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Enzymatic Synthesis of Aliphatic Primary ω-Amino Alcohols from ω-Amino Fatty Acids by Carboxylic Acid Reductase

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Abstract

Medium to long chain aliphatic amino alcohols have numerous applications in polymer-based industries. We herein report a biocatalytic reduction of amino fatty acids into corresponding amino alcohols using carboxylic acid reductase and E coli endogenous aldehyde reductases. Interestingly, employing this reaction in tandem with minimizing the hurdle of substrate insolubility, the biocatalysts successfully produced 5.76 mM 12-amino-1-dodecanol from 10 mM of corresponding 12-amino-dodecanoic acid. Finally, up to 9.17 mM of 8-amino-1-octanol was obtained in 24 h on a preparative scale reaction of 50 mM substrate.

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Acknowledgements

This work was supported by the Ministry of Trade, Industry and Energy of South Korea (MOTIE, South Korea) under the industrial Technology Innovation Program, No. 10062550 and 10076343.The authors also acknowledge the funding from ‘Konkuk University Researcher Fund’ in 2019.

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Authors and Affiliations

  1. Department of Systems Biotechnology, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul, 050-29, South Korea

    Sharad Sarak, Hyunwoo Jeon, Mahesh D. Patil, Taresh P. Khobragade, Amol D. Pagar, Sihyong Sung, Sung Ho Yoon & Hyungdon Yun

  2. Bioengineering Institute, Seoul National University, Seoul, 08826, South Korea

    Hee-Wang Yoo & Byung-Gee Kim

  3. School of Chemical and Biological Engineering, Seoul National University, Seoul, 08826, South Korea

    Byung-Gee Kim

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  1. Sharad Sarak
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  2. Hyunwoo Jeon
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Correspondence to Hyungdon Yun.

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Sarak, S., Jeon, H., Patil, M.D. et al. Enzymatic Synthesis of Aliphatic Primary ω-Amino Alcohols from ω-Amino Fatty Acids by Carboxylic Acid Reductase. Catal Lett 150, 3079–3085 (2020). https://doi.org/10.1007/s10562-020-03233-9

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