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Influence of light spectra and elicitors on growth and ascaridole content using in vitro cultures of Dysphania ambrosioides L.

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Abstract

Dysphania ambrosioides L. is a medicinal plant with anti-helmintic potential. The aim of this study was to evaluate separately the effect of light spectra and elicitors on Dysphania ambrosioides growth and volatile constituents in vitro. Thus, plantlets were first cultured under blue (B), red (R), white, combinations of B:R (1:1, 2:1, 1:2) from LEDs and fluorescent lamps. Secondly, nodal segments were inoculated in the medium supplemented with chitosan (0, 50, 100, 150, and 200 mg L− 1) and salicylic acid (0, 3, 6, 9, and 12 mg L− 1). After 40 days of cultivation, the growth parameters and chemical composition of volatile constituents were evaluated. The light spectra significantly influenced in vitro growth of D. ambrosioides. The best growth occured using white LED or a blue:red combination of 2:1. It was also observed that the blue LEDs inhibited the synthesis of Z-ascaridole, while fluorescent light promoted a greater conversion of α-terpinene into ascaridole. The elicitors, chitosan and salicylic acid had a negative effect on the growth of nodal segments. However, the highest Z-ascaridole content was obtained at 50 to 100 mg L− 1 of chitosan and with 6 to 9 mg L− 1 of salicylic acid. The present study demonstrates that shoots regenerated from nodal segments exposed to different light spectra or on MS medium containing chitosan and salicylic acid can exhibit an altered growth and increased volatile constituents of interest.

Key message

Elicitors and different wavelengths provided by LEDs and fluorescent lamp were developed for Dysphania ambrosioides, altering growth and increase volatile constituents of interest.

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Abbreviations

GC/MS:

Gas chromatography/mass spectrometry

LED:

Light emitting diodes

R:

Red

B:

Blue

MS:

Murashige and Skoog medium

PPFD:

Photosynthetic photon flux density

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Acknowledgements

This work was financed by the Minas Gerais State Research Foundation (FAPEMIG—Fundação de Pesquisa do Estado de Minas Gerais), National Council for Scientific and Technological Development (CNPq—Conselho Nacional de Desenvolvimento Científico e Tecnológico), and the Coordination for the Improvement of Higher Education Personnel (CAPES—Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES—Finance Code 001).

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Authors and Affiliations

  1. Laboratory of Plant Tissue Culture and Medicinal Plants, Department of Agriculture, Federal University of Lavras, Lavras, 37200-000, Brazil

    Alexandre Alves de Carvalho, Alan da Cunha Honorato, Tainá Teixeira Rocha, Sâmia Torres Silva & José Eduardo Brasil Pereira Pinto

  2. Laboratory of Phytochemistry and Medicinal Plants, Agronomy Department, Federal University of Lavras, Lavras, 37200-000, Brazil

    Suzan Kelly Vilela Bertolucci

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  1. Alexandre Alves de Carvalho
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Contributions

AAC and JEBPP conceived the study, designed the experiments; AAC, SKVB, ACH, TTR, STS, and JEBPP performed experiments, analyzed data; AAC and JEBPP wrote the manuscript. All authors read and approved the manuscript.

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Correspondence to José Eduardo Brasil Pereira Pinto.

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Communicated by Konstantin V. Kiselev.

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de Carvalho, A.A., Bertolucci, S.K.V., Honorato, A.d. et al. Influence of light spectra and elicitors on growth and ascaridole content using in vitro cultures of Dysphania ambrosioides L.. Plant Cell Tiss Organ Cult 143, 277–290 (2020). https://doi.org/10.1007/s11240-020-01892-5

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  • DOI: https://doi.org/10.1007/s11240-020-01892-5

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