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Reconstruction of the Fluorescence Spectra of Bisretinoids and the Products of Their Photooxidation and Photodegradation from the Retinal Pigment Epithelium of the Human Eye

  • CHEMICAL PHYSICS OF BIOLOGICAL PROCESSES
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Abstract

A global analysis of the fluorescence decay kinetics of a chloroform extract of lipofuscin granules from the retinal pigment epithelium cells of human cadaver eyes is performed. The analysis makes it possible to reconstruct the individual fluorescence properties of four groups of fluorophores belonging to bisretinoids and the products of their photooxidation and photodegradation. The characteristic fluorescence lifetimes are correlated with the spectral characteristics of the indicated fluorophore groups using the averaged spectral and kinetic characteristics of the chloroform extract.

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Funding

This study was supported by the no. 42 “Fundamental research for biomedical technologies” of the Presidium of the Russian Academy of Sciences.

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Authors and Affiliations

  1. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, 119991, Moscow, Russia

    M. A. Yakovleva, A. Sh. Radchenko, A. A. Kostyukov, V. A. Kuzmin, T. B. Feldman & M. A. Ostrovsky

  2. Fedorov Intersectoral Research and Technology Complex “Eye Microsurgery,” Ministry of Health of the Russian Federation, 127486, Moscow, Russia

    P. M. Arbukhanova & S. A. Borzenok

  3. Moscow State University, 119991, Moscow, Russia

    T. B. Feldman & M. A. Ostrovsky

Authors
  1. M. A. Yakovleva
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  2. A. Sh. Radchenko
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  3. A. A. Kostyukov
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  4. P. M. Arbukhanova
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  5. S. A. Borzenok
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  6. V. A. Kuzmin
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  7. T. B. Feldman
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  8. M. A. Ostrovsky
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Corresponding author

Correspondence to M. A. Yakovleva.

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Translated by N. Smolina

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Yakovleva, M.A., Radchenko, A.S., Kostyukov, A.A. et al. Reconstruction of the Fluorescence Spectra of Bisretinoids and the Products of Their Photooxidation and Photodegradation from the Retinal Pigment Epithelium of the Human Eye. Russ. J. Phys. Chem. B 14, 462–467 (2020). https://doi.org/10.1134/S199079312003029X

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  • DOI: https://doi.org/10.1134/S199079312003029X

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