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Recent Advances in Synthetic Biology for the Engineering of Lactic Acid Bacteria

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Abstract

Lactic acid bacteria (LAB) have a long historical record of usage in many types of food-related bioprocesses, and as LAB have been found to have intrinsic beneficial effects on human health, interest in using LAB as a medical treatment has increased. Moreover, recent advances in synthetic biology have enabled the engineering of so-called “smart” bacteria with the native characteristics of LAB. With various synthetic parts for gene expression, genome editing, and genetic circuits, gene expression can be precisely regulated in LAB. Moreover, LAB have been successfully developed as potential hosts for therapeutics to detect and control specific diseases or to deliver drug molecules. In this review, we outline the recent development of synthetic parts and approaches for the engineering of LAB. We also discuss the potential application of synthetic biology for the utilization of LAB as living therapeutics.

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Abbreviations

S.:

cerevisiae: Saccharomyces cerevisiae

E.:

coli: Escherichia coli

L.:

lactis: Lactococcus lactis

Lb.:

casei: Lactobacillus casei

Lb.:

plantarum: Lactobacillus plantarum

Lb:

acidophilus: Lactobacillus acidophilus

Lb.:

brevis: Lactobacillus brevis

Lb.:

delbrueckii: Lactobacillus delbrueckii

Lb.:

fermentum: Lactobacillus fermentum

Lb.:

helveticus: Lactobacillus helveticus

Lb.:

pentosus: Lactobacillus pentosus

Lb.:

reuteri: Lactobacillus reuteri

Lb.:

sakei: Lactobacillus sakei

Ln.:

mesenteroides: Leuconostoc mesenteroides

Ln.:

cremoris: Leuconostoc cremoris

Ln.:

citreum: Leuconostoc citreum

V.:

cholerae: Vibrio cholerae

E.:

faecalis: Enterococcus faecalis

S.:

aureus: Staphylococcus aureus

AmpR :

ampicillin resistance

CmR :

chloramphenicol resistance

EmR :

erythromycin resistance

KmR :

kanamycin resistance

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Acknowledgements

This work was supported by the Intelligent Synthetic Biology Center of the Global Frontier Project (Grant no. NRF-2014M3A6A8066443) funded by the Ministry of Science and ICT (MSIT)

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  1. Department of Chemical and Biomolecular Engineering, BK21 Plus program, KAIST, Daejeon, 34141, Korea

    Jaewoo Son & Ki Jun Jeong

  2. Institute for The BioCentury, KAIST, Daejeon, 34141, Korea

    Ki Jun Jeong

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  1. Jaewoo Son
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  2. Ki Jun Jeong
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Correspondence to Ki Jun Jeong.

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Son, J., Jeong, K.J. Recent Advances in Synthetic Biology for the Engineering of Lactic Acid Bacteria. Biotechnol Bioproc E 25, 962–973 (2020). https://doi.org/10.1007/s12257-020-0033-6

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