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Combined chitosan and Cympobogon citratus (D.C. ex Nees) Stapf. essential oil to inhibit the fungal phytopathogen Paramyrothecium roridum and control crater rot in melon (Cucumis melo L.)

  • Food Microbiology - Research Paper
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Abstract

This study evaluated the efficacy of combined chitosan (Chi) and Cymbopogon citratus (DC) Stapf. essential oil (CCEO) to inhibit the fungal phytopathogen Paramyrothecium roridum L. Lombard & Crous and control crater rot in melon (Cucumis melo L.). Effects of several Chi and CCEO concentrations to inhibit the growth of four P. roridum isolates in vitro, as well as the type of interaction of some combined concentrations of Chi and CCEO was evaluated. Effects of coatings with combined concentrations of Chi and CCEO on development of crater rot lesions in melon artificially inoculated with P. roridum during storage (15 days, 25 °C) were measured. Chi (2.5, 3.75, 5, and 6.75 mg/mL) and CCEO (0.3 and 0.6 μL/mL) led to growth inhibition of the four examined P. roridum isolates. Combinations of Chi (5 mg/mL) and CCEO (0.15 and 0.3 μL/mL) had additive interaction to inhibit P. roridum. Coatings with additive combined concentrations of Chi and CCEO decreased the development and severity of carter rot lesions in melon during room storage regardless of the inoculated P. roridum isolate. Therefore, application of coatings formulated with combined concentrations of Chi and CCEO could be alternative strategies to control crater rot caused by P. roridum in melon and decrease synthetic fungicide use in this fruit.

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Funding

The authors received partial funding of this research (Finance code 001) from the CAPES (Brazil), as well as for the PhD scholarships awarded to authors S.A.M. and G.A.L.

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Authors and Affiliations

  1. Laboratório de Micologia, Departamento de Agronomia, Universidade Federal Rural de Pernambuco, Recife, Brazil

    Samara Alves Macedo & Marcos Paz Saraiva Câmara

  2. Laboratório de Microbiologia de Alimentos, Departamento de Nutrição, Centro de Ciências da Saúde, Universidade Federal da Paraíba, Campus I, 58051-900, Cidade Universitária, Joao Pessoa, Paraíba, Brazil

    Giovanna Alencar Lundgren, Selma dos Passos Braga & Evandro Leite de Souza

Authors
  1. Samara Alves Macedo
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  2. Giovanna Alencar Lundgren
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  3. Selma dos Passos Braga
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  4. Evandro Leite de Souza
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  5. Marcos Paz Saraiva Câmara
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Contributions

Conceptualization: S.A. Macêdo, E.L. de Souza, M.P.S. Câmara; Data curation; Formal analysis; Funding acquisition: E.L. de Souza, M.P.S. Câmara; Investigation: S.A. Macêdo, G.A. Lundgren, S.P. Braga; Methodology; S.A. Macêdo, G.A. Lundgren, S.P. Braga, E.L. de Souza, M.P.S. Câmara; Project administration: E.L. de Souza, M.P.S. Câmara; Resources: S.A. Macêdo, E.L. de Souza, M.P.S. Câmara; Supervision: E.L. de Souza, M.P.S. Câmara; Validation: S.A. Macêdo, G.A. Lundgren, S.P. Braga; Visualization: S.A. Macêdo, G.A. Lundgren, S.P. Braga, E.L. de Souza, M.P.S. Câmara; Writing - original draft: S.A. Macêdo, E.L. de Souza, M.P.S. Câmara; Writing—review and editing: E.L. de Souza, M.P.S. Câmara.

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Correspondence to Evandro Leite de Souza.

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Macedo, S.A., Lundgren, G.A., dos Passos Braga, S. et al. Combined chitosan and Cympobogon citratus (D.C. ex Nees) Stapf. essential oil to inhibit the fungal phytopathogen Paramyrothecium roridum and control crater rot in melon (Cucumis melo L.). Braz J Microbiol 51, 2057–2065 (2020). https://doi.org/10.1007/s42770-020-00378-y

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