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LncRNA ADAMTS9-AS1, as prognostic marker, promotes cell proliferation and EMT in colorectal cancer

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Abstract

The long non‐coding RNA antisense 1 ADAMTS9-AS1 has been reported to predict the survival in several tumors, including bladder cancer and breast cancer. However, the clinical significance and biological behaviors of ADAMTS9-AS1 in colorectal cancer (CRC) have not been reported yet. In this study, the expression of ADAMTS9-AS1 was measured in CRC tissues and cell lines using quantitative real-time PCR analysis. The clinical significance of ADAMTS9-AS1 was evaluated with Chi-squared test, Kaplan–Meier method and Cox regression analysis in CRC patients. CCK8 assay, colony formation assay, flow cytometry and transwell assay were used to explore the biological function of ADAMTS9-AS1 knockdown in CRC cell lines (SW1116 and HT29). We further explore the role of ADAMTS9-AS1 in vivo though xenograft tumor assay. Our data showed that ADAMTS9-AS1 expression level was significantly up-regulated in CRC tissues and cell lines compared with corresponding controls. High ADAMTS9-AS1 level was associated with TNM stage, lymph node invasion and worse survival prognosis. Depletion of ADAMTS9-AS1 significantly suppressed cell proliferation, G1/S transition, migration and invasion, as well as suppressed CDK4/Cyclin D1 and epithelial–mesenchymal transition (EMT). To sum up, these findings illustrated that ADAMTS9-AS1 might be a promising therapeutic target and prognostic factor for CRC.

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Abbreviations

CRC:

Colorectal cancer

lncRNAs:

Long non-coding RNAs

EMT:

Epithelial to mesenchymal transition

DMEM:

Dulbecco’s modified Eagle’s medium

FBS:

Fetal bovine serum

NC:

Negative control

CCK-8:

Cell Counting Kit-8

PVDF:

Polyvinylidene fluoride

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Funding

This work was funded by 2019 Provincial Key Laboratory of Medical Physics and Technology Safety and Security Open Fund (no. LMPT201908).

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Author notes

  1. Wanjing Chen and Qian Tu contributed equally to this work.

Authors and Affiliations

  1. Department of General Surgery, Economic and Technological Development District, The Second Hospital of Anhui Medical University, No.678 Furong Road, Hefei, Anhui, China

    Wanjing Chen, Qian Tu, Liang Yu, Yanyan Xu, Gang Yu, Benli Jia, Yunsheng Cheng & Yong Wang

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  1. Wanjing Chen
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  2. Qian Tu
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  3. Liang Yu
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  4. Yanyan Xu
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  5. Gang Yu
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  6. Benli Jia
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  7. Yunsheng Cheng
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  8. Yong Wang
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Corresponding authors

Correspondence to Yunsheng Cheng or Yong Wang.

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Ethics approval and consent to participate

The present study was conducted in accordance with the Declaration of Helsinki and approved by the Ethics Committee of The Second Hospital of Anhui Medical University (Approval number: AM3-289; 2015.10.15, Anhui, China).

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Chen, W., Tu, Q., Yu, L. et al. LncRNA ADAMTS9-AS1, as prognostic marker, promotes cell proliferation and EMT in colorectal cancer. Human Cell 33, 1133–1141 (2020). https://doi.org/10.1007/s13577-020-00388-w

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  • DOI: https://doi.org/10.1007/s13577-020-00388-w

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