. The present study reports significant genetic variation for six seed traits including seed length (SL), seed width (SW), seed thickness (ST), seed weight (SWT), electrical conductivity (EC) and water uptake (WU) among Cajanus cajan (L.) Millspaugh acc. ICPL 20340 and Cajanus scarabaeoides (L.) Thouars acc. ICP 15739 and an F2 population derived from this interspecific cross. Maximum phenotypic values recorded for the F2 population were higher than observed in the parent ICPL 20340 [F2 max vs ICPL 20340: SW (7.05 vs 5.38), ST (4.63 vs 4.51), EC (65.17 vs 9.72), WU (213.17 vs 109.5)], which suggested contribution of positive alleles from the wild parent, ICP 15739. Concurrently, to identify the QTL controlling these seed traits, we assayed two parents and 94 F2 individuals with 113 polymorphic simple sequence repeat (SSR) markers. In the F2 population, 98 of the 113 SSRs showed Mendelian segregation ratio 1:2:1, whereas significant deviations were observed for 15 SSRs with their χ2 values ranging between 6.26 and 20.62. A partial genetic linkage map comprising 83 SSR loci was constructed. QTL analysis identified 15 marker-trait associations (MTAs) for seed traits on four linkage groups i.e. LG01, LG02, LG04 and LG05. Phenotypic variations (PVs) explained by these QTL ranged from 4.4 (WU) to 19.91% (EC). These genomic regions contributing significantly towards observed variability of seed traits would serve as potential candidates for future research that aims to improve seed traits in pigeonpea.

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Acknowledgements

Financial support from Indian Council of Agricultural Research (ICAR), New Delhi is gratefully acknowledged. Dr C.V. Sameer Kumar is highly acknowledged for providing seeds of ICPL 20340 and ICP 15739.

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  1. Rintu Jha

    Present address: Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing, 100081, China

Authors and Affiliations

  1. Crop Improvement Division, ICAR-Indian Institute of Pulses Research (IIPR), Kanpur, 208024, India

    Abhishek Bohra, Rintu Jha, Amrit Lamichaney, Uday C. Jha, S. J. Satheesh Naik, Dibendu Datta, Alok K. Maurya, Abha Tiwari, Vivekanand Yadav, Farindra Singh, Indra P. Singh & Narendra P. Singh

  2. ICAR-Indian Agricultural Statistics Research Institute (ICAR-IASRI), New Delhi, 110012, India

    Deepak Singh

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  1. Abhishek Bohra
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Contributions

AB conceived the idea, planned experiments and wrote manuscript with RJ, UCJ and NPS. RJ, AT performed genotyping experiments. RJ performed QTL analysis. AL along with AKM, VY recorded measurements related to seed traits. AB, RJ, DS analyzed the data. FS, IPS and DD helped in developing mapping population and interpretation of results. All authors read and approved the final manuscript.

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Correspondence to Abhishek Bohra.

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Bohra, A., Jha, R., Lamichaney, A. et al. Mapping QTL for important seed traits in an interspecific F2 population of pigeonpea. 3 Biotech 10, 434 (2020). https://doi.org/10.1007/s13205-020-02423-x

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