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The use of thin-layer chromatography in the assessment of the quality of lutein-containing dietary supplements

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Abstract

Bivariant multiple development thin-layer chromatography technique (BMD–TLC) along with high-performance liquid chromatography–diode array detection–electrospray ionization–mass spectrometry (HPLC–DAD–ESI–MS) analysis was used in determination of lutein or lutein mixed with zeaxanthin in eight dietary supplements. The developed two-step TLC separation procedure combined purification, compaction of samples and separation of the analyzed compounds what significantly shortened and simplified samples preparation. Qualitative analysis was based on co-chromatography with reference substances and HPLC–DAD–ESI–MS analysis. It was revealed that three of eight dietary supplements did not contain lutein. In turn, quantitative analysis with the use of developed TLC conditions along with densitometry showed that the amount of lutein or its mixture with zeaxanthin in the others differed from that claimed by producers.

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Acknowledgements

This work was supported by the Ministry of Science and Higher Education Republic of Poland from the quality-promoting subsidy under the Leading National Research Centre (KNOW) programme 2012 – 2017.

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Authors and Affiliations

  1. Department of Pharmacognosy with Medicinal Plants Garden, Medical University of Gdańsk, Gen. J. Haller Str. 107, 80-416, Gdańsk, Poland

    Piotr Migas, Natalia Stempka & Mirosława Krauze-Baranowska

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  1. Piotr Migas
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  2. Natalia Stempka
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  3. Mirosława Krauze-Baranowska
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Correspondence to Piotr Migas.

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Migas, P., Stempka, N. & Krauze-Baranowska, M. The use of thin-layer chromatography in the assessment of the quality of lutein-containing dietary supplements. JPC-J Planar Chromat 33, 11–18 (2020). https://doi.org/10.1007/s00764-019-00001-3

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  • DOI: https://doi.org/10.1007/s00764-019-00001-3

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