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Gelatin–rosin gum complex nanoparticles: preparation, characterization and colon targeted delivery of 5-fluorouracil

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Abstract

Gelatin-rosin gum complex nanoparticles (GGRNPs) have been synthesized by complex coacervation method. The particles were extensively characterized using Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), and particle size analysis based on dynamic light scattering (DLS). The GGRNP3 were stable and their average particle size was  ~ 153 nm. GGRNPs were evaluated as the carrier matrix for 5-fluoro uracil (5-FU), and the release behavior of 5-FU was examined by swelling and in vitro dissolution tests in simulated gastrointestinal fluid (SGF) for first 2 h followed by 14 h in simulated intestinal fluid (SIF). About 71% drug release was witnessed (SGF = 21%; SIF = 50%) over a time span of 16 h, in contrast to native gelatin or rosin gum where under identical conditions the release exhausted within 7 h and 10 h, respectively. The release profile followed the first-order kinetics and the diffusion exponent (n) values obtained from the Korsemeyer−Peppas model ranged between 0.5 < n < 0.8 (both in SGF and SIF), which indicated non-Fickian diffusion mechanism. A549 cell line was used to carry out MTT assay test for investigating the cell toxicity of the drug-loaded GGRNP3 (D GGRNP3) where DGGRNP3 exhibited greater toxicity as compared to GGRNP3.

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Acknowledgements

The first author wishes to acknowledge University Grant Commission, New Delhi, India (Ref. No. 20/12/2015 (ii) EU-V; Sr. No. 2121510345, Dated: 17.05.2016), for providing financial assistance Under Junior Research Fellowship (NET-UGC-JRF) Scheme. Authors thank IIT, Kanpur, for FESEM and XRD instrumental facilities. The authors acknowledge MNIT, Jaipur, for FTIR and MNNIT, Allahabad, for particle size analysis facilities.

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Correspondence to Vandana Singh.

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Joshi, S., Singh, V. Gelatin–rosin gum complex nanoparticles: preparation, characterization and colon targeted delivery of 5-fluorouracil. Chem. Pap. 74, 4241–4252 (2020). https://doi.org/10.1007/s11696-020-01231-0

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