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Fluid flow in an enclosed cavity of rotational bioreactor for bone tissue engineering

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Thermophysics and Aeromechanics Aims and scope

Abstract

The developed mathematical model was applied for study of fluid dynamics in a rotational bioreactor for bone tissue engineering by in vitro technology. The research goal is finding an optimal mode for rotation ensuring proper cyclic loading from fluid upon the cell-seeded biomaterial. The basis for developing a mathematical model of a bioreactor was a design of rotational type biological reactor used in medical research; the liquid flow is generated through viscosity mechanism due to surface rotation. Mathematical description of flow in a reactor cavity was performed with Navier—Stokes equations. It was assumed that flow regime in the boundary layer is laminar. Numerical algorithm was accomplished using a fluid flow solver “Fluent” in the code package ANSYS-12. Four variants of generating the rotational motion in the reactor cavity were considered. A series of parametric computations was performed for the rotation frequency f in the range 0.05 ≤ f ≤ 0.25 Hz. The paper offers visualization of velocity fields in the vertical plane. The distributions for shear stress and pressure in the working zone of reactor were calculated and analyzed. Simulations demonstrated that a method of fluid rotation by driving the outer cylinder with an offset axis is the best for arranging a cyclic pressure and cyclic shear stress on the biological material.

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References

  1. W. Yu, H. Qu, G. Hu, Q. Zhang, K. Song, H. Guan, T. Liu, and J. Qin A microfluidic based multi-shear device for investigating the effects of low fluid-induced stresses on osteoblasts, PLoS ONE, 2014, Vol. 9, No. 2, P. e89966–1–e89966–7.

    Article  ADS  Google Scholar 

  2. P. Li, Y.-C. Ma, X.-Y. Sheng, H.-T. Dong, H. Han, J. Wang, and Y.-Y. Xia, Cyclic fluid shear stress promotes osteoblastic cells proliferation through ERK5 signaling pathway, Mol. Cell. Biochem., 2012, Vol. 364, Nos. 1–2, P. 321–327.

    Article  Google Scholar 

  3. Z. Bo, G. Bin, W. Jing, W. Cuifang, A. Liping, M. Jinglin, J. Jin, T. Xiaoyi, C. Cong, D. Ning, and X. Yayi, Fluid shear stress promotes osteoblast proliferation via the Gaq-ERK5 signaling pathway, Connect. Tissue Res., 2016, Vol. 57, No. 4, P. 299–306.

    Article  Google Scholar 

  4. G. Bin, W. Cuifang, Z. Bo, W. Jing, J. Jin, T. Xiaoyi, C. Cong, C. Yonggang, A. Liping, M. Jinglin, and X. Yayi, Fluid shear stress inhibits TNF-α-induced osteoblast apoptosis via ERK5 signaling pathway, Biochem. Biophys. Res. Commun., 2015, Vol. 466, No. 1, P. 117–123.

    Article  Google Scholar 

  5. L.D. Landau and E.M. Lifshits, Fluid Mechanics, Elsevier, 2013.

  6. H. Schlichting, Boundary Layer Theory, 6th Edition, McGraw Hill, New York, 1968.

    MATH  Google Scholar 

  7. V.L. Ganimedov, E.O. Papaeva, N.A. Maslov, and P.M. Larionov, Mathematical model of a rotational bioreactor for the dynamic cultivation of scaffold-adhered human mesenchymal stem cells for bone regeneration, AIP Conf. Proc. 2017, Vol. 1882, No. 1, P. 20020–1–20020–6.

    Article  Google Scholar 

  8. V.L. Ganimedov, E.O. Papaeva, N.A. Maslov, and P.M. Larionov, Numerical simulation of fluid flow model of a rotational bioreactor for the dynamics in a rotational bioreactor, AIP Conf. Proc. 2017. Vol. 1883, No. 1, P. 30006–1–30006–7.

    Article  Google Scholar 

  9. V.L. Ganimedov, E.O. Tsibulskaya, N.A. Maslov, and P.M. Larionov, Modeling of fluid flow in a biological reactor of rotational type, Thermophysics and Aeromechanics, 2018, Vol. 25, No. 2, P. 211–218.

    Article  ADS  Google Scholar 

  10. R.G. Bacabac, T.H. Smit, S.C. Cowin, J.J. Van Loon, F.T. Nieuwstadt, R. Heethaar, and J. Klein-Nulend, Dynamic shear stress in parallel-plate flow chambers, J. Biomech., 2005, Vol. 38, No. 1, P. 159–167.

    Article  Google Scholar 

  11. E. Fröhlich, G. Bonstingl, A. Höfler, C. Meindl, G. Leitinger, T.R. Piebera, and E. Roblegg, Comparison of two in vitro systems to assess cellular effects of nanoparticles-containing aerosols, Toxicol. In Vitro, 2013, Vol. 27, No. 1, P. 409–417.

    Article  Google Scholar 

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Authors and Affiliations

  1. Novosibirsk State University, Novosibirsk, Russia

    P. M. Larionov

  2. Khristianovich Institute of Theoretical and Applied Mechanics SB RAS, Novosibirsk, Russia

    V. L. Ganimedov, N. A. Maslov & E. O. Tsibulskaya

Authors
  1. P. M. Larionov
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  2. V. L. Ganimedov
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  3. N. A. Maslov
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  4. E. O. Tsibulskaya
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Corresponding author

Correspondence to V. L. Ganimedov.

Additional information

Research was performed in the framework of Program for fundamental scientific research within academies of science for 2013–2020 years (Project AAAA-A17-117030610126-4).

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Larionov, P.M., Ganimedov, V.L., Maslov, N.A. et al. Fluid flow in an enclosed cavity of rotational bioreactor for bone tissue engineering. Thermophys. Aeromech. 26, 901–909 (2019). https://doi.org/10.1134/S0869864319060118

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  • DOI: https://doi.org/10.1134/S0869864319060118

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