Abstract
Circular RNAs (circRNAs) are a new class of RNAs that play an important role in the development of various tumors. However, the expression profile and biological function of circRNAs in osteosarcoma (OS) progression remain unclear. OS-related circRNA expression profiles from the GEO database (GSE96964) were downloaded to identify differentially expressed circRNAs between OS and normal tissues. We identified one upregulated circRNA (Circ-XPR1), and RT-PCR was performed to further confirm the expression abundance in OS tissue. Circ-XPR1 was closely related to overall survival and disease-free survival of OS patients. Knockdown of Circ-XPR1 significantly reduced the proliferation of OS cells. Gain- and loss-of-function studies showed that Circ-XPR1 promoted OS cell proliferation by sponging miR-214-5p to regulate DDX5 expression. Our findings suggested that Circ-XPR1 regulates OS cell proliferation by sponging miR-214-5p to regulate DDX5 expression. Therefore, the Circ-XPR1/miR-214-5p/DDX5 axis may serve as a potential therapeutically relevant target for OS.
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XM and SG performed the majority of experiments and analyzed the data; LG and GL performed the molecular investigations; LH and GL designed and coordinated the research; XM and SG wrote the full texts.
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The specimens of osteosarcoma and adjacent tissues were collected from the patients who were diagnosed with osteosarcoma in Henan Provincial People’s Hospital. The informed consents for each patient were obtained and the procedures were approved by the Ethics Committee of Henan Provincial People’s Hospital (Approval no. 2020071203).
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13577_2020_412_MOESM1_ESM.tif
Supplement S1 Comparison of XPR1 expression in OS and control groups. (A) GSE12865 was used to assess the expression of XPR1 in OS and control samples. (B) RT-PCR was performed to assess the expression of XPR1 in OS and control samples. (TIF 81 kb)
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Mao, X., Guo, S., Gao, L. et al. Circ-XPR1 promotes osteosarcoma proliferation through regulating the miR-214-5p/DDX5 axis. Human Cell 34, 122–131 (2021). https://doi.org/10.1007/s13577-020-00412-z
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DOI: https://doi.org/10.1007/s13577-020-00412-z