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Sequence analyses of RT-PCR products obtained from seven infected leaf samples revealed existence of three potyvirus species in Indian narcissus (Narcissus tazetta L.)

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Abstract

Potyvirus species associated with yellow leaf stripe disease of Indian narcissus (Narcissus tazetta L.) var. Paperwhite has been studied by sequence analyses of ~ 1.5 kb genomic fragments obtained from seven RT-PCR amplifications of infected samples. Sequence analysis revealed the occurrence of three potyvirus species: cyrtanthus elatus virus-A (CEVA: KF430815, KF430816, KM066973, KM066974); narcissus yellow stripe virus (NYSV: KM066972, JQ686724) and narcissus degeneration virus (NDV: MK572806). The existence of three potyvirus species: CEVA, NYSV and NDV are being reported in Indian narcissus.

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Acknowledgements

Thanks are due to the Director, CSIR-NBRI, Lucknow, India for necessary laboratory facilities. R. Raj is thankful to the University Grant Commission for Rajiv Gandhi National Fellowships and AcSIR for Ph.D. Thanks are also due to Dr. R. K. Roy, the Head, Floriculture Department, CSIR-NBRI, Lucknow for allowing narcissus leaf sample collection. This study is funded by the In-house project OLP105.

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Authors and Affiliations

  1. Plant Virus Laboratory, CSIR-National Botanical Research Institute, Lucknow, 226001, India

    Rashmi Raj, Charanjeet Kaur, Ashish Srivastava, Susheel Kumar & S. K. Raj

  2. Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, UP, 201002, India

    Rashmi Raj

  3. Amity Institute of Virology and Immunology, Amity University Uttar Pradesh, Noida, UP, 201313, India

    Ashish Srivastava

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  1. Rashmi Raj
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  2. Charanjeet Kaur
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Correspondence to S. K. Raj.

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Raj, R., Kaur, C., Srivastava, A. et al. Sequence analyses of RT-PCR products obtained from seven infected leaf samples revealed existence of three potyvirus species in Indian narcissus (Narcissus tazetta L.). 3 Biotech 10, 428 (2020). https://doi.org/10.1007/s13205-020-02418-8

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